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Differentiation induction of bone marrow stem cells into tooth component cells

Research Project

Project/Area Number 20791515
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionOkayama University

Principal Investigator

TSUJIGIWA Hidetsugu  Okayama University, 大学院・医歯薬学総合研究科, 助教 (70335628)

Research Collaborator NAGATUSKA Hitoshi  岡山大学, 大学院・医歯薬学総合研究科, 教授 (70237535)
YAMADA Masao  岡山大学, 大学院・医歯薬学総合研究科, 教授 (40166731)
Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords再生医学 / 骨髄幹細胞 / 歯 / GFP / 分化 / 肝細胞 / 歯髄細胞 / 骨髄細胞 / 再生医療 / 細胞分化 / 幹細胞 / 歯髄 / 骨髄
Research Abstract

This research was performed as a fundamental study for the clinical application of tooth regenerative medicine. In this research, we explored the possibility of guided differentiation induction of tooth component cells from bone marrow stem cells. The results revealed that bone marrow stem cells might possess differentiation capacity into tooth component cells and periodontal ligament. Moreover, stem cells in the dental pulp might bear similar characteristics with bone marrow stem cells. All the results demonstrated that the possible utilization of bone marrow stem cells in the prospective tooth regeneration medicine.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (34 results)

All 2009 2008

All Journal Article (23 results) (of which Peer Reviewed: 23 results) Presentation (11 results)

  • [Journal Article] Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells2009

    • Author(s)
      Rodriguez AP, Tsujigiwa H, Gunduz M, Cengiz B, Nagai N, Tamamura R, Borkosky SS, Takagi T, Inoue M, Nagatsuka H
    • Journal Title

      Biocell 33(1)

      Pages: 39-47

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prolactin may stimulate proliferation in the olfactory epithelium of the female mouse2009

    • Author(s)
      Orita S, Yoshinobu J, Orita Y, Tsujigiwa H, Kakiuchi M, Nagatsuka H, Nomiya S, Nagai N, Nishizaki K
    • Journal Title

      Am J Rhinol Allergy 23(2)

      Pages: 135-8

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Secreted frizzled related protein (sFRP)-2 inhibits bone formation and promotes cell proliferation in ameloblastoma2009

    • Author(s)
      Sathi GA, Inoue M, Harada H, Rodriguez AP, Tamamura R, Tsujigiwa H, Borkosky SS, Gunduz M, Nagatsuka H
    • Journal Title

      Oral Oncol 45(10)

      Pages: 856-860

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recombinant human bone morphogenetic protein-2/atelocollagen composite as a new material for ossicular reconstruction2009

    • Author(s)
      Takeuchi A, Tsujigiwa H, Murakami J, Kawasaki A, Takeda Y, Fukushima K, Rodriguez AP, Nagatsuka H, Yamada M, Nishizaki K
    • Journal Title

      J Biomed Mater Res A 89(1)

      Pages: 36-45

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The engraftment of transplanted bone marrow-derived cells into the inner ear2009

    • Author(s)
      Orita Y, Tsujigiwa H, Nishizaki K, Teshima T, Yoshinobu J, Orita S, Takeuchi A, Takeda Y, Nagatsuka H, Nagai N
    • Journal Title

      Eur Arch Otorhinolaryngol 266(1)

      Pages: 59-63

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immobilized recombinant human bone morphogenetic protein-2 enhances the phosphorylation of receptor-activated Smads2009

    • Author(s)
      Yamachika E, Tsujigiwa H, Shirasu N, Ueno T, Sakata Y, Fukunaga J, Mizukawa N Yamada M, Sugahara T
    • Journal Title

      J Biomed Mater Res A 88(3)

      Pages: 599-607

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression and Characterization of PGD(2) Receptors in Chronic Rhinosinusitis: Modulation of DP and CRTH2 by PGD(2)2009

    • Author(s)
      Yamamoto M, Okano M, Fujiwara T, Kariya S, Higaki T, Nagatsuka H, Tsujigiwa H, Yamada M, Yoshino T, Urade Y, Nishizaki K
    • Journal Title

      Int Arch Allergy Immunol 148(2)

      Pages: 127-136

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Heparanase and its related molecules in odontogenic tumors2009

    • Author(s)
      Nagatsuka H, Katase N, Han PP, Tsujigiwa H, Siar CH, Nakajima M, Naomoto Y, Tamamura R, Kawakami T, Gunduz M
    • Journal Title

