The global analysis of N-α-acetylome that is aiming development of antitumor therapy by the target factor in oral cancer.

• Project/Area Number: 20791511
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Tottori University
• Principal Investigator: TAKUBO Kazuko Tottori University, 医学部附属病院, 助教 (90379628)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: アセチローム / αアセチル化 / 細胞死 / 細胞周期 / 5-FU / NATH / プロテオーム解析

Research Abstract

N-acetyltransferase human (NATH) participates in a posttranslational modification of the proteins and has been reported to play a role in apoptosis. In this study, the involvement of NATH in the cytotoxic action of 5-fluorouracil (5-FU) in human squamous cell carcinoma HEp-2 cells was examined. We found that 5-FU decreased NATH expression in a dose-dependent and time-dependent manner. No change was observed after treatment with other chemotherapeutics. The results of this study suggest that NATH plays an important role in the cytotoxic activity of 5-FU.

Research Products

• [Journal Article] Involvement of N-acetyltransferase human in the cytotoxic activity of 5-fluorouracil (2009)
  • Author(s): Takubo K
  • Journal Title: Anticancer Drugs 20 Pages: 668-675
  • Peer Reviewed
• [Journal Article]
  • Author(s): Kazuko Takubo, Hiroyuki Tsuchiya, Akihiro Kurimasa, Thomas Arnesen, Kazuo Ryoke, Goshi Shiota
  • Journal Title: Anticancer drugs Vol.20
  • Peer Reviewed
• [Presentation] Involvement of N-α-acetyltransferase human in the cytotoxic activity of 5-fluorouracil (2009)
  • Author(s): 田窪千子
  • Organizer: 日本癌学会第68回総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-10-03
• [Presentation] Involvement of N-α-acetyltransferase human in the cytotoxic activity of 5-fluorouracil. (2009)
  • Author(s): 田窪千子
  • Organizer: 日本癌学会第68回総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-10-03
• [Presentation] N-a-アセチルトランスフェラーゼを介した5-フルオロウラシルによる細胞毒性活性 (2009)
  • Author(s): 田窪 千子
  • Organizer: 第8回中四国口腔癌研究会学術講演会
  • Place of Presentation: ラヴィール岡山
  • Year and Date: 2009-08-28
• [Presentation] N-α-アセチルトランスフェラーゼを介した5-フルオロウラシルによる細胞毒性活性 (2009)
  • Author(s): 田窪千子
  • Organizer: 第8回中四国口腔癌研究会学術講演会
  • Place of Presentation: ラヴィール岡山
  • Year and Date: 2009-08-28
• [Presentation] 5-フルオロウラシルの細胞毒性活性におけるN-a-アセチルトランスフェラーゼの関与 (2009)
  • Author(s): 田窪 千子
  • Organizer: 第19回日本サイトメトリー学会
  • Place of Presentation: 松江テルサ
  • Year and Date: 2009-06-22
• [Presentation] Involvement of N-α-acetyltransferase human in the antitumor effect of 5-fluorouracil (2008)
  • Author(s): 田窪千子
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2008-12-12
• [Presentation] Involvement of N-α-acetyltransferase human in the antitumor effect of 5-fluorouracil (2008)
  • Author(s): 田窪千子
  • Organizer: 日本癌学会第67回総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-29
• [Presentation] Involvement of N-α-acetyltransferase human in the antitumor effect of 5-fluorouracil (2008)
  • Author(s): 田窪千子
  • Organizer: 日本癌学会第67回総会
  • Place of Presentation: 名古崖国際会議場
  • Year and Date: 2008-10-29
• [Presentation] 5-フルオロウラシルの抗腫瘍作用におけるN-a-アセチルトランスフェラーゼの関与 (2008)
  • Author(s): 田窪千子
  • Organizer: 第53回(社)日本口腔外科学会総会
  • Place of Presentation: アスティー徳島
  • Year and Date: 2008-10-21