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Control of root-associated microbiome by a beneficial fungus for plant growth promotion

Research Project

Project/Area Number 20H02986
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionThe University of Tokyo

Principal Investigator

Hiruma Kei  東京大学, 大学院総合文化研究科, 准教授 (20714504)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2022: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2021: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords内生糸状菌 / 病原菌 / シロイヌナズナ / 貧栄養応答 / 病原細菌 / 揮発性物質 / 病原性抑制 / 栄養 / Colletotrichum / Pseudomonas / 植物生