      Oral Med Pathol 13(3)

      Pages: 81-89

    • NAID

      130004850527

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells2009

    • Author(s)
      Rodriguez AP
    • Journal Title

      Biocell 33(1)

      Pages: 39-47

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recombinant human bone morphogenetic protein-2/atelocollagen co mposite as a new material for ossicular reconstruction2009

    • Author(s)
      Takeuchi A
    • Journal Title

      J Biomed Mater Res A 89(1)

      Pages: 36-45

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The engraftment of transplanted bone marrow-derived cells into the inner ear2009

    • Author(s)
      Orita Y
    • Journal Title

      Eur Arch Otorhinolaryngol 266(1)

      Pages: 59-63

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immobilized recombinant human bone morphogenetic protein-2 enhances the phosphorylation of receptor-activated Smads2009

    • Author(s)
      Yamachika E
    • Journal Title

      J Biomed Mater Res A 88(3)

      Pages: 599-607

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heparanase and its related molecules in odontogenic tumors2009

    • Author(s)
      Nagatsuka H
    • Journal Title

      Oral Med Pathol 13(3)

      Pages: 81-89

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical Characteristics of Odontogenic Neoplasms and Their Physiological Counterparts2009

    • Author(s)
      Nakano K
    • Journal Title

      J Hard Tissue Biol 17(3)

      Pages: 79-80

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sequential expressions of Notch1, Jagged2 and Math1 in molar tooth germ of mouse2008

    • Author(s)
      Borkosky SS, Nagatsuka H, Orita Y, Tsujigiwa H, Yoshinobu J, Gunduz M, Rodriguez AP, Missana LR, Nishizaki K, Nagai N
    • Journal Title

      Biocell 32(3)

      Pages: 251-258

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Notch signaling in benign and malignant ameloblastic neoplasms2008

    • Author(s)
      Nakano K, Siar CH, Tsujigiwa H, Nagatsuka H, Nagai N, Kawakami T
    • Journal Title

      Eur J Med Res 13(10)

      Pages: 476-480

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Heparanase and vascular endothelial growth factor expression in the progression of oral mucosal melanoma2008

    • Author(s)
      Rivera RS, Nagatsuka H, Siar CH, Gunduz M, Tsujigiwa H, Han PP, Katase N, Tamamura R, Ng KH, Naomoto Y, Nakajima M, Nagai N
    • Journal Title

      Oncol Rep 19(3)

      Pages: 657-661

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression of jagged2 in mandibular condylar cartilage development2008

    • Author(s)
      Shimizu T, Tsujigiwa H, Nagatsuka H, Nakano K, Okafuji N, Kurihara S, Nagai N, Kawakami T
    • Journal Title

      Eur J Med Res 13(1)

      Pages: 1-3

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] C-kit protein expression correlated with activating mutations in KIT gene in oral mucosal melanoma2008

    • Author(s)
      Rivera RS, Nagatsuka H, Gunduz M, Cengiz B, Gunduz E, Siar CH, Tsujigiwa H, Tamamura R, Han KN, Nagai N
    • Journal Title

      Virchows Arch 452(1)

      Pages: 27-32

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical Characteristics of Odontogenic Neoplasms and Their Physiological Counterparts2008

    • Author(s)
      Nakano K, Nagatsuka H, Tsujigiwa H, Gunduz M, Katase N, Siar CH, Kawakami T
    • Journal Title

      J. Hard Tissue Biol 17(3)

      Pages: 79-90

    • NAID

      110007081308

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Localization of Oxytalan fiber, Type III Collagen and BMP Family in Conventional and Desmoplastic Ameloblastoma2008

    • Author(s)
      Inoue M, Nagatsuka H, Tamamura R, Siar CH, Tsujigiwa H, Borkosky S, Fujii M, Nagai N, Setsu K
    • Journal Title

      J. Hard Tissue Biol 17(1)

      Pages: 23-30

    • NAID

      110006791912

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sequential expressions of Notch1, Jagged2 and Math 1 in molar toothgerm of mouse2008

    • Author(s)
      Borkosky SS
    • Journal Title

      Biocell 32(3)

      Pages: 251-258

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Notch signaling in benign and malignant ameloblastic neoplasms2008

    • Author(s)
      Nakano K
    • Journal Title

      Eur J Med Res 13(10)

