The role of kinetochore protein, Blinkin, for the localization and function of mitotic checkpoint proteins

• Project/Area Number: 20870023
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Kyoto University
• Principal Investigator: KIYOMITSU Tomomi Kyoto University, 生命科学研究科, 研究員 (10503443)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: 染色体分配 / 動原体 / M期チェックポイント / 分裂期チェックポイント / yeast 2 hybrid法 / ブリンキン / hMis12複合体 / hMis14 / Heterochromatin Protein-1(HP1) / Bubl

Research Abstract

Mitotic checkpoint ensures the equal segregation of chromosomes during mitosis. We identified minimal binding region of blinkin with mitotic checkpoint proteins, Bub1 and BubR1, and showed this domain is functionally important. Furthermore we identified blinkin binding kinetochore protein, hMis14, and showed hMis14-HP (heterochromatin protein)-1 interaction is mutually required for their interaction and functions. These results provide the new concept that HP1-dependent inner centromre assembly could affect the kinetochore assembly and mitotic checkpoint.

Research Products

• [Journal Article] Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes (2010)
  • Author(s): Tomomi Kiyomitsu, Osamu Iwasaki, Chikashi Obuse, Mitsuhiro Yanagida
  • Journal Title: The Journal of Cell Biology誌 vol.188 Pages: 791-807
  • NAID: 120002949726
  • Peer Reviewed
• [Journal Article] Inner centromere formation requires hMis14, a trident kinetochore prote in that specifically recruits HP1 to human chromosomes (2010)
  • Author(s): Tomomi Kiyomitsu, Osamu Iwasaki, Chikashi Obuse, Mitsuhiro Yanagida
  • Journal Title: The Journal of Cell Biology 188 Pages: 791-807
  • Peer Reviewed
• [Journal Article] ブリンキン-紡錘体形成チェックポイントと微小管結合に必須なキネトコア蛋白質- (2009)
  • Author(s): 清光智美、小布施力史、柳田充弘
  • Journal Title: 蛋白質核酸酵素 vol54-4 Pages: 421-426
• [Journal Article] The crystal structure of the N-terminal region of BUB1 provides insight into the mechanism of BUB1 recruitment to kinetochores (2009)
  • Author(s): Bolanos-Garcia, V.M., Kiyomitsu, T., D'Arcy, S., Chirgadze, D.Y., Grossmann, J.G., Matak-Vinkovic, D., Venkitaraman, A.R., Yanagida, M., Robinson, C.V., Blundell, T.L.
  • Journal Title: Structure誌 vol17 Pages: 105-116
  • Peer Reviewed
• [Journal Article] ブリンキン・紡錘体形成チェックポイントと微小管結合に必須なキネトコア蛋白質・ (2009)
  • Author(s): 清光智美, 小布施力史, 柳田充弘
  • Journal Title: 蛋白質 核酸 酵素 54-4 Pages: 421-426
  • Peer Reviewed
• [Journal Article] The crystal structure of the N-terminal region of BUB1 provides insight into the mechanism of BUB1 recruitment to kinetochores (2009)
  • Author(s): Bolanos-Garcia VM, Kiyomitsu T, D'Arcy S, Chirgadze DY, Grossmann JG, Matak-Vinkovic D, Venkitaraman AR, Yanagida M, Robinson CV, Blundell TL
  • Journal Title: Structure 17 Pages: 105-116
  • Peer Reviewed
• [Presentation] キネトコアとインナーセントロメア形成の相互依存性 (2009)
  • Author(s): 清光智美
  • Organizer: 先端生命科学シンポジウム染色体の分子構築
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-06-26
• [Presentation] キネトコアとインナーセントロメア形成の相互依存性 (2009)
  • Author(s): 清光智美
  • Organizer: 先端生命科学シンポジウム 染色体の分子構築
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-06-26
• [Presentation] Mutual dependency between kinetochore and inner centromere assemblies (2009)
  • Author(s): Tomomi Kiyomitsu
  • Organizer: The 24th Naito Conference on " Nuclear dynamics and RNA(II)"
  • Place of Presentation: シャトレーゼガトーキングダム札幌
  • Year and Date: 2009-06-25
• [Presentation] Mutual dependency between kinetochore and inner centromere assemblies (2009)
  • Author(s): 清光智美
  • Organizer: The 24^<th> Naito Conference on "Nuclear dynamics and RNA (II)"
  • Place of Presentation: シャトレーゼガトーキングダム札幌
  • Year and Date: 2009-06-25
• [Presentation] ヒトキネトコアタンパク質hMis14とHP1の直接結合がM期染色体のインナーセントロメア形成に必要である (2009)
  • Author(s): 清光智美
  • Organizer: 第8回核ダイナミクス研究会
  • Place of Presentation: ラフォーレ修善時静岡県伊豆市大平1529
  • Year and Date: 2009-06-20
• [Presentation] Inner centromere formation requires trident-binding kinetochore protein (2009)
  • Author(s): Kiyomitsu, T., Iwasaki, O., Obuse, C., Yanagida, M.
  • Organizer: hMis14, that specifically recruits HP1 in human chromosome
  • Place of Presentation: Spindle Assembly and Function, Lucca, Italy
• [Presentation] Human kinetochore protein, blinkin/AF15q14, is required for chromosome alignment and the mitotic checkpoint through direct interaction with Bub1 and BubR1 (2008)
  • Author(s): Tomomi Kiyomitsu, Chikashi Obuse, Mitsuhiro Yanagida
  • Organizer: CONFERENCES JACQUES-MONOD, The cell cycle and genomic stability
  • Place of Presentation: France, Roscoff
  • Year and Date: 2008-04-27