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Elucidation of the host-mediated selection mechanism for rhizobia after intracellular symbiosis establishment and evaluation of its impact on the soil rhizobial community

Research Project

Project/Area Number 20H02884
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionTohoku University

Principal Investigator

Sato Shusei  東北大学, 生命科学研究科, 教授 (70370921)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2022: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2021: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2020: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywords宿主-根粒菌相互作用 / Cheating根粒菌 / 宿主制裁機構 / 溶菌制御 / G x G 相互作用 / 水平伝搬 / 窒素固定活性 / 共生アイランド水平伝搬 / 植物-根粒菌相互作用 / 共生菌選抜機構 / 細胞内共生
Outline of Research at the Start

代表的な相利共生であるマメ科植物と根粒菌の共生において、共生相手の選別は重要な意味を持つ。この選別機構について、細胞内共生に至るまでの過程は詳細な解析が進められてきたが、細胞内共生後の選別機構については未解明の部分が多く残されている。本研究では、先行研究で明らかにした排除レベルの異なる宿主系統と根粒菌株の組み合わせを用いて、大規模な遺伝子発現解析を行うことにより、細胞内共生成立後の根粒菌選別機構を明らかにするとともに、この選別機構が共生後の土壌の根粒菌集団の構造に与える影響を調査する。これにより、マメ科植物による根粒菌の菌叢コントロールについて新しい概念を提示することを目指す。

Outline of Final Research Achievements

The host sanction system against cheating rhizobia (infectious but non-nitrogen fixing rhizobia) is the crucial mechanisms for maintaining symbiotic relationships. In this study, we investigated the molecular mechanisms of host sanction system by using its mutant isolated in L. japonicus and filed isolated cheating rhizobia with different levels of nitrogen fixation. As a result, we revealed that the sanctions against cheating rhizobia are mediated through the control of membrane mediated rhizobial lysis levels corresponding to their nitrogen-fixing abilities. Additionally, we found that the causal gene of the mutant is widely conserved in angiosperms, and their expression levels decrease during symbiosis with functional rhizobia.

Academic Significance and Societal Importance of the Research Achievements

本研究により、これまで不明であった根粒特異的な抗菌ペプチドを持たないミヤコグサにおけるcheating根粒菌に対する制裁機構が、マメ科植物に特異的なものではなく、被子植物に広く保存されている防御反応を応用している可能性が示された。今後この遺伝子の機能解析や共生過程で制御機構の解析を進めることにより、細胞内共生の成立起源につながるような知見が得られることが期待される。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (17 results)

All 2022 2021 2020 Other

All Int'l Joint Research (4 results) Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 6 results) Presentation (7 results) (of which Invited: 2 results)

  • [Int'l Joint Research] Aarhus University(デンマーク)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Suranaree University of Technology(タイ)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Aarhus University(デンマーク)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Aarhus University(デンマーク)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Phenolic Acids Induce Nod Factor Production in <i>Lotus japonicus</i>–<i>Mesorhizobium</i> Symbiosis2022

    • Author(s)
      Shimamura Masayuki、Kumaki Takashi、Hashimoto Shun、Saeki Kazuhiko、Ayabe Shin-ichi、Higashitani Atsushi、Akashi Tomoyoshi、Sato Shusei、Aoki Toshio
    • Journal Title

      Microbes and Environments

      Volume: 37 Issue: 1 Pages: n/a

    • DOI

      10.1264/jsme2.ME21094

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Widespread and transgenerational retrotransposon activation in inter‐ and intraspecies recombinant inbred populations of <i>Lotus japonicus</i>2022

    • Author(s)
      Fukai Eigo、Yoshikawa Manabu、Shah Niraj、Sandal Niels、Miyao Akio、Ono Seijiro、Hirakawa Hideki、Akyol Turgut Yigit、Umehara Yosuke、Nonomura Ken‐Ichi、Stougaard Jens、Hirochika Hirohiko、Hayashi Makoto、Sato Shusei、Andersen Stig Uggerh?j、Okazaki Keiichi
    • Journal Title

      The Plant Journal

      Volume: 111 Issue: 5 Pages: 1397-1410

    • DOI

      10.1111/tpj.15896

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] <i>FER</i> and <i>LecRK</i> show haplotype-dependent cold-responsiveness and mediate freezing tolerance in <i>Lotus japonicus</i>2022

