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Mechanical processing platform for single cells and single molecules using optically-driven nanotools

Research Project

Project/Area Number 20H02118
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 20020:Robotics and intelligent system-related
Research InstitutionKagawa University

Principal Investigator

TERAO KYOHEI  香川大学, 創造工学部, 准教授 (80467448)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2022: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2021: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2020: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywordsナノ加工 / 1細胞解析 / 1分子計測 / 生体分子加工 / マイクロ流体デバイス / マイクロ・ナノデバイス / バイオナノテクノロジー / 微細加工 / 1細胞解析 / 1分子解析
Outline of Research at the Start

切断や組立といった機械加工に相似した操作を、細胞1個と分子1個に対して実現することが本研究の狙いである。それにより細胞・分子を分解して解析し生命現象の理解に繋げること、細胞を改変・組み立てることで医薬品開発や再生医療において有用なバイオサンプルを創出する。そのために、酵素・抗体反応をピンポイントに生じさせる光駆動ナノ工具を開発し、加工対象の細胞・分子を配置固定可能なマイクロ流体ワークテーブル上で1個の細胞・1個の分子に対して機械加工を達成する。

Outline of Final Research Achievements

The objective of this study is to realize mechanical processes, such as cutting and assembling, on individual cells and molecules. This will enable us to disassemble and analyze cells and molecules, leading to a better understanding of biological phenomena. In addition, we can reassemble cells to develop biosamples that are beneficial for drug development and regenerative medicine. To achieve this, we have developed an optically-driven nano-tool that allows for precise induction of enzyme and antibody reactions. Additionally, we have developed technologies for working on a single cell or molecule using a microfluidic worktable. This allows us to position and secure the cell or molecule being processed.

Academic Significance and Societal Importance of the Research Achievements

本研究成果として開発した要素技術を統合することで、将来「1個の細胞・1個の生体分子を望み通りに捕捉し、解析する」「1個の細胞に物質を導入し性質を改変する」、「細胞を1個1個望み通りに組み立て、臓器を作製する」「1個の生体分子を望み通りに切断する」、といった機械加工に相似した操作を細胞と分子に対して1個の解像度で実現することに近づくことが期待される。それにより、1細胞・1分子解析への応用や、有用な細胞サンプルの調製に繋がる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (32 results)

All 2023 2022 2021 2020

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 3 results) Presentation (24 results) (of which Int'l Joint Research: 8 results)

  • [Journal Article] Microfluidic device for differentiation induction of iPS cells derived embryoid bodies with local chemical stimulation2023

    • Author(s)
      Kusunoki Naoto、Konagaya Shuhei、Nishida Mitsunori、Sato Shigehiro、Takao Hidekuni、Shimokawa Fusao、Terao Kyohei
    • Journal Title

      Electronics and Communications in Japan

      Volume: 106 Issue: 1

    • DOI

      10.1002/ecj.12393

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optically driven microtools with an antibody‐immobilised surface for on‐site cell assembly2023

    • Author(s)
      Mori Shuntaro、Ito Takumi、Takao Hidekuni、Shimokawa Fusao、Terao Kyohei
    • Journal Title

      IET Nanobiotechnology

      Volume: Online Version Issue: 3 Pages: 1-1

    • DOI

      10.1049/nbt2.12114

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microfluidic Device for Differentiation Induction of iPS Cells-derived Embryoid Bodies with Local Chemical Stimulation2022

    • Author(s)
      Kusunoki Naoto、Konagaya Shuhei、Nishida Mitsunori、Sato Shigehiro、Takao Hidekuni、Shimokawa Fusao、Terao Kyohei
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 142 Issue: 12 Pages: 310-315

    • DOI

      10.1541/ieejsmas.142.310

    • ISSN
      1341-8939, 1347-5525
    • Year and Date
      2022-12-01
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of optical‐driven nanoneedles using SU‐8 nanofabrication2022

    • Author(s)
      Harada Masahiko、Takao Hidekuni、Simokawa Fusao、Terao Kyohei
    • Journal Title

      Electronics and Communications in Japan

      Volume: 105 Issue: 1

    • DOI

      10.1002/ecj.12327

    • NAID

      210000188884

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] AC-Electroosmosis-Assisted Surface Plasmon Resonance Sensing for Enhancing Protein Signals with a Simple Kretschmann Configuration2022

    • Author(s)
      Terao Kyohei、Kondo Shohei
    • Journal Title

      Sensors

      Volume: 22 Issue: 3 Pages: 854-854

    • DOI

      10.3390/s22030854

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of Optical-Driven Nanoneedles Using SU-8 Nanofabrication2021

    • Author(s)
      Harada Masahiko、Takao Hidekuni、Simokawa Fusao、Terao Kyohei
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 141 Issue: 6 Pages: 173-178

    • DOI

      10.1541/ieejsmas.141.173

    • NAID

      130008046595

    • ISSN
      1341-8939, 1347-5525
    • Year and Date
      2021-06-01
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] On-site processing of single chromosomal DNA molecules using optically driven microtools on a microfluidic workbench2021

    • Author(s)
      Masuda Akihito、Takao Hidekuni、Shimokawa Fusao、Terao Kyohei
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 7961-7961

    • DOI

      10.1038/s41598-021-87238-3

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Capture and elongation of single chromosomal DNA molecules using optically driven microchopsticks2020

    • Author(s)
      Inukai Ryo、Takao Hidekuni、Shimokawa Fusao、Terao Kyohei
    • Journal Title

      Biomicrofluidics

      Volume: 14 Issue: 4 Pages: 044114-044114

    • DOI

      10.1063/5.0017727

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] Spatial Dissection of Fixed Tissue Slices Using Si Blade Array Device2022

    • Author(s)
      K. Ueta, H. Takao, F. Shimokawa, F. Miura, K. Terao
    • Organizer
      35th International Microprocesses and Nanotechnology Conference (MNC2022)
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of Through-Hole Type Nanoblade Array for Single Cell Dissection2022

    • Author(s)
      K. Ninomiya, R. Inoue, H. Takao, F. Shimokawa, K. Terao
    • Organizer
      35th International Microprocesses and Nanotechnology Conference (MNC2022)
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Three-dimensional nanofabrication with UV lithography of SU-8 for optically-driven microtools2022

    • Author(s)
      K. Tano, M. Harada, H. Takao, F. Shimokawa, K. Terao
    • Organizer
      35th International Microprocesses and Nanotechnology Conference (MNC2022)
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microfluidic Device for Parallel Chemical Stimulation to Local Area of Single Cell Surfaces2022

    • Author(s)
      R. Nakamura, H. Takao, F. Shimokawa, K. Hirano, K. Terao
    • Organizer
      35th International Microprocesses and Nanotechnology Conference (MNC2022)
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microfluidic device for local differentiation induction of iPS cells-derived embryoid bodies2022

    • Author(s)
      N. Kusunoki, S. Konagaya, M. Nishida, S. Sato, H. Takao, F. Shimokawa, K. Terao
    • Organizer
      26th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2022)
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Facile Fabrication of Two-Dimentional Micronozzle Array Using Skew-Positioned Wires as a Mold2022

    • Author(s)
      K. Takahashi, K. Terao
    • Organizer
      26th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2022)
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] iPS細胞胚様体の局所刺激による分化誘導を目指したマイクロデバイスの開発2022

    • Author(s)
      楠直人, 小長谷周平, 豊田太郎, 西田光徳, 佐藤成弘, 高尾英邦, 下川房男, 寺尾京平
    • Organizer
      第45回日本分子生物学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 敗血症の病態解明を目指した腎微小環境解析マイクロ流体実験システムの開発2022

    • Author(s)
      荒木佑弥, 小山哲哉, 高尾秀邦, 下川房男, 中野大介, 寺尾京平
    • Organizer
      第45回日本分子生物学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 血中がん細胞解析に向けた3次元毛細血管狭窄部モデルの開発2022

    • Author(s)
      青野航汰, 白神吉洋, 高尾英邦, 下川房男, 寺尾京平
    • Organizer
      第45回日本分子生物学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 抗体修飾光駆動ツールとマイクロ流体デバイスを用いた1 細胞組立プラットフォームの開発2022

    • Author(s)
      伊藤拓海, 森駿太郎, 高尾英邦, 下川房男, 寺尾京平
    • Organizer
      化学とマイクロナノシステム学会第46回研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] マイクロ流路を利用した薬剤応答面積依存性の定量解析法の開発2022

    • Author(s)
      中村瑠太, 高尾英邦, 下川房男, 平野勝也, 寺尾京平
    • Organizer
      化学とマイクロナノシステム学会第46回研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] iPS 細胞胚様体内の細胞間相互作用評価に向けた局所薬剤刺激デバイスの開発2022

    • Author(s)
      楠直人, 小長谷周平, 豊田太郎, 西田光徳, 佐藤成弘, 高尾英邦, 下川房男, 寺尾京平
    • Organizer
      化学とマイクロナノシステム学会第46回研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 血中がん細胞動態解析に向けた3 次元毛細血管狭窄部モデルの開発2022

    • Author(s)
      青野航汰, 白神吉洋, 高尾英邦, 下川房男, 寺尾京平
    • Organizer
      化学とマイクロナノシステム学会第46回研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] SU-8 ナノ加工を用いた単一細胞情報取得のための超微細構造物の開発2022

    • Author(s)
      多納圭祐, 原田雅彦, 高尾英邦, 下川房男, 寺尾京平
    • Organizer
      化学とマイクロナノシステム学会第46回研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 敗血症の病態解明を目指した腎微小環境解析マイクロ流体実験システムの開発2022

    • Author(s)
      荒木佑弥, 小山哲哉, 高尾秀邦, 下川房男, 中野大介, 寺尾京平
    • Organizer
      化学とマイクロナノシステム学会第46回研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] iPS 細胞の効率的分化誘導を目指した微小溶液環境可変デバイスの開発2022

    • Author(s)
      深井大暉, 米田将太, 高尾英邦, 下川房男, 寺尾京平
    • Organizer
      化学とマイクロナノシステム学会第46回研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 細胞分割のための貫通孔型ナノブレードアレイの開発2022

    • Author(s)
      仁宮滉太, 井上遼太郎, 高尾英邦, 下川房男, 寺尾京平
    • Organizer
      令和4 年度電気・電子・情報関係学会四国支部連合大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] マイクロ流路と接続したin vitro 微小血管構造の形成2022

    • Author(s)
      伊藤義純, 高尾英邦, 下川房男, 寺尾京平
    • Organizer
      令和4 年度電気・電子・情報関係学会四国支部連合大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] On Site Cell Assembly Using Optically-Driven Microtools With Antibodyimmobilized Surface2021

    • Author(s)
      S.Mori, H.Takao, F.Shimokawa, K.Terao
    • Organizer
      25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Two-Dimentional Microaperture Array for On-Demand Formation of Heterogeneous Gel Fibers2021

    • Author(s)
      K.Takahashi,H.Takao,F.Shimokawa,K.Terao
    • Organizer
      25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 余剰受容体の定量解析用マイクロ流路デバイス2021

    • Author(s)
      中村瑠太、高尾英邦、下川房男、平野勝也、寺尾京平
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Siブレードアレイデバイスを用いた固定組織切片の分画回収技術の開発2021

    • Author(s)
      植田光貴、塩見太郎、高尾英邦、下川房男、三浦史仁、寺尾京平
    • Organizer
      第38回「センサ・マイクロマシンと応用システム」シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] 抗体修飾光駆動構造体とマイクロ流体デバイスを用いた1細胞組立プラットフォームの開発2020

    • Author(s)
      森駿太郎、高尾英邦、下川房男、寺尾京平
    • Organizer
      第37回「センサ・マイクロマシンと応用システム」シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] SU-8ナノ加工を利用した光操作型ナノニードルの開発2020

    • Author(s)
      原田雅彦、高尾英邦、下川房男、寺尾京平
    • Organizer
      第37回「センサ・マイクロマシンと応用システム」シンポジウム
    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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