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Transmission of antibiotics resistance genes in biological activated carbon advanced water treatment and its control

Research Project

Project/Area Number 20H00261
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 22:Civil engineering and related fields
Research InstitutionGifu University

Principal Investigator

LI FUSHENG  岐阜大学, 流域圏科学研究センター, 教授 (10332686)

Co-Investigator(Kenkyū-buntansha) 魏 永芬  岐阜大学, 流域圏科学研究センター, 准教授 (00467218)
山田 俊郎  北海学園大学, 工学部, 教授 (30335103)
石黒 泰  岐阜大学, 流域圏科学研究センター, 助教 (10743891)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥44,980,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥10,380,000)
Fiscal Year 2023: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2022: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2021: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2020: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Keywords水道水安全 / 高度浄水処理 / 薬剤耐性遺伝子 / 活性炭処理 / 金属担持活性炭 / 薬剤耐性菌伝播抑制 / 活性炭 / 水道水質安全 / 耐性遺伝子 / 生物膜 / 水質安全 / 活性炭吸着 / 生物活性炭 / 塩素処理 / オゾン酸化 / 浄水処理 / 細菌 / 細菌群集構造
Outline of Research at the Start

薬剤耐性菌は世界中で大きな課題となっている。安全・安心な水道水の供給のために、高度な浄水処理の実現の核心となる生物活性炭処理における薬剤耐性遺伝子の伝播の機構を解明し、薬剤耐性菌の出現と拡散の抑制に有効な手法を提案することが極めて重要で有意義である。本研究は、薬剤耐性遺伝子をコードしたプラスミドを吸着した活性炭と感受性細菌の共存培養を組み合せた室内実験、活性炭処理施設を導入している浄水処理システムに対する現場調査ならびに高度なデータ解析を通じて、活性炭処理施設内における耐性遺伝子の伝播の機構を解明するとともに、施設の操作運転条件、塩素処理やオゾン処理などの前処理の影響を明らかにする。

Outline of Final Research Achievements

For safe drinking water supply through lowering the risk from antibiotic resistance genes, research was conducted through laboratory experiment and field study to clarify the mechanisms related to the transfer of genes in biological activated carbon treatment process, to evaluate the impacts of coagulation, sedimentation, sand filtration, chlorination and ozone-oxidation and to propose metal-modified activated carbon to inhibit the transfer of genes; and some findings of reference value were obtained.

Academic Significance and Societal Importance of the Research Achievements

研究を通して、浄水処理工程における薬剤耐性菌の消長、それに関わる影響因子、影響機構が明らかになり、薬剤耐性遺伝子の抑制を含めた視点から原水の水質特徴に応じた浄水処理プロセスの操作運転条件の最適化手法と高度浄水処理システム全般の運転管理のあり方が示された。水源水質が複雑になりつつある状況下において、安全安心な水道水をどのように確保できるかが大きな課題である。本研究の成果はこの課題の解決に寄与するものである。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Comments on the Screening Results   Annual Research Report
  • Research Products

    (34 results)

All 2024 2023 2022 2021 2020

All Journal Article (18 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 15 results,  Open Access: 1 results) Presentation (15 results) (of which Int'l Joint Research: 3 results) Book (1 results)

  • [Journal Article] Fate and Behavior of Tetracycline Resistance Genes in Activated Carbon Adsorption2024

    • Author(s)
      Anggreini Sri、Aurellya Alma Rizky、Li Wenqing、Li Fusheng
    • Journal Title

      Journal of Water Resource and Protection

      Volume: 16 Issue: 01 Pages: 1-16

    • DOI

      10.4236/jwarp.2024.161001

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transfer, elimination and accumulation of antibiotic resistance genes in decentralized household wastewater treatment facility treating total wastewater from residential complex2024

    • Author(s)
      Su Haoning、Li Wenjiao、Okumura Shinya、Wei Yongfen、Deng Zhiyi、Li Fusheng
    • Journal Title

      Science of The Total Environment

      Volume: 912 Pages: 169144-169144

    • DOI

      10.1016/j.scitotenv.2023.169144

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Fluorescence-based indicators predict the performance of conventional drinking water treatment processes: Evaluation based on the changes in the compositions of dissolved organic matter2023

