Analysis of information processing in visual cortex of awake rats with in vivo functional calcium imaging

• Project/Area Number: 20800077
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: KIMURA Rie The Institute of Physical and Chemical Research, 認知判断モデル連携ユニット, 研究員 (60513455)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: 一次視覚野 / カルシウムイメージング / 二光子レーザー走査型顕微鏡 / 方位選択性 / 個体動物 / 無麻酔 / 脳の状態 / 出入力相関 / 個体動物in vivo / マルチユニット記録

Research Abstract

I established a new experimental system to apply in vivo two-photon functional calcium imaging to adult rats in the unanesthetized/anesthetized, head-restrained condition. Using this system, I recorded responses in the visual cortex by visual stimulation. I used pigmented VGAT-Venus transgenic rats, in which GABAergic neurons express a fluorescent protein, and distinguished between inhibitory and excitatory neurons. During the recording, ECoG, the pupil of the eye, and behavior were monitored. I examined orientation selectivity, direction selectivity, and maximum responses, and revealed different properties between an unanesthetized and anesthetized condition.

Research Products

• [Journal Article] 神経ネットワーク活動の可視化(特集「生命システムの階層間をまたぐイメージング技術」) (2009)
  • Author(s): 木村梨絵、池谷裕二
  • Journal Title: 蛋白質核酸酵素 54(15) Pages: 1952-1957
  • NAID: 40016894614
• [Journal Article] 神経ネットワーク活動の可視化 Visualization of neuronal network activity (2009)
  • Author(s): 木村梨絵、池谷裕二
  • Journal Title: 雑誌『蛋白質核酸酵素』Tanpakushitsu Kakusan Koso 54(15) Pages: 1952-1957
• [Journal Article] 多ニューロン活動の可視化(特集「学習と記憶-基礎と臨床」) (2008)
  • Author(s): 木村梨絵、池谷裕二
  • Journal Title: BRAIN and NERVE [医学書院] 60(7) Pages: 747-754
  • NAID: 40016144436
• [Journal Article] Protein Kinase CK2 Modulates Synaptic Plasticity by Modification of Synaptic NMDA Receptors in the Hippocampus. (2008)
  • Author(s): Kimura, R., Matsuki, N.
  • Journal Title: Journal of Physiology (London) 586(13) Pages: 3195-3206
  • Peer Reviewed
• [Journal Article] 多ニューロン活動の可視化 Visualization of Multineuronal Spike Activity (2008)
  • Author(s): 木村梨絵, 池谷裕二
  • Journal Title: BRAIN and NERVE 60(7) Pages: 747-754
• [Journal Article] Protein kinase CK2 modulates synaptic plasticity by modification of synaptic NMDA receptors in the hippocampus (2008)
  • Author(s): Rie Kimura, Norio Matsuki
  • Journal Title: Journal of Physiology 586(13) Pages: 3195-3206
  • Peer Reviewed
• [Presentation] 二光子カルシウムイメージングによって明らかになった、覚醒ラット視覚野の抑制性と興奮性神経細胞の光反応性 (2010)
  • Author(s): 木村梨絵、惣谷和広、蝦名鉄平、礒村宜和、柳川右千夫、加藤英之、津本忠治
  • Organizer: 第33回日本神経科学大会第53回日本神経化学会大会第20回日本神経回路学会大会合同大会
• [Presentation] 海馬多シナプス回路における情報演算の短期的および長期的可塑性 Short-term and long-term remodeling of information processing in hippocampal polysynaptic networks (2008)
  • Author(s): 木村梨絵, 松木則夫, 池谷裕二
  • Organizer: 第31回日本神経科学学会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] 海馬多シナプス回路における情報演算の短期的および長期的可塑性 (2008)
  • Author(s): 木村梨絵、松木則夫、池谷裕二
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Presentation] Visualization of hippocampal polysynaptic computation (2008)
  • Author(s): Kimura R, Matsuki N, Ikegaya Y
  • Organizer: NIPS-JST国際ワークショップP-5岡崎
  • Place of Presentation: 愛知県
• [Presentation] Visualization of hippocampal polysynaptic computation (2008)
  • Author(s): Rie Kimura, Norio Matsuki, Yuji Ikegaya
  • Organizer: NIPS-JST国際ワークショップ
  • Place of Presentation: 岡崎(愛知県)