Functional analyses of DNA polymerases in nucleotide excision repair

• Project/Area Number: 20810021
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Nagasaki University
• Principal Investigator: OGI Tomoo Nagasaki University, 大学院・医歯薬学総合研究科, 助教 (80508317)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: DNA修復 / ヌクレオチド除去修復

Research Abstract

Nucleotide excision repair (NER) removes the UV photolesions from DNA. Compromised NER activity causes xeroderma pigmentosum, a skin cancer predisposition genetic disorder. The early step of NER is well established, whereas the later step, which involves repair synthesis and nick ligasion, is not well understood. Here we demonstrate that three DNA polymerases coordinately role in the NER repair synthesis.

Research Products

• [Journal Article] Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells (2010)
  • Author(s): 荻朋男(責任著者), S. Limsirichaikul, R. Overmeer, M. Volker, K. Takenaka, R. Cloney, Y. Nakazawa, A. Niimi, Y. Miki, N. Jaspers, L. Mullenders, S. Yamashita, M. Fousteri, A. Lehmann
  • Journal Title: Molecular Cell 37 Pages: 714-27
  • NAID: 120006984627
  • Peer Reviewed
• [Journal Article] A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives (2010)
  • Author(s): Nakazawa, Y., S. Yamashita, A. Lehmann, 荻朋男(責任著者)
  • Journal Title: DNA Repair 9 Pages: 506-16
  • NAID: 120006984626
  • Peer Reviewed
• [Journal Article] Three DNA Polymerases, Recruited by Different Mechanisms, Carry Out NER Repair Synthesis in Human Cells (2010)
  • Author(s): Ogi, et al.
  • Journal Title: Molecular Cell 37 Pages: 714-727
  • NAID: 120006984627
  • Peer Reviewed
• [Journal Article] A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives (2010)
  • Author(s): Nakazawa, et al.
  • Journal Title: DNA Repair 9 Pages: 506-516
  • Peer Reviewed
• [Journal Article] A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethynyl deoxyuridine (EdU) (2009)
  • Author(s): Limsirichaikul, S., A. Niimi, H. Fawcett, A. Lehmann, S. Yamashita, 荻朋男(責任著者)
  • Journal Title: Nucleic Acids Research 37 Pages: 1-10
  • NAID: 120006982811
  • Peer Reviewed
• [Journal Article] A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethyny1 deoxyuridine (EdU) (2009)
  • Author(s): Limsirichaikul, et al.
  • Journal Title: Nucleic Acids Research 37
  • Peer Reviewed
• [Presentation] Three DNA Polymerases, Recruited by Different Mechanisms, Carry Out NER Repair Synthesis in Human Cells (2010)
  • Author(s): Tomoo Ogi
  • Organizer: International Conference on Radiation and Cancer Biology at Nagasaki 2010
  • Place of Presentation: 長崎
  • Year and Date: 2010-02-18
• [Presentation] ヌクレオチド除去修復過程での修復DNA合成分子メカニズムの解析 (2008)
  • Author(s): 荻 朋男
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
• [Patent(Industrial Property Rights)] 損傷DNA修復物質のスクリーニング方法 (2010)
  • Inventor(s): 荻朋男, 他2名
  • Industrial Property Rights Holder: 長崎大学
  • Filing Date: 2010-01-28
  • Overseas
• [Patent(Industrial Property Rights)] 損傷DNA修復物質のスクリーニング方法 (2009)
  • Inventor(s): 荻朋男, 他2名
  • Industrial Property Rights Holder: 長崎大学
  • Industrial Property Number: 2009-172521
  • Filing Date: 2009-07-23
• [Patent(Industrial Property Rights)] 日本国特許 (2009)
  • Inventor(s): 荻, ら
  • Industrial Property Rights Holder: 長崎大学
  • Industrial Property Number: 2009-172521
  • Filing Date: 2009-07-23