    • Author(s)
      Yusup Rosadi Maulana、Maysaroh Sutra、Diva Sagita Nadya、Anggreini Sri、Desmiarti Reni、Deng Zhiyi、Li Fusheng
    • Journal Title

      Chemosphere

      Volume: 337 Pages: 139410-139410

    • DOI

      10.1016/j.chemosphere.2023.139410

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Characteristics of organic matter released from drinking water treatment sludge under different storage conditions: Evaluation based on activated carbon adsorbability2023

    • Author(s)
      Anggreini Sri、Rosadi Maulana Yusup、Yamada Toshiro、Hudori Hudori、Deng Zhiyi、Li Fusheng
    • Journal Title

      Chemosphere

      Volume: 339 Pages: 139679-139679

    • DOI

      10.1016/j.chemosphere.2023.139679

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Spatiotemporal Variation of Fishy Smell-Causing Algae (Uroglena americana) and Its Correlation with Water Quality Factors in the Source Water of Drinking Water Treatment Plant2023

    • Author(s)
      Sarkar Kanika、Yandi Rama、Sagita Nadya Diva、Li Fusheng
    • Journal Title

      Water, Air, & Soil Pollution

      Volume: 234 Issue: 8 Pages: 507-507

    • DOI

      10.1007/s11270-023-06490-7

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relation analysis of bacterial community in soils of coal mines with potential ecological risk from heavy metals2023

    • Author(s)
      Wang Yajie、Li Jiefeng、Li Wenjiao、Wei Yongfen、Deng Zhiyi、Li Fusheng
    • Journal Title

      Environmental Technology & Innovation

      Volume: 30 Pages: 103125-103125

    • DOI

      10.1016/j.eti.2023.103125

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Characterization of DOM released from bacteria in response to chlorine in water based on indicator bacteria2022

    • Author(s)
      Sarkar Kanika、Wei Gengrui、Rosadi Maulana Yusup、Murata Naoki、Li Fusheng
    • Journal Title

      Environmental Technology

      Volume: 44 Issue: 2 Pages: 1-15

    • DOI

      10.1080/09593330.2022.2102939

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Accumulation capability for cesium differs among bacterial species: A comprehensive study using bacteria isolated from freshwater and coastal sediment2022

    • Author(s)
      Li Jiefeng、Wang Yajie、Li Wenjiao、Bhat Sartaj Ahmad、Wei Yongfen、Deng Zhiyi、Hao Xiaodi、Li Fusheng
    • Journal Title

      Environmental Pollution

      Volume: 292 Pages: 118431-118431

    • DOI

      10.1016/j.envpol.2021.118431

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Temperature impacts fate of antibiotic resistance genes during vermicomposting of domestic excess activated sludge2022

    • Author(s)
      Cui Guangyu、Fu Xiaoyong、Bhat Sartaj Ahmad、Tian Weiping、Lei Xuyang、Wei Yongfen、Li Fusheng
    • Journal Title

      Environmental Research

      Volume: 207 Pages: 112654-112654

    • DOI

      10.1016/j.envres.2021.112654

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Distribution of extracellular and intracellular antibiotic resistance genes in sludge fractionated in terms of settleability2021

    • Author(s)
      Li Wenjiao、Su Haoning、Li Jiefeng、Bhat Sartaj Ahmad、Cui Guangyu、Han Zaw Min、Nadya Diva Sagita、Wei Yongfen、Li Fusheng
    • Journal Title

      Science of The Total Environment

      Volume: 760 Pages: 143317-143317

    • DOI

      10.1016/j.scitotenv.2020.143317

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Elimination of antibiotic resistance genes from excess activated sludge added for effective treatment of fruit and vegetable waste in a novel vermireactor2021

    • Author(s)
      Li Wenjiao、Li Jiefeng、Ahmad Bhat Sartaj、Wei Yongfen、Deng Zhiyi、Li Fusheng
    • Journal Title

      Bioresource Technology

      Volume: 325 Pages: 124695-124695

    • DOI

      10.1016/j.biortech.2021.124695

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The storage temperature and oxygen concentration greatly affect organic matter released from drinking water treatment sludge: Evaluation based on the consumption rate for chlorine2021

    • Author(s)
      Rosadi Maulana Yusup、Maysaroh Sutra、Hudori Hudori、Sarkar Kanika、Yamada Toshiro、Li Fusheng
    • Journal Title