    • Author(s)
      Mustamin Yusdar、Akyol Turgut Yigit、Gordon Max、Manggabarani Andi Madihah、Isomura Yoshiko、Kawamura Yasuko、Bamba Masaru、Williams Cranos、Andersen Stig Uggerh?j、Sato Shusei
    • Journal Title

      Plant Physiology

      Volume: 191 Issue: 2 Pages: 1138-1152

    • DOI

      10.1093/plphys/kiac533

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evolution of rhizobial symbiosis islands through insertion sequence-mediated deletion and duplication2021

    • Author(s)
      Arashida Haruka、Odake Haruka、Sugawara Masayuki、Noda Ryota、Kakizaki Kaori、Ohkubo Satoshi、Mitsui Hisayuki、Sato Shusei、Minamisawa Kiwamu
    • Journal Title

      The ISME Journal

      Volume: 16 Issue: 1 Pages: 112-121

    • DOI

      10.1038/s41396-021-01035-4

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Insights into the evolution of symbiosis gene copy number and distribution from a chromosome-scale Lotus japonicus Gifu genome sequence2020

    • Author(s)
      Kamal Nadia、Mun Terry、Reid Dugald、Lin Jie-Shun、Akyol Turgut Yigit、Sandal Niels、Asp Torben、Hirakawa Hideki、Stougaard Jens、Mayer Klaus F X、Sato Shusei、Andersen Stig Uggerh?j
    • Journal Title

      DNA Research

      Volume: 27 Issue: 3

    • DOI

      10.1093/dnares/dsaa015

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Massive rhizobial genomic variations associated with partner quality in Lotus-Mesorhizobium symbiosis2020

    • Author(s)
      Masaru Bamba, Seishiro Aoki, Tadashi Kajita, Hiroaki Setoguchi, Yasuyuki Watano, Shusei Sato, Takashi Tsuchimatsu
    • Journal Title

      FEMS Microbiology Ecology

      Volume: 96 Issue: 12

    • DOI

      10.1093/femsec/fiaa202

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 日本のマメ科遺伝資源としてのミヤコグサ・ダイズ2022

    • Author(s)
      佐藤 修正
    • Organizer
      日本育種学会・令和4年度秋季大会シンポジウム
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 細胞内共生成立後の根粒菌選別機構に関わるミヤコグサPINK4遺伝子の機能解析2022

    • Author(s)
      嵐田遥, 中川知巳, 眞板寛子, 日下部翔平, 番場大, Yusdar Mustamin, 佐藤修正
    • Organizer
      植物微生物研究会 第31回研究交流会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Functional analysis of PINK4 gene of Lotus japonicus in symbiont selection after the establishment of endosymbiosis.2022

    • Author(s)
      Haruka Arashida, Tomomi Nakagawa, Hiroko Maita, Shohei Kusakabe, Shusei Sato
    • Organizer
      第63回 日本植物生理学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ミヤコグサリソースを活用した環境適応機構の解析2022

    • Author(s)
      佐藤 修正, Yusdar Mustamin, 番場 大, Turgut Akyol, Stig Andersen, 橋口 正嗣, 橋口 拓勇, 田中 秀典, 明石 良
    • Organizer
      第63回 日本植物生理学会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 細胞内共生成立後の根粒菌選別機構に関わるミヤコグサPINK4遺伝子の機能解析2021

    • Author(s)
      嵐田遥, 中川知巳, 眞板寛子, 日下部翔平, 佐藤修正
    • Organizer
      植物微生物研究会 第30回研究交流会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ミヤコグサ関連リソースの最新状況とその応用2021

    • Author(s)
      佐藤 修正, , Yusdar Mustamin, Madihah Manggabarani, 番場 大, Turgut Akyol, Stig Andersen, Nadia Kamal, Klaus Mayer, 橋口 正嗣, 田中 秀典, 明石 良
    • Organizer
      第62回日本植物生理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] マメ科植物-根粒菌共生関係における遺伝子型x遺伝子型(GxG)相互作用に関連する植物遺伝子座に働く自然選択2020

    • Author(s)
      番場 大、青木 誠志朗、梶田 忠、瀬戸口 浩章、綿野 泰行、佐藤 修正、土松 隆志
    • Organizer
      日本共生生物学会(Symbio2020)
    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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