Differentiation induction of bone marrow stem cells into tooth component cells

• Project/Area Number: 20791515
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: TSUJIGIWA Hidetsugu Okayama University, 大学院・医歯薬学総合研究科, 助教 (70335628)
• Research Collaborator: NAGATUSKA Hitoshi 岡山大学, 大学院・医歯薬学総合研究科, 教授 (70237535) ; YAMADA Masao 岡山大学, 大学院・医歯薬学総合研究科, 教授 (40166731)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 再生医学 / 骨髄幹細胞 / 歯 / GFP / 分化 / 肝細胞 / 歯髄細胞 / 骨髄細胞 / 再生医療 / 細胞分化 / 幹細胞 / 歯髄 / 骨髄

Research Abstract

This research was performed as a fundamental study for the clinical application of tooth regenerative medicine. In this research, we explored the possibility of guided differentiation induction of tooth component cells from bone marrow stem cells. The results revealed that bone marrow stem cells might possess differentiation capacity into tooth component cells and periodontal ligament. Moreover, stem cells in the dental pulp might bear similar characteristics with bone marrow stem cells. All the results demonstrated that the possible utilization of bone marrow stem cells in the prospective tooth regeneration medicine.

Research Products

• [Journal Article] Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells (2009)
  • Author(s): Rodriguez AP, Tsujigiwa H, Gunduz M, Cengiz B, Nagai N, Tamamura R, Borkosky SS, Takagi T, Inoue M, Nagatsuka H
  • Journal Title: Biocell 33(1) Pages: 39-47
  • Peer Reviewed
• [Journal Article] Prolactin may stimulate proliferation in the olfactory epithelium of the female mouse (2009)
  • Author(s): Orita S, Yoshinobu J, Orita Y, Tsujigiwa H, Kakiuchi M, Nagatsuka H, Nomiya S, Nagai N, Nishizaki K
  • Journal Title: Am J Rhinol Allergy 23(2) Pages: 135-8
  • Peer Reviewed
• [Journal Article] Secreted frizzled related protein (sFRP)-2 inhibits bone formation and promotes cell proliferation in ameloblastoma (2009)
  • Author(s): Sathi GA, Inoue M, Harada H, Rodriguez AP, Tamamura R, Tsujigiwa H, Borkosky SS, Gunduz M, Nagatsuka H
  • Journal Title: Oral Oncol 45(10) Pages: 856-860
  • Peer Reviewed
• [Journal Article] Recombinant human bone morphogenetic protein-2/atelocollagen composite as a new material for ossicular reconstruction (2009)
  • Author(s): Takeuchi A, Tsujigiwa H, Murakami J, Kawasaki A, Takeda Y, Fukushima K, Rodriguez AP, Nagatsuka H, Yamada M, Nishizaki K
  • Journal Title: J Biomed Mater Res A 89(1) Pages: 36-45
  • Peer Reviewed
• [Journal Article] The engraftment of transplanted bone marrow-derived cells into the inner ear (2009)
  • Author(s): Orita Y, Tsujigiwa H, Nishizaki K, Teshima T, Yoshinobu J, Orita S, Takeuchi A, Takeda Y, Nagatsuka H, Nagai N
  • Journal Title: Eur Arch Otorhinolaryngol 266(1) Pages: 59-63
  • Peer Reviewed
• [Journal Article] Immobilized recombinant human bone morphogenetic protein-2 enhances the phosphorylation of receptor-activated Smads (2009)
  • Author(s): Yamachika E, Tsujigiwa H, Shirasu N, Ueno T, Sakata Y, Fukunaga J, Mizukawa N Yamada M, Sugahara T
  • Journal Title: J Biomed Mater Res A 88(3) Pages: 599-607
  • Peer Reviewed
• [Journal Article] Expression and Characterization of PGD(2) Receptors in Chronic Rhinosinusitis: Modulation of DP and CRTH2 by PGD(2) (2009)
  • Author(s): Yamamoto M, Okano M, Fujiwara T, Kariya S, Higaki T, Nagatsuka H, Tsujigiwa H, Yamada M, Yoshino T, Urade Y, Nishizaki K
  • Journal Title: Int Arch Allergy Immunol 148(2) Pages: 127-136
