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Development and application of novel tools for pathophysiological investigation of the brain by an alkyne-tagging approach

Research Project

Project/Area Number 20H02881
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 37030:Chemical biology-related
Research InstitutionKeio University

Principal Investigator

Nuriya Mutsuo  慶應義塾大学, 医学部(信濃町), 准教授 (60453544)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2023: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Keywordsアルキンタグ / 脳 / プローブ / 生理活性物質
Outline of Research at the Start

脳の中の細胞間の情報伝達は、私たちの脳、そして精神機能を支えています。そしてその不調は様々な脳・精神に関する疾患の原因となると考えられます。しかし、これらの情報伝達は、技術的な制約から、見ること、そしてそれにより解析することが困難でした。本研究は、このような情報伝達とその変化を、化学と生物学の融合であるケミカルバイオロジーの新たな手法を駆使することで可視化し、脳・精神機能の健康と病気の理解を深めることを目指します。

Outline of Final Research Achievements

The intercellular communication within the brain underlies our cognitive and mental functions, and its dysfunction is considered to cause various neurological and psychiatric disorders. However, due to technical constraints, observing and analyzing such communications has been challenging. In this study, we successfully developed a novel labeling method based on the fusion of chemistry and biology, known as chemical biology, to visualize the substances responsible for chemical communications. By using this method, we gained new insights into the detailed properties of intercellular communications including their sites of actions.

Academic Significance and Societal Importance of the Research Achievements

本研究成果の意義は、手法の開発とその応用の両面にある。まず、手法の面では、これまで重要性が理解されながら可視化解析ができなかったサイズの小さな生理活性物質の可視化を実現するアルキンタグという新たな簡便な手法を提供した。これにより、今後の生理活性物質の研究の発展に貢献することができると期待される。次に、開発に成功したドーパミンとオキシトシンの可視化ツールについては、それを利用することで脳内での作用に関しての新たな生物学的知見をもたらすのみならず、今後、薬剤の開発などに広く応用できるツールを提供することとなった。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (25 results)

All 2023 2022 2021 2020 Other

All Journal Article (12 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 4 results) Presentation (11 results) (of which Int'l Joint Research: 2 results,  Invited: 8 results) Remarks (2 results)

  • [Journal Article] 総説 オキシトシン可視化手法の開発と動態解析-オキシトシンの「見える化」とそこから見えてきたこと2023

    • Author(s)
      塗谷睦生
    • Journal Title

      BRAIN and NERVE

      Volume: 75 Issue: 8 Pages: 957-963

    • DOI

      10.11477/mf.1416202450

    • ISSN
      1344-8129, 1881-6096
    • Year and Date
      2023-08-01
    • Related Report
      2023 Annual Research Report
  • [Journal Article] Stimulated Raman scattering microscopy reveals a unique and steady nature of brain water dynamics2023

    • Author(s)
      Takanori Shinotsuka, Tsuyoshi Miyazawa, Keiko Karasawa, Yasuyuki Ozeki, Masato Yasui, Mutsuo Nuriya
    • Journal Title

      Cell Reports Methods

      Volume: 3 Issue: 7 Pages: 100519-100519

    • DOI

      10.1016/j.crmeth.2023.100519

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 愛情の可視化へ:オキシトシンの脳内分布や動きを明かす2023

    • Author(s)
      中村花穂、塗谷睦生
    • Journal Title

      現代化学

      Volume: 626 Pages: 42-46

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Protocol to image deuterated propofol in living rat neurons using multimodal stimulated Raman scattering microscopy2023

    • Author(s)
      Zhong W, Oda R, Ozeki Y, Yasui M, Nuriya M
    • Journal Title

      STAR Protocols

      Volume: 4(2) Issue: 2 Pages: 102221-102221

    • DOI

      10.1016/j.xpro.2023.102221

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nonlinear Microscopy Imaging of Living Cells2023

    • Author(s)
      塗谷睦生
    • Journal Title

      Seibutsu Butsuri

      Volume: 63 Issue: 1 Pages: 33-37

    • DOI

      10.2142/biophys.63.33

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 非線形光学顕微鏡の脳科学研究への応用2023

    • Author(s)
      塗谷睦生
    • Journal Title

      フォトニクスニュース

      Volume: 8(3) Pages: 131-135

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Probing the spatiotemporal dynamics of oxytocin in brain tissue using a simple peptide alkyne-tagging approach2022

    • Author(s)
      Nakamura K, Karasawa K, Yasui M, Nuriya M
    • Journal Title

      Analytical Chemistry

      Volume: 94(35) Issue: 35 Pages: 11990-11998

    • DOI

      10.1021/acs.analchem.2c00452

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of non-linear Raman scattering microscopy to pharmacology to visualize invisible targets2022

