Periodontitis vaccine effectiveness for elderly

• Project/Area Number: 20890123
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Social dentistry
• Research Institution: Osaka University
• Principal Investigator: SEKINE Shinichi Osaka University, 大学院・歯学研究科, 助教 (70506344)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,354,000 (Direct Cost: ¥2,580,000、Indirect Cost: ¥774,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,794,000 (Direct Cost: ¥1,380,000、Indirect Cost: ¥414,000)
• Keywords: 高齢者 / 歯周病 / ワクチン / 樹状細胞 / 粘膜ワクチン / Porphyromonas gingivalis

Research Abstract

The purpose of this investigation was to determine the efficacy of periodontitis vaccine for elderly. Our previous studies showed that nasal immunization induced mucosal immune responses in aging mice (one-year old) but not in aged (two-year old) mice when cholera toxin (CT) was employed as a nasal adjuvant. However, NALT DC-targeting mucosal adjuvants such as plasmid Flt3 ligand (pFL) and CpG ODN successfully induced antigen (Ag)-specific S-IgA Ab responses in aged mice. NALT DCs played an important role in this functional mechanism.

Research Products

• [Journal Article] Fusobacterium nucleatum envelope protein FomA is immunogenic and binds to the salivary statherin-derived peptide. (2010)
  • Author(s): Nakagaki, H. Sekine, S., Terao, Y., Toe, M., Tanaka, M., Ito, H.O., Kawabata, S., Shizukuishi, S., Fujihashi. K., Kataoka, K
  • Journal Title: Infection and Immunity 78 Pages: 1185-1192
  • Peer Reviewed
• [Journal Article] Association of ALDH2 genotypes with periodontitis progression. (2010)
  • Author(s): Nishida, N., Tanaka, M., Sekine, S., Takeshita, T., Nakayama, K., Morimoto, K., Shizukuishi, S
  • Journal Title: Journal of Dental Research 89 Pages: 138-142
  • Peer Reviewed
• [Journal Article] Fusobacterium nucleatum Envelope Protein FomA Is Immunogenic and Binds to the Salivary Statherin-Derived Peptide. (2010)
  • Author(s): Nakagaki, H.
  • Journal Title: Infection and Immunity 78 Pages: 1185-1192
  • Peer Reviewed
• [Journal Article] Association of ALDH2 Genotypes with Periodontitis Progression. (2010)
  • Author(s): Nishida, N.
  • Journal Title: Journal of Dental Research 89 Pages: 138-142
  • Peer Reviewed
• [Presentation] Antigen Presenting Cell Function of NALT DCs Is Maintained in Aged Mice. (2009)
  • Author(s): Sekine, S.
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2009-12-02
• [Presentation] CCR5- and CCR6-Positive Dendritic Cells Play A Central Role In Adenovirus Expressing Flt3 Ligand Induced Mucosal S-IgA Ab Responses. (2009)
  • Author(s): Fukuyama, Y.
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2009-12-02
• [Presentation] 粘膜アジュバントとして用いたFlt3 ligand cDNA及びCpG ODNの免疫誘導能解析 (2009)
  • Author(s): 関根伸一
  • Organizer: 第58回日本口腔衛生学会・総会
  • Place of Presentation: 岐阜市
  • Year and Date: 2009-10-11
• [Presentation] Oral Immunization with OVA Plus CT Induces Hyper IgA Ab Responses, Inflammatory Cytokines and Foxp3 Expressing CD4^+ T Cells in the Intestinal Lamina Propria of PIR-B Deficient Mice. (2009)
  • Author(s): Kobayashi, R.
  • Organizer: 14^<th> ICMI
  • Place of Presentation: Boston, USA
  • Year and Date: 2009-07-08
• [Presentation] NALT Derived-DCs Induce Innate B1-B Cells to Undergo IgA Class Switching Recombination. (2009)
  • Author(s): Kataoka, K.
  • Organizer: 14^<th> ICMI
  • Place of Presentation: Boston, USA
  • Year and Date: 2009-07-07
• [Presentation] Nasal Influenza Vaccine Combined with CpG-ODN and pFL as Mucosal Adjuvant Induces Two Subsets of Dendritic Cells for Th1-and Th2-type Cytokine Responses. (2009)
  • Author(s): Zamri, N.
  • Organizer: 14^<th> ICMI
  • Place of Presentation: Boston, USA
  • Year and Date: 2009-07-06
• [Presentation] High-cholesterol Diet Accelerates Inflammatory Cytokine Production in Apolipoprotein E-null Mice Infected with Porphyromonas Gingivalis. (2009)
  • Author(s): Sekine, S.