      Journal of Water Process Engineering

      Volume: 43 Pages: 102312-102312

    • DOI

      10.1016/j.jwpe.2021.102312

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of the Recycling Process on Drinking Water Treatment: Evaluation Based on Fluorescence EEM Analysis Using the Peak-Picking Technique and Self-Organizing Map2021

    • Author(s)
      Hudori Hudori、Rosadi Maulana Yusup、Yamada Toshiro、Bhat Sartaj Ahmad、Li Fusheng
    • Journal Title

      Water

      Volume: 13 Issue: 23 Pages: 3456-3456

    • DOI

      10.3390/w13233456

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of dissolved organic matter extracted from water treatment sludge2020

    • Author(s)
      Rosadi Maulana Yusup、Yamada Toshiro、Hudori Hudori、Tamaoki Hiroto、Li Fusheng
    • Journal Title

      Water Supply

      Volume: - Issue: 6 Pages: 2194-2205

    • DOI

      10.2166/ws.2020.120

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of dissolved organic matter at a water treatment plant with closed systems in different seasons2020

    • Author(s)
      Hudori Hudori、Yamada Toshiro、Suzuki Yukitaka、Rosadi Maulana Yusup、Tamaoki Hiroto、Li Fusheng
    • Journal Title

      Water Supply

      Volume: - Issue: 5 Pages: 2013-2020

    • DOI

      10.2166/ws.2020.117

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of excess activated sludge on vermicomposting of fruit and vegetable waste by using novel vermireactor2020

    • Author(s)
      Li Wenjiao、Bhat Sartaj Ahmad、Li Jiefeng、Cui Guangyu、Wei Yongfen、Yamada Toshiro、Li Fusheng
    • Journal Title

      Bioresource Technology

      Volume: 302 Pages: 122816-122816

    • DOI

      10.1016/j.biortech.2020.122816

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Elimination of antibiotic resistance genes and human pathogenic bacteria by earthworms during vermicomposting of dewatered sludge by metagenomic analysis2020

    • Author(s)
      Huang Kui、Xia Hui、Zhang Yingying、Li Jianhui、Cui Guangyu、Li Fusheng、Bai Wei、Jiang Yufeng、Wu Nan
    • Journal Title

      Bioresource Technology

      Volume: 297 Pages: 122451-122451

    • DOI

      10.1016/j.biortech.2019.122451

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Effect of heavy metals on the performance and bacterial profiles of activated sludge in a semi-continuous reactor2020

    • Author(s)
      Bhat Sartaj Ahmad、Cui Guangyu、Li Wenjiao、Wei Yongfen、Li Fusheng
    • Journal Title

      Chemosphere

      Volume: 241 Pages: 125035-125035

    • DOI

      10.1016/j.chemosphere.2019.125035

    • Related Report
      2020 Annual Research Report
  • [Presentation] Reduction of Antibiotic Resistance Genes in Large-Scale Johkasou Treating Residential Area Wastewater2023

    • Author(s)
      Su Haoning, Okumura Shinya and Li Fusheng
    • Organizer
      Proceedings of UGSAS-GU & BWEL Joint Poster Presentation on Agricultural and Basin Water Environmental Sciences 2023
    • Related Report
      2023 Annual Research Report
  • [Presentation] Fate and Behavior of Antibiotic Resistance Genes in Activated Carbon Adsorption2023

    • Author(s)
      Sri Anggreini, Alma Rizky Aurellya and Fusheng Li
    • Organizer
      Proceedings of UGSAS-GU & BWEL Joint Poster Presentation on Agricultural and Basin Water Environmental Sciences 2023
    • Related Report
      2023 Annual Research Report
  • [Presentation] Fate and behavior of antibiotic resistance genes when contact with powdered activated carbon2023

    • Author(s)
      Sri Anggreini, Fusheng Li
    • Organizer
      第23回環境技術学会年次大会研究発表会予稿集
    • Related Report
      2023 Annual Research Report
  • [Presentation] Changes of 16S rDNA and antibiotic resistance genes in conventional water treatment process2023

