detecting heterogeneity of neuroblastomas with a whole genetic analysis using SNP array

• Project/Area Number: 20791301
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pediatric surgery
• Research Institution: Kyushu University
• Principal Investigator: RYOTA Souzaki Kyushu University, 大学病院, 医員 (10403990)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 神経芽腫 / SNPアレイ / 生物学的多様性 / マイクロダイゼクション / SNP array / heterogeniety

Research Abstract

We thought that a small change of dosage of the MYCN gene due to intratumoral heterogeniety and a chromosomal gain of MYCN gene affected the prognosis in neuroblastoma. Therefore, the microdissection and SNP array were performed to determine the dosage of the MYCN gene and we found tha cases with a small number of MYCN amplified cells and a large number of MYCN gain cells. It was thought that a judgment of more accurate MYCN amplification was necessary using LCM and SNP array analysis in future

Research Products

• [Journal Article] Risks and benefits of ending of mass screening for neuroblastoma at 6 months of age in Japan. (2009)
  • Author(s): Tajiri T, Souzaki R, Kinoshita Y, Tanaka S, Koga Y, Suminoe A, Matsuzaki A, Hara T, Taguchi T
  • Journal Title: J Pediatr Surg. 44(12) Pages: 2253-7
• [Journal Article] A preoperative evaluation for neo-infantile liver tumors using a three-dimensional reconstruction of multidetector row CT. (2009)
  • Author(s): Kinoshita Y, Souzaki R, Tajiri T, Ieiri S, Hashizume M, Taguchi T
  • Journal Title: Oncol Rep. 21(4) Pages: 881-6
• [Journal Article] 神経芽腫摘出術後に発症した術後腸重積症の1例 (2009)
  • Author(s): 宗崎良太, 他
  • Journal Title: 小児がん 46(3) Pages: 393-7
  • NAID: 110007359108
  • Peer Reviewed
• [Journal Article] A preoperative evaluation for neo-infantile liver tumors using a three-dimensional reconstruction of multidetector row CT. (2009)
  • Author(s): Kinoshita Y, et al
  • Journal Title: Oncol.Rep. 21(4) Pages: 881-6
  • Peer Reviewed
• [Journal Article] Risks and benefits of ending of mass screening for neuroblastoma at 6 months of age in Japan. (2009)
  • Author(s): Tajiri T, et al
  • Journal Title: J Pediatr Surg. 44(12) Pages: 2253-7
  • Peer Reviewed
• [Journal Article] A preoperative evaluation for neo-infantile liver tumors using a three-dimensional reconstruction of multidetector row CT (2009)
  • Author(s): Kinoshita Y, Souzaki R, et.al
  • Journal Title: Oncol Rep (In press)
  • Peer Reviewed
• [Journal Article] Clinical implications of a slight increase in the gene dosage of MYCN in neuroblastoma determined using quantitative PCR. (2008)
  • Author(s): Souzaki R, Tajiri T, Higashi M, Kinoshita Y, Tanaka S, Kohashi K, Tsuneyoshi M, Taguchi T
  • Journal Title: Pediatr Surg Int. 24(10) Pages: 1095-100
  • NAID: 10027863988
• [Journal Article] Diagnostic value of lectin reactive alpha-fetoprotein for neoinfantile hepatic tumors and malignant germ cell tumors: preliminary study. (2008)
  • Author(s): Kinoshita Y, Tajiri T, Souzaki R, Tatsuta K, Higashi M, Izaki T, Takahashi Y, Taguchi T
  • Journal Title: J Pediatr Hematol Oncol. 30(6) Pages: 447-50
• [Journal Article] Clinical implications of a slight increase in the gene dosage of MYCN in neuroblastoma determined using quantitative PCR (2008)
  • Author(s): Souzaki R, et.al.