      Pages: 476-480

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] in vitro条件下において細胞外基質を産生する象牙芽幹細胞2009

    • Author(s)
      辻極秀次、片瀬直樹、山近英樹、長塚仁アンドレアロドリゲス、原田英光、山田雅夫
    • Organizer
      第18回硬組織再生生物学会学術大会・総会
    • Place of Presentation
      札幌
    • Year and Date
      2009-09-05
    • Related Report
      2009 Final Research Report
  • [Presentation] in vitro条件下において細胞外基質を産生する象牙芽幹細胞2009

    • Author(s)
      辻極秀次
    • Organizer
      第18回硬組織再生生物学会学術大会・総会
    • Place of Presentation
      北海道
    • Year and Date
      2009-09-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] 骨髄由来間葉系幹細胞とヘルペスウイルス2009

    • Author(s)
      辻極秀次、吉田まり子、山田雅夫
    • Organizer
      第24回中国四国ウイルス研究科
    • Place of Presentation
      岡山
    • Related Report
      2009 Final Research Report
  • [Presentation] 骨髄由来間葉系幹細胞とヘル尺ヌライルス2009

    • Author(s)
      辻極秀次
    • Organizer
      第24回中国四国ウイルス研究科
    • Place of Presentation
      岡山
    • Related Report
      2009 Annual Research Report
  • [Presentation] 耳小骨再建材料としてのrhBMP-2/アテロコラーゲン複合体2008

    • Author(s)
      辻極秀次、武田靖志、長塚仁、アンドレアロドリゲス、片瀬直樹、西崎和則、山田雅夫
    • Organizer
      第17回硬組織再生生物学会学術大会・総会
    • Place of Presentation
      徳島
    • Year and Date
      2008-08-30
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] A peliminary comparative study of CaTiO3 prepared by different methods2008

    • Author(s)
      Rodriguez A P, Tamamura R, Borkosky S S, Inoue M, Tsujigiwa H, Nagatsuka H, Sathi Gul S A, Kidhimoto Etsuo, Akao M, Nagai N
    • Organizer
      第17回硬組織再生生物学会学術大会・総会
    • Place of Presentation
      徳島
    • Year and Date
      2008-08-30
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Microsatellite analysis of ING tumor suppressor genes in ameloblastoma2008

    • Author(s)
      Silvia Susana Borkosky, Hitoshi Nagatsuka, Mehmet Gunduz, Hidetsugu Tsujigiwa, Ryo Tamamura, Naoki Katase, Masae Fujii, Gul SanAra Sathi, Noriyuki Nagai
    • Organizer
      第17回硬組織再生生物学会学術大会・総会
    • Place of Presentation
      徳島
    • Year and Date
      2008-08-30
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Osteoblast-like and odontblast-like cell response in vitro and rat bone activity in vivo: Effect of F-substituted apatites2008

    • Author(s)
      Inoue M, LeGeros R Z, Nagatsuka H, Inoue M, Tsujigiwa H, Rodriguez A P, Nagai N
    • Organizer
      The 4th International Symposium on Apatites and Correlative Biomaterials
    • Place of Presentation
      Manila, Philippines
    • Related Report
      2009 Final Research Report
  • [Presentation] Characterization of CaTiO3-aC Prepared by Modified Thermal Decomposition Method2008

    • Author(s)
      Rodriguez A P, Inoue M, Tsujigiwa H, Hailong H, Kishimoto E, Gunduz M, Nagatsuka H, Akao M, Nagai N
    • Organizer
      The 4th International Symposium on Apatites and Correlative Biomaterials
    • Place of Presentation
      Manila, Philippines
    • Related Report
      2009 Final Research Report
  • [Presentation] Bone formation with immobilized rhBMP-22008

    • Author(s)
      Yamachika E, Tsujigiwa H, Matsubara M, Kaneda Y, Shirasu N, Ueno T, Mizukawa N
    • Organizer
      XIX Congress of the European Association for Cranio-Maxillo-Facial Surgery
    • Place of Presentation
      Bologna, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] 臍帯血幹細胞由来肥満細胞に対する単純ヘルペスウイルス1型(HSV-1)及びインフルエンザウイルスの感染2008

    • Author(s)
      吉田まり子
    • Organizer
      第23回ヘルペスウイルス研究会
    • Place of Presentation
      鳥取
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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