  • Peer Reviewed
• [Journal Article] Heparanase and its related molecules in odontogenic tumors (2009)
  • Author(s): Nagatsuka H, Katase N, Han PP, Tsujigiwa H, Siar CH, Nakajima M, Naomoto Y, Tamamura R, Kawakami T, Gunduz M
  • Journal Title: Oral Med Pathol 13(3) Pages: 81-89
  • NAID: 130004850527
  • Peer Reviewed
• [Journal Article] Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells (2009)
  • Author(s): Rodriguez AP
  • Journal Title: Biocell 33(1) Pages: 39-47
  • Peer Reviewed
• [Journal Article] Recombinant human bone morphogenetic protein-2/atelocollagen co mposite as a new material for ossicular reconstruction (2009)
  • Author(s): Takeuchi A
  • Journal Title: J Biomed Mater Res A 89(1) Pages: 36-45
  • Peer Reviewed
• [Journal Article] The engraftment of transplanted bone marrow-derived cells into the inner ear (2009)
  • Author(s): Orita Y
  • Journal Title: Eur Arch Otorhinolaryngol 266(1) Pages: 59-63
  • Peer Reviewed
• [Journal Article] Immobilized recombinant human bone morphogenetic protein-2 enhances the phosphorylation of receptor-activated Smads (2009)
  • Author(s): Yamachika E
  • Journal Title: J Biomed Mater Res A 88(3) Pages: 599-607
  • Peer Reviewed
• [Journal Article] Heparanase and its related molecules in odontogenic tumors (2009)
  • Author(s): Nagatsuka H
  • Journal Title: Oral Med Pathol 13(3) Pages: 81-89
  • Peer Reviewed
• [Journal Article] Immunohistochemical Characteristics of Odontogenic Neoplasms and Their Physiological Counterparts (2009)
  • Author(s): Nakano K
  • Journal Title: J Hard Tissue Biol 17(3) Pages: 79-80
  • Peer Reviewed
• [Journal Article] Sequential expressions of Notch1, Jagged2 and Math1 in molar tooth germ of mouse (2008)
  • Author(s): Borkosky SS, Nagatsuka H, Orita Y, Tsujigiwa H, Yoshinobu J, Gunduz M, Rodriguez AP, Missana LR, Nishizaki K, Nagai N
  • Journal Title: Biocell 32(3) Pages: 251-258
  • Peer Reviewed
• [Journal Article] Notch signaling in benign and malignant ameloblastic neoplasms (2008)
  • Author(s): Nakano K, Siar CH, Tsujigiwa H, Nagatsuka H, Nagai N, Kawakami T
  • Journal Title: Eur J Med Res 13(10) Pages: 476-480
  • Peer Reviewed
• [Journal Article] Heparanase and vascular endothelial growth factor expression in the progression of oral mucosal melanoma (2008)
  • Author(s): Rivera RS, Nagatsuka H, Siar CH, Gunduz M, Tsujigiwa H, Han PP, Katase N, Tamamura R, Ng KH, Naomoto Y, Nakajima M, Nagai N
  • Journal Title: Oncol Rep 19(3) Pages: 657-661
  • Peer Reviewed
• [Journal Article] Gene expression of jagged2 in mandibular condylar cartilage development (2008)
  • Author(s): Shimizu T, Tsujigiwa H, Nagatsuka H, Nakano K, Okafuji N, Kurihara S, Nagai N, Kawakami T
  • Journal Title: Eur J Med Res 13(1) Pages: 1-3
  • Peer Reviewed
• [Journal Article] C-kit protein expression correlated with activating mutations in KIT gene in oral mucosal melanoma (2008)
  • Author(s): Rivera RS, Nagatsuka H, Gunduz M, Cengiz B, Gunduz E, Siar CH, Tsujigiwa H, Tamamura R, Han KN, Nagai N
  • Journal Title: Virchows Arch 452(1) Pages: 27-32
  • Peer Reviewed
• [Journal Article] Immunohistochemical Characteristics of Odontogenic Neoplasms and Their Physiological Counterparts (2008)
  • Author(s): Nakano K, Nagatsuka H, Tsujigiwa H, Gunduz M, Katase N, Siar CH, Kawakami T
  • Journal Title: J. Hard Tissue Biol 17(3) Pages: 79-90
  • NAID: 110007081308
  • Peer Reviewed