    • Author(s)
      Nadya Diva Sagita, Fusheng Li
    • Organizer
      第23回環境技術学会年次大会研究発表会予稿集
    • Related Report
      2023 Annual Research Report
  • [Presentation] Quantity and existing state changes of antibiotic resistance genes in large-scale Johkasou treating residential area wastewater2023

    • Author(s)
      Su Haoning, Fusheng Li
    • Organizer
      第23回環境技術学会年次大会研究発表会予稿集
    • Related Report
      2023 Annual Research Report
  • [Presentation] Potential impact of chlorination on the fate of antibiotic resistance genes in drinking water treatment2021

    • Author(s)
      Nadya Diva Sagita, Sutra Maysaroh, Maulana Yusup Rosadi, Fusheng Li
    • Organizer
      令和3年度(2021)日本水環境学会中部支部研究発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Changes of dissolved organic matter from source to treated water in drinking water treatment plant: Evaluation based on fluorescence excitation-emission matrix analysis2021

    • Author(s)
      Maulana Yusup Rosadi, Nadya Diva Sagita, Sutra Maysaroh, Li Fusheng
    • Organizer
      令和3年度(2021)日本水環境学会中部支部研究発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Seasonal changes of algal species in Nagara River2021

    • Author(s)
      Wenqing Li, Haoning Su, Showa Wakamiya, Fusheng Li
    • Organizer
      令和3年度(2021)日本水環境学会中部支部研究発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Coexisting and competing algal species with moldy odor-causing blue algal in Nagara River2021

    • Author(s)
      Wenqing Li, Haoning Su, Shoma Wakamiya and Fusheng Li
    • Organizer
      Proceedings of UGSAS-GU & BWEL Joint Poster Session on Agricultural and Basin Water Environmental Sciences
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Changes of 16S rDNA and antibiotic resistance genes in drinking water treatment process2021

    • Author(s)
      Nadya Diva Sagita, Sutra Maysaroh, Maulana Yusup Rosadi and Fusheng Li
    • Organizer
      Proceedings of UGSAS-GU & BWEL Joint Poster Session on Agricultural and Basin Water Environmental Sciences
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Changes of dissolved organic matter from source to treated water in drinking water treatment plant: Evaluation based on fluorescence excitation-emission matrix analysis2021

    • Author(s)
      Maulana Yusup Rosadi, Nadya Diva Sagita, Sutra Maysaroh and Li Fusheng
    • Organizer
      Proceedings of UGSAS-GU & BWEL Joint Poster Session on Agricultural and Basin Water Environmental Sciences
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Distribution of extracellular and intracellular antibiotic resistance genes in sludge fractionated in terms of settleability2020

    • Author(s)
      Wenjiao Li, Fusheng Li,
    • Organizer
      The 4th Symposium of River Basin Studies - Towards the Interdisciplinary Study of the Sustainable Utilization and Management of River Basin Systems -
    • Related Report
      2020 Annual Research Report
  • [Presentation] Advanced drinking water treatment by activated carbon: Evaluation based on organic matter removal and bacterial community in biofilm2020

    • Author(s)
      Hiroki Maruyama, Fusheng Li
    • Organizer
      The 4th Symposium of River Basin Studies - Towards the Interdisciplinary Study of the Sustainable Utilization and Management of River Basin Systems -
    • Related Report
      2020 Annual Research Report
  • [Presentation] Elimination of antibiotic resistance genes introduced from excess activated sludge in a novel vermireactor for treatment of fruit and vegetable waste2020

    • Author(s)
      Wenjiao Li, Fusheng Li
    • Organizer
      Proceedings of UGSAS-GU & BWEL Joint Poster Session on Agricultural and Basin Water Environmental Sciences
    • Related Report
      2020 Annual Research Report
  • [Presentation] Occurrence and behavior of antibiotic resistance genes during drinking water treatment process2020

    • Author(s)
      Nadya Diva Sagita, Wenjiao Li, Fusheng Li
    • Organizer
      Joint International Seminar of Xiangtan University and Gifu University on Environmental Science and Engineering
    • Related Report
      2020 Annual Research Report
  • [Book] River Basin Environment: Evaluation, Management and Conservation2022

    • Author(s)
      Li, F., Awaya, Y., Kageyama, K., Wei, Y. (eds)
    • Total Pages
      356
    • Publisher
      Springer
    • Related Report
      2022 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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