Structural changes of junctions between lymphatic endothelial cells in lymphatic invasion

• Project/Area Number: 20890056
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: General surgery
• Research Institution: The University of Tokyo
• Principal Investigator: IWATA Caname The University of Tokyo, 大学院・医学系研究科, 助教 (50511391)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: 癌転移 / リンパ管 / 炎症 / 癌 / リンパ管新生 / がん転移 / 免疫

Research Abstract

We hypothesize that the junctions between lymphatic endothelial cells are dilated in tumor environment and that the dilation help the invasion of cancer cells. We used the murine model of peritoneal inflammation and observed the structural changes in lymphatic vessels on the diaphragms. Stomatas, which are the entrance of initial lymphatic vessels on diaphragm, are dilated after the i.p. injection of proinflammatory agent. And the dilation is completely inhibited by treatment of sorafenib, a multikinase inhibitor.

Research Products

• [Journal Article] Identification of targets of Prox1 during in vitro vascular diffe rentiation from embryonic stem cells : functional roles of HoxD8 in lymphan giogenesis (2010)
  • Author(s): Harada K, Yamazaki T, Iwata C, Yoshimatsu Y, Sase H, Mishima K, Morishita Y, Hirashima M, Oike Y, Suda T, Miura N, Watabe T, Miyazono K
  • Journal Title: Journal of Cell Science 112 Pages: 3923-3930
  • Peer Reviewed
• [Journal Article] inflammation induces lymphangiogenesis through up-regulation of VEGFR-3 mediated by NF-kappaB and Prox1 (2010)
  • Author(s): Flister MJ, Wilber A, Hall KL, Iwata C, Miyazono K, Nisato RE, Pepper MS, Zawieja DC, Ran S
  • Journal Title: Blood 115 Pages: 418-429
  • Peer Reviewed
• [Journal Article] Inflammation induces lymphangiogenesis through up-regulation of VEGFR-3 mediated by NF-kappaB and Prox1 (2009)
  • Author(s): Flister MJ, Wilber A, Hall KL, Iwata C, Miyazono K, Nisato RE, Pepper MS, Zawieja DC, Ran S.
  • Journal Title: Blood 115 Pages: 418-429
  • Peer Reviewed
• [Journal Article] Identification of targets of Prox1 during in vitro vascular differentiation from embryonic stem cells : functional roles of HoxD8 in lymphangiogenesis (2009)
  • Author(s): Harada K, Yamazaki T, Iwata C, Yoshimatsu Y, Sase H, Mishima K, Morishita Y, Hirashima M, Oike Y, Suda T, Miura N, Watabe T, Miyazono K
  • Journal Title: Journal of Cell Science 122 Pages: 3923-3930
  • Peer Reviewed
• [Journal Article] Inhibition of endogenous TGF-beta signaling enhances lymphangiogenesis (2008)
  • Author(s): Oka M, Iwata C, Suzuki HI, Kiyono K, Morishita Y, Watabe T, Komuro A, Kano MR, Miyazono K.
  • Journal Title: Blood 111 Pages: 4571-4579
  • Peer Reviewed
• [Journal Article] Inhibition of endogenous TGF-b signaling enhances lymphangiogenesis (2008)
  • Author(s): Oka, M., Iwata, C., Suzuki, H. I., Kiyono K., Moriskita, Y., Watabe, T., Komuro, A., Kano, M. R., and Miyazono, K.
  • Journal Title: Blood 111 Pages: 4571-4579
  • Peer Reviewed
• [Presentation] Microstructural change of lymphatic endothelial cells in metastatic lymph node (Poster) (2009)
  • Author(s): Iwata C, Kano MR, Yashiro M, Hirakawa K, Miyazono K.
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 癌リンパ管新生からみた、スキルス胃がんのリンパ行性転移および腹膜播種の病態についての基礎的研究 (2008)
  • Author(s): 岩田要, 八代正和, 平川弘聖, 宮園浩平, 上西紀夫
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎県長崎市長崎ブリックホール
  • Year and Date: 2008-05-17
• [Remarks]
  • URL: http://beta-lab.umin.ac.jp/
• [Remarks]
  • URL: http://beta-lab.umin.ac.jp/