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Elucidation of branched-electron transport chain in iodate-respiring bacteria

Research Project

Project/Area Number 20H02896
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionChiba University

Principal Investigator

Amachi Seigo  千葉大学, 大学院園芸学研究院, 教授 (80323393)

Co-Investigator(Kenkyū-buntansha) 山村 茂樹  国立研究開発法人国立環境研究所, 地域環境保全領域, 室長 (90414391)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2022: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2021: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2020: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywordsヨウ素酸呼吸 / 電子伝達鎖 / idr遺伝子 / 放射性ヨウ素 / 地下水汚染 / 銀担持ゼオライト / バイオレメディエーション / Idr / Pseudomonas sp. SCT / IdrABCD / ヨウ素 / Pseudomonas / ヘムタンパク
Outline of Research at the Start

ヨウ素酸(IO3-)呼吸細菌は、ヒトの必須元素ヨウ素の地球規模での循環に重要と考えられ、放射性ヨウ素129I汚染環境の修復への応用も期待される。これまでの研究から、ヨウ素酸呼吸には新規金属タンパク質複合体Idrが関与すること、また電子伝達鎖が3つに分岐するという、珍しい特徴を持つことがわかってきた。そこで本研究では、複数の遺伝子破壊株の作成により、ヨウ素酸呼吸に関わるタンパク質と電子伝達鎖の全容を解明する。また、核施設周辺で問題となっている地下水の129I汚染の修復を目標に、SCTを用いた模擬地下水からの129Iの回収・除去試験を行う。

Outline of Final Research Achievements

Several types of gene knockout mutants were constructed from an iodate-respiring bacterium Pseudomonas sp. SCT, and their growth under iodate-respring, denitrifying, and oxygen-respiring conditions was compared with that of the wild-type strain. By using one of these mutants, effect of iodate and iodide concentrations on the transcription of idr gene was determined. Idr protein complex was purified, and its molecular weight and subunit composition were deduced. Furthermore, silver-impregnated zeolite was added to the spent medium of Azoarcus sp. DN11, another iodate-respiring bacterium isolated from gasoline-contaminated subsurface aquifer. Iodine was successfully removed from the aqueous phase and recovered as silver iodide (AgI). These results suggest that iodate-respiring bacteria are potentially helpful for bioaugmentation of radioiodine (I-129)-contaminated subsurface aquifers.

Academic Significance and Societal Importance of the Research Achievements

ヨウ素酸呼吸は最近になって発見された細菌の嫌気呼吸様式で、脊椎動物の必須元素であるヨウ素の地球規模での循環に与える影響から注目を集めている。またヨウ素酸呼吸細菌は、地下水汚染が報告されている放射性ヨウ素(I-129)の回収・除去にも応用が期待される。本研究では2種類のヨウ素呼吸細菌を用いて、ヨウ素酸呼吸に重要な役割を演じる複数の遺伝子を同定し、その機能を予測した。また、ヨウ素酸呼吸反応を触媒するタンパク質の分子量とサブユニット構造を予測した。さらに、ヨウ素酸呼吸細菌を用いてヨウ素酸をヨウ化物に変換することで、I-129をヨウ化銀(AgI)として効率的に回収・除去できることを示すデータを得た。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (13 results)

All 2023 2022 2021 2020

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (8 results) (of which Invited: 1 results)

  • [Journal Article] Iodate respiration by Azoarcus sp. DN11 and its potential use for removal of radioiodine from contaminated aquifers2023

    • Author(s)
      Sasamura Seiya、Ohnuki Toshihiko、Kozai Naofumi、Amachi Seigo
    • Journal Title

      Frontiers in Microbiology

      Volume: 14

    • DOI

      10.3389/fmicb.2023.1162788

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Decolorization of cationic dyes under alkaline conditions by Iodidimonas sp. Q-1 multicopper oxidase2022

    • Author(s)
      Ebihara Kyota、Yoshikawa Jun、Horiguchi Hirofumi、Amachi Seigo
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 133 Issue: 4 Pages: 323-328

    • DOI

      10.1016/j.jbiosc.2022.01.007

    • NAID

      210000159604

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Iodidimonas gelatinilytica sp. nov., aerobic iodide-oxidizing bacteria isolated from brine water and surface seawater2021

    • Author(s)
      Iino Takao、Oshima Kenshiro、Hattori Masahira、Ohkuma Moriya、Amachi Seigo
    • Journal Title

      Antonie van Leeuwenhoek

      Volume: 114 Issue: 5 Pages: 625-631

    • DOI

      10.1007/s10482-021-01546-2

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reductive deiodination of 2,4,6-triiodophenol by Vallitalea sp. strain TIP-1 isolated from the marine sponge2021

    • Author(s)
      Kawamura Yuki、Mori Koji、Amachi Seigo
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 132 Issue: 2 Pages: 154-160

    • DOI

      10.1016/j.jbiosc.2021.04.011

    • NAID

      40022657711

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel dimethylsulfoxide reductase family of molybdenum enzyme, Idr, is involved in iodate respiration by Pseudomonas sp. SCT2020

    • Author(s)
      Yamazaki Chihiro、Kashiwa Sumie、Horiuchi Ayaka、Kasahara Yasuhiro、Yamamura Shigeki、Amachi Seigo
    • Journal Title

      Environmental Microbiology

      Volume: - Issue: 6 Pages: 2196-2212

    • DOI

      10.1111/1462-2920.14988

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] 海洋好気性細菌によるヨウ素酸還元2022

    • Author(s)
      木根健、天知誠吾
    • Organizer
      日本農芸化学会関東支部2022年度大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] idrAとidrPはPseudomonas sp. SCTのヨウ素酸呼吸に必須である2022

    • Author(s)
      柏澄江、香取七奈、黒田真史、天知誠吾
    • Organizer
      日本農芸化学会関東支部2022年度大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Pseudomonas sp. SCT株の異化的ヨウ素酸還元酵素Idrの精製2022

    • Author(s)
      久保拓舞、天知誠吾
    • Organizer
      第20回微生物研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 海洋好気性細菌によるヨウ素酸還元2022

    • Author(s)
      木根健、天知誠吾
    • Organizer
      第20回微生物研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Pseudomonas sp. SCT株におけるidr遺伝子群の破壊がヨウ素酸呼吸に与える影響2022

    • Author(s)
      柏澄江、香取七奈、黒田真史、天知誠吾
    • Organizer
      日本微生物生態学会第34回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 微生物の金属タンパクと元素循環:ヨウ素、ヒ素、アンチモンを例として2022

    • Author(s)
      天知誠吾
    • Organizer
      立命館大学「生命×金属」シンポジウム(オンライン開催)
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] idrAとidrPはPseudomonas sp. SCT株のヨウ素酸呼吸に必須である2021

    • Author(s)
      柏澄江、黒田真史、天知誠吾
    • Organizer
      日本微生物生態学会第34回大会(オンライン開催)
    • Related Report
      2021 Annual Research Report
  • [Presentation] 細菌のヨウ素酸呼吸には新規鉄硫黄モリブデンタンパクIdrが関与する2020

    • Author(s)
      柏澄江、山﨑千尋、笠原康裕、天知誠吾
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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