• [Journal Article] Localization of Oxytalan fiber, Type III Collagen and BMP Family in Conventional and Desmoplastic Ameloblastoma (2008)
  • Author(s): Inoue M, Nagatsuka H, Tamamura R, Siar CH, Tsujigiwa H, Borkosky S, Fujii M, Nagai N, Setsu K
  • Journal Title: J. Hard Tissue Biol 17(1) Pages: 23-30
  • NAID: 110006791912
  • Peer Reviewed
• [Journal Article] Sequential expressions of Notch1, Jagged2 and Math 1 in molar toothgerm of mouse (2008)
  • Author(s): Borkosky SS
  • Journal Title: Biocell 32(3) Pages: 251-258
  • Peer Reviewed
• [Journal Article] Notch signaling in benign and malignant ameloblastic neoplasms (2008)
  • Author(s): Nakano K
  • Journal Title: Eur J Med Res 13(10) Pages: 476-480
  • Peer Reviewed
• [Presentation] in vitro条件下において細胞外基質を産生する象牙芽幹細胞 (2009)
  • Author(s): 辻極秀次、片瀬直樹、山近英樹、長塚仁アンドレアロドリゲス、原田英光、山田雅夫
  • Organizer: 第18回硬組織再生生物学会学術大会・総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-09-05
• [Presentation] in vitro条件下において細胞外基質を産生する象牙芽幹細胞 (2009)
  • Author(s): 辻極秀次
  • Organizer: 第18回硬組織再生生物学会学術大会・総会
  • Place of Presentation: 北海道
  • Year and Date: 2009-09-05
• [Presentation] 骨髄由来間葉系幹細胞とヘルペスウイルス (2009)
  • Author(s): 辻極秀次、吉田まり子、山田雅夫
  • Organizer: 第24回中国四国ウイルス研究科
  • Place of Presentation: 岡山
• [Presentation] 骨髄由来間葉系幹細胞とヘル尺ヌライルス (2009)
  • Author(s): 辻極秀次
  • Organizer: 第24回中国四国ウイルス研究科
  • Place of Presentation: 岡山
• [Presentation] 耳小骨再建材料としてのrhBMP-2/アテロコラーゲン複合体 (2008)
  • Author(s): 辻極秀次、武田靖志、長塚仁、アンドレアロドリゲス、片瀬直樹、西崎和則、山田雅夫
  • Organizer: 第17回硬組織再生生物学会学術大会・総会
  • Place of Presentation: 徳島
  • Year and Date: 2008-08-30
• [Presentation] A peliminary comparative study of CaTiO3 prepared by different methods (2008)
  • Author(s): Rodriguez A P, Tamamura R, Borkosky S S, Inoue M, Tsujigiwa H, Nagatsuka H, Sathi Gul S A, Kidhimoto Etsuo, Akao M, Nagai N
  • Organizer: 第17回硬組織再生生物学会学術大会・総会
  • Place of Presentation: 徳島
  • Year and Date: 2008-08-30
• [Presentation] Microsatellite analysis of ING tumor suppressor genes in ameloblastoma (2008)
  • Author(s): Silvia Susana Borkosky, Hitoshi Nagatsuka, Mehmet Gunduz, Hidetsugu Tsujigiwa, Ryo Tamamura, Naoki Katase, Masae Fujii, Gul SanAra Sathi, Noriyuki Nagai
  • Organizer: 第17回硬組織再生生物学会学術大会・総会
  • Place of Presentation: 徳島
  • Year and Date: 2008-08-30
• [Presentation] Osteoblast-like and odontblast-like cell response in vitro and rat bone activity in vivo: Effect of F-substituted apatites (2008)
  • Author(s): Inoue M, LeGeros R Z, Nagatsuka H, Inoue M, Tsujigiwa H, Rodriguez A P, Nagai N
  • Organizer: The 4th International Symposium on Apatites and Correlative Biomaterials
  • Place of Presentation: Manila, Philippines
• [Presentation] Characterization of CaTiO3-aC Prepared by Modified Thermal Decomposition Method (2008)
  • Author(s): Rodriguez A P, Inoue M, Tsujigiwa H, Hailong H, Kishimoto E, Gunduz M, Nagatsuka H, Akao M, Nagai N
  • Organizer: The 4th International Symposium on Apatites and Correlative Biomaterials
  • Place of Presentation: Manila, Philippines
• [Presentation] Bone formation with immobilized rhBMP-2 (2008)
  • Author(s): Yamachika E, Tsujigiwa H, Matsubara M, Kaneda Y, Shirasu N, Ueno T, Mizukawa N
  • Organizer: XIX Congress of the European Association for Cranio-Maxillo-Facial Surgery
  • Place of Presentation: Bologna, USA
• [Presentation] 臍帯血幹細胞由来肥満細胞に対する単純ヘルペスウイルス1型(HSV-1)及びインフルエンザウイルスの感染 (2008)
  • Author(s): 吉田まり子
  • Organizer: 第23回ヘルペスウイルス研究会
  • Place of Presentation: 鳥取