  • Organizer: 14^<th> ICMI
  • Place of Presentation: Boston, USA
  • Year and Date: 2009-07-06
• [Presentation] エリスリトールは歯周病原性細菌の揮発性硫化物産生を抑制する (2009)
  • Author(s): 橋野恵衣
  • Organizer: 第20回日本口腔衛生学会 近畿・中国・四国地方会総会
  • Place of Presentation: 広島市
  • Year and Date: 2009-06-21
• [Presentation] Adenovirus-FLT3 Induces DC-Mediated CD4+ T Cell-Activation for Salivary S-IgA Responses. (2009)
  • Author(s): Markham, J. Fukuyama, Y. Sekine, S. Gilbert, R.L., Fujihashi, K
  • Organizer: 87th IADR
  • Place of Presentation: Miami, USA
  • Year and Date: 2009-04-02
• [Presentation] Antigen Presenting Cell Function of NALT DCs Is Maintained in Aged Mice. (2009)
  • Author(s): Sekine, S. Sasaki, M. Fukuyama, Y. Kobayashi, R. Asanuma, H. Kataoka, K. Gilbert, R. Shizukuishi, S. Fujihashi, K
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: Osaka
• [Presentation] CCR5- and CCR6-Positive Dendritic Cells Play A Central Role In Adenovirus Expressing Flt3 Ligand Induced Mucosal S-IgA Ab Responses. (2009)
  • Author(s): Fukuyama, Y. Sekine, S. Kobayashi, R. Gilbert, R. Fujihashi, K
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: Osaka
• [Presentation] 雫石聰粘膜アジュバントとして用いたFlt3 ligand cDNA及びCpG ODNの免疫誘導能解析 (2009)
  • Author(s): 関根伸一, 佐々木美香, 片岡宏介, 岩崎未央, 橋野恵衣, 東江正裕
  • Organizer: 58th日本口腔衛生学会・総会
  • Place of Presentation: Gifu
• [Presentation] High-cholesterol diet accelerates inflammatory cytokine production in apolipoprotein E-null mice infected with Porphyromonas gingivalis. (2009)
  • Author(s): Sekine, S. Tanaka, M. Iwasaki, M. Toe, M. Hashino, E. Kataoka, K. Fujihashi K., Shizukuishi, S
  • Organizer: 14th ICMI
  • Place of Presentation: Boston, USA
• [Presentation] Nasal Influenza Vaccine Combined with CpG-ODN and pFL as Mucosal Adjuvant Induces Two Subsets of Dendritic Cells for Th1-and Th2-type Cytokine Responses. (2009)
  • Author(s): Zamri, N. Asanuma, H. Sekine, S. Kurata, T. Sata, T. Tamura, S., Fujihashi, K
  • Organizer: 14th ICMI
  • Place of Presentation: Boston, USA
• [Presentation] Mucosal DCs Activated by Nasal Cholera Toxin Induce Innate Bl-B Cellsto Undergo IgA CSR. (2008)
  • Author(s): Kataoka, K.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2008-12-03
• [Presentation] NALT CDllb^+ Dendritic Cell Migration and Balanced Thl- and Th2-Cytokine Responses Induce Antigen-Specific Immunity. (2008)
  • Author(s): Sekine, S.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2008-12-03
• [Presentation] 粘膜アジュバント・コレラ毒素の経鼻投与は唾液腺B1-B細胞のIgAクラススイッチングを誘導する, 粘膜アジュバント・コレラ毒素の経鼻投与は唾液腺B1-B細胞のIgAクラススイッチングを誘導する (2008)
  • Author(s): 片岡宏介, 関根伸一, 福井誠, 金川裕子, Tselmeg Baartarjav, 伊藤寛夫
  • Organizer: 第57回日本口腔衛生学会・総会
  • Place of Presentation: Saitama
  • Year and Date: 2008-10-03
• [Presentation] 粘膜アジュバント・コレラ毒素の経鼻投与は唾液腺B1-B細胞のIgAクラススイッチングを誘導する (2008)
  • Author(s): 片岡宏介
  • Organizer: 第57回日本口腔衛生学会・総会
  • Place of Presentation: さいたま市
  • Year and Date: 2008-10-03
• [Presentation] 金属特異的結合唾液タンパク質の同定 (2008)
  • Author(s): 片岡宏介
  • Organizer: 第19回日本口腔衛生学会近畿・中国・四国地方会総会
  • Place of Presentation: 徳島市
  • Year and Date: 2008-06-22
• [Presentation] 金属特異的結合唾液タンパク質の同定 (2008)
  • Author(s): 片岡宏介, 関根伸一, 東江正裕, 田中宗雄, 福井誠, 伊藤寛夫, 雫石聰
  • Organizer: 第19回日本口腔衛生学会近畿・中国・四国地方会総会
  • Place of Presentation: Tokushima
  • Year and Date: 2008-01-22
• [Presentation] NALT CD11b+ dendritic cell migration and balanced Th1- and Th2- cytokine responses induce antigen-specific immunity. (2008)
  • Author(s): Sekine, S. Fukuyama, Y. Kataoka, K. Fujihashi, K. Kobayashi, R. Davydova, J. Yamamoto, M. McGhee, J.R. Shizukuishi, S., Fujihashi, K
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: Kyoto
• [Presentation] K Mucosal DCs Activated by Nasal Cholera Toxin Induce Innate B1-B Cells to Undergo IgA CSR. (2008)
  • Author(s): Kataoka, K. Fujihashi, K. Sekine, S. Fukui, M. Kawabata S. McGhee, J.R. Ito H., Fujihashi
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: Kyoto