Cell-specific regulation of the JC virus late gene

• Project/Area Number: 20890005
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Hokkaido University
• Principal Investigator: ORBA Yasuko Hokkaido University, 人獣共通感染症リサーチセンター, 博士研究員 (60507169)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: JCウイルス / T抗原 / カフェイン / 細胞特異性 / Tag

Research Abstract

(1) Large T antigen of JC virus activates various promoters including JC virus late promoter. The enhancement of promoter activity by large T antigen is prominent in JC virus permissive cells. The cell-specific transcriptional regulation by T antigen is one of potential mechanisms of cell specificity for JC virus infection.
(2) We found that caffeine had a pronounced antiviral effect in cells infected with JC virus. Caffeine suppressed expression of viral early proteins and viral DNA replication.

Research Products

• [Journal Article] Large T antigen promotes JC virus replication in G2-arrested cells by inducing ATM-and ATR-mediated G2 checkpoint signaling (2010)
  • Author(s): Yasuko Orba, Tadaki Suzuki, Yoshinori Makino, Kanako Kubota, Shinya Tanaka, Takashi Kimura, Hirofumi Sawa
  • Journal Title: J. Biol. Chem. 285 Pages: 1544-1554
  • Peer Reviewed
• [Journal Article] Large T antigen promotes JC virus replication in G2-arrested cells by inducing ATM- and ATR-mediated G2 checkpoint signaling (2010)
  • Author(s): Orba Y, Suzuki T, Makino Y, Kubota K, Tanaka S, Kimura T, Sawa H.
  • Journal Title: The Journal of Biological Chemistry 285 Pages: 1544-1554
  • Peer Reviewed
• [Presentation] Large T antigen of JC virus promotes viral replication by inducing ATM- and ATR-mediated G2 checkpoint signaling. (2009)
  • Author(s): 大場靖子
  • Organizer: The 1st international young researcher seminar in zoonosis control
  • Place of Presentation: ニセコいこいの村
  • Year and Date: 2009-08-20
• [Presentation] Large T antigen of JC virus promotes viral replication by inducing ATM- and ATR-mediated G2 checkpoint signaling. (2009)
  • Author(s): 大場靖子
  • Organizer: The 1^<st> international young researcher seminar in zoonosis control
  • Place of Presentation: ニセコ町、いこいの村
  • Year and Date: 2009-08-20
• [Presentation] JCウイルスlarge T antigenによるG2/M checkpoint活性化機構の解析。 (2008)
  • Author(s): 大場靖子
  • Organizer: BMB2008
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] JCウイルスlarge T antigenによるG2/Mcheckpoint活性化機構の解析 (2008)
  • Author(s): 大場靖子
  • Organizer: BMB2008
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2008-12-11
• [Presentation] ヒトポリオーマウイルスJCVのLarge T Antigenによる細胞周期G2期停止機構。 (2008)
  • Author(s): 大場靖子
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2008-10-26
• [Presentation] ヒトポリオーマウイルスJCVのLarge T Antigenによる細胞周期G2期停止機構 (2008)
  • Author(s): 大場靖子
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山コンベンションセンター(岡山市)
  • Year and Date: 2008-10-26