| Project/Area Number |
20F20084
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 45040:Ecology and environment-related
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| Research Institution | Kyoto University |
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Principal Investigator |
東樹 宏和 京都大学, 生態学研究センター, 准教授 (60585024)
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| Co-Investigator(Kenkyū-buntansha) |
CANARINI ALBERTO 京都大学, 生態学研究センター, 外国人特別研究員
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| Project Period (FY) |
2020-09-25 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2022: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2021: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2020: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | symbiosis / plants / mycorrhizal fungi / 共生 / 土壌 / 植物 / microbiomes / chemical ecology / stable isotopes / nutrient defficiency |
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| Outline of Research at the Start |
鉄欠乏土壌を対象とした現地調査とサンプリングを行い、本研究室で最適化したDNA抽出プロトコルを適用する。抽出DNAに対し、細菌16S rRNA領域、および真菌ITS領域のDNAメタバーコーディングを行い、日本の鉄欠乏土壌における微生物叢を解明する。そのうえで、群集生態学的な分析を実施し、鉄欠乏土壌以外の土地との間での比較分析を行う。植物根内に共生する微生物群集についても、随時分析を行う。さらに、土壌化学分析を並行して実施し、土壌生態系における物理化学性と生物性の間にかる関係性を探る。
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| Outline of Annual Research Achievements |
To finalize the research project, we have done the following experiments and analyses: 1) Experimental inoculation of endophytic fungi on model plant species: 2) Finalization of the tests for calibrating the necessary condition to successfully achieve stable isotope tracing techniques: these tests allowed us to characterize the necessary conditions to achieve measurable amounts of stable isotope tracer incorporated in different biomarkers for fungi and in plant biomass: 3) Carrying an in vitro experiment to test competition dynamics between different root endophytic fungi and arbuscular mycorrhizal fungi during nitrogen and carbon exchange with a model plant: this experiment allowed us to understand how endophytic fungi that carried similar function to AMF could modify the trading feature of carbon and nitrogen exchange in the AMF-plant symbiosis: 4) Carrying a pot experiment to validate the in vitro results: this experiment allowed us to generalize the findings obtained in in vitro conditions to soil-grown plants: 5) Transcriptomic analyses (RNA sequencing) of the model plant specimens used for the inoculation experiments of endophytic fungi. 6) In-silico analyses of the RNA-sequencing datasets mentioned above.
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| Research Progress Status |
令和4年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和4年度が最終年度であるため、記入しない。
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