  • Journal Title: Pediatr Surg Int 24(10) Pages: 1095-1100
  • NAID: 10027863988
  • Peer Reviewed
• [Journal Article] Diagnostic value of lectin reactive alpha-fetoprotein for neo-infantile hepatic tumors and malignant germ cell tumors-Preliminary Study- (2008)
  • Author(s): Kinoshita Y, Tajiri T, Souzaki R, et.al
  • Journal Title: J Pediatr Hematol/Oncol 30 Pages: 447-450
  • Peer Reviewed
• [Journal Article] 進行神経芽腫治療に対する臓器温存手術と術後体外照射の意義 (2008)
  • Author(s): 田尻達郎、宗崎良太、木下義晶、田中桜、田口智章
  • Journal Title: 小児外科 40 Pages: 624-628
  • Peer Reviewed
• [Journal Article] 生物学的予後因子解析からみたマススクリーニングの功罪 (2008)
  • Author(s): 田尻達郎、宗崎良太、木下義晶、田中桜、東真弓、田口智章
  • Journal Title: 小児外科 40 Pages: 1047-1053
  • Peer Reviewed
• [Presentation] マイクロダイゼクションとSNP arrayを用いた神経芽腫の悪性度の層別化 (2009)
  • Author(s): 宗崎良太, 他
  • Organizer: 小児がん学会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-29
• [Presentation] MYCN gainと2p gain (2009)
  • Author(s): 宗崎良太, 他
  • Organizer: 日本小児外科学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-05-31
• [Presentation] The correlation between MYCN gain detected by FISH and distal 2p gain detected by SNP array in neuroblastoma (2009)
  • Author(s): Souzaki R, et al
  • Organizer: Pasific Association of Pediatric surgerons
  • Place of Presentation: Hongkong
  • Year and Date: 2009-05-14
• [Presentation] FISH法におけるMYC NgainとSNP arrayにおける2p gainの意義 (2009)
  • Author(s): 宗崎良太、田尻達郎、田中桜、木下義晶、田口智章
  • Organizer: 神経芽腫研究会
  • Place of Presentation: 築地、がんセンター研究所
  • Year and Date: 2009-03-14
• [Presentation] MYCN gainと2p gain (2009)
  • Author(s): 宗崎良太, 他
  • Organizer: 第46回日本小児外科学会
  • Place of Presentation: 大阪
• [Presentation] The correlation between MYCN gain detected by FISH and distal 2p gain detected by SNP array in neuroblastoma. (2009)
  • Author(s): Souzaki R, et al.
  • Organizer: Pasific Association of Pediatric surgerons
  • Place of Presentation: Hongkong
• [Presentation] マイクロダイゼクションとSNP arrayを用いた神経芽腫の悪性度の層別化. (2009)
  • Author(s): 宗崎良太, 他
  • Organizer: 第25回小児がん学会
  • Place of Presentation: 千葉
• [Presentation] 神経芽腫におけるMYCN増幅、MYCN gain, 2p gainの検討 (2008)
  • Author(s): 宗崎良太、田尻達郎、田中桜、木下義晶、田口智章
  • Organizer: 第24回小児がん学会
  • Place of Presentation: 千葉県幕張
  • Year and Date: 2008-11-14
• [Presentation] 神経芽腫における定量的PCR法で判定されたMYCN遺伝子量微量増加の意義 (2008)
  • Author(s): 宗崎良太、田尻達郎、田中桜、木下義晶、田口智章
  • Organizer: 第45回日本小児外科学会
  • Place of Presentation: つくば
  • Year and Date: 2008-05-29
• [Presentation] Clinical implications of the slight increase for the gene dosage of MYCN determi ned using quantitative PCR in neuroblastoma (2008)
  • Author(s): Souzaki R, Tajiri T, Kinoshita Y, Tanaka S, Taguchi T
  • Organizer: 13th Advances in Neuroblastoma Research
  • Place of Presentation: 幕張
  • Year and Date: 2008-05-23
• [Presentation] Clinical implications of the slight increase for the gene dosage of MYCN determined using quantitative PCR in neuroblastoma. (2008)
  • Author(s): Souzaki R, et al.
  • Organizer: ANR 2008
  • Place of Presentation: Makuhari
• [Presentation] 神経芽腫における定量的PCR法で判定されたMYCN遺伝子量微量増加の意義 (2008)
  • Author(s): 宗崎良太, 他
  • Organizer: 第45回日本小児外科学会
  • Place of Presentation: 筑波
• [Presentation] 神経芽腫におけるMYCN増幅、MYCN gain, 2p gainの検討 (2008)
  • Author(s): 宗崎良太, 他
  • Organizer: 第24回小児がん学会
  • Place of Presentation: 幕張