| Project/Area Number |
20791318
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plastic surgery
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| Research Institution | Kyorin University |
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Principal Investigator |
KINOSHITA Mikio Kyorin University, 医学部, 助教 (50422308)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 脂肪幹細胞 / 脂肪幹細胞移植 / 血管新生 / 脂肪幹細胞培養 / regenerative flap / 骨髄幹細胞移植 / 遊離皮弁 |
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| Research Abstract |
We tried to transplant the cultured adiposal stem cells (ASCs) into the various types of ischemic models, and evaluated the optimum conditions of wound bed for transplantation. In 2008, we created a skin chamber system in the back of mouse as a model for ASC transplantation. In 2009, we standardized the technique for the culture of the ASCs which had harvested from the groin region of the Wistar rat. Next, the cultured ASCs were immunostained and were injected to the back of originated mouse. By using the fluorescent microscope, we could confirm that the ASCs were colonized at the injected region. As a ischemic model, we made a single pedicle flap by the area of all dorsal surface in the back of Wistar Rat. The flaps which had injected ASCs showed apparently more expanded survival area than non-injected control models. We confirmed that ASCs could improve the circulation of the ischemic tissues and expand the survival area of the ischemic flaps.
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