    • Author(s)
      塗谷睦生
    • Journal Title

      Folia Pharmacologica Japonica

      Volume: 157 Issue: 5 Pages: 371-375

    • DOI

      10.1254/fpj.22060

    • ISSN
      0015-5691, 1347-8397
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ラマン散乱を用いた細胞水イメージング2022

    • Author(s)
      塗谷睦生
    • Journal Title

      月刊「細胞」

      Volume: 54(13) Pages: 8-11

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct visualization of general anesthetic propofol on neurons by stimulated Raman scattering microscopy2022

    • Author(s)
      Robert Oda, Jingwen Shou, Wenying Zhong, Yasuyuki Ozeki, Masato Yasui, Mutsuo Nuriya
    • Journal Title

      iScience

      Volume: 25(3) Issue: 3 Pages: 103936-103936

    • DOI

      10.1016/j.isci.2022.103936

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Alkyne-Tagged Dopamines as Versatile Analogue Probes for Dopaminergic System Analysis2021

    • Author(s)
      Mutsuo Nuriya, Yosuke Ashikari, Takanori Iino, Takuya Asai, Jingwen Shou, Keiko Karasawa, Kaho Nakamura, Yasuyuki Ozeki, Yukari Fujimoto, Masato Yasui
    • Journal Title

      Analytical Chemistry

      Volume: 93(27) Issue: 27 Pages: 9345-9355

    • DOI

      10.1021/acs.analchem.0c05403

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Differentially regulated pools of aquaporin-4 (AQP4) proteins in the cerebral cortex revealed by biochemical fractionation analyses2021

    • Author(s)
      Julia Ramadhanti, Tomoko Yamada, Masato Yasui, Mutsuo Nuriya
    • Journal Title

      Journal of Pharmacological Sciences

      Volume: 146(1) Issue: 1 Pages: 58-64

    • DOI

      10.1016/j.jphs.2021.03.003

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 非線形光学と非蛍光標識を用いた生理活性物質の可視化解析2023

    • Author(s)
      塗谷睦生
    • Organizer
      東京大学IRCN光学セミナー
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 非線形光学顕微鏡を用いた細胞・組織内分子動態の解析2022

    • Author(s)
      塗谷睦生
    • Organizer
      超高速光エレクトロニクス研究会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] マルチモダル多光子顕微鏡を用いた生理活性物質の可視化解析2022

    • Author(s)
      塗谷睦生
    • Organizer
      2021年度日本分光学会生細胞分光部会研究会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 微視的薬物動態の解析手段としての非線形光学顕微鏡2022

    • Author(s)
      塗谷睦生
    • Organizer
      第95回日本薬理学会年会
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 非線形光学イメージングの生命科学研究への応用2022

    • Author(s)
      塗谷睦生
    • Organizer
      第42回レーザー学会学術講演会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 脳組織内オキシトシンの動態解析を実現する新規プローブの開発と応用2021

    • Author(s)
      中村花穂、塗谷睦生
    • Organizer
      第145回日本薬理学会関東部会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 新規プローブの開発と応用による低分子量生体分子の可視化解析2021

    • Author(s)
      塗谷睦生
    • Organizer
      第30回日本バイオイメージング学会学術集会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] アルキンタグを用いた低分子量生理活性物質の新たな可視化法の開発と応用2021

    • Author(s)
      塗谷睦生
    • Organizer
      4大学医工連携セミナー ~光×超音波×近赤外蛍光による医工連携イメージングと健康長寿への道~
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] High-speed super-multiplex imaging of brain tissue2021

    • Author(s)
      Robert Oda, Jingwen Shou, Keiko Karasawa, Mutsuo Nuriya, Masato Yasui, Yasuyuki Ozeki
    • Organizer
      SPIE BiOS, 2021
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 非線形光学顕微鏡 ~SHG 顕微鏡~2020

    • Author(s)
      塗谷睦生
    • Organizer
      日本分光学会 第56 回 秋期セミナー
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 多光子現象を駆使した脳内化学情報伝達の可視化解析2020

    • Author(s)
      塗谷睦生
    • Organizer
      学術変革(B)革新ラマン領域会議
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Remarks] 塗谷グループのホームページ

    • URL

      http://user.keio.ac.jp/~aa606547/homepage.html

    • Related Report
      2023 Annual Research Report 2021 Annual Research Report 2020 Annual Research Report
  • [Remarks] 塗谷グループホームページ

    • URL

      http://user.keio.ac.jp/~aa606547/homepage.html

    • Related Report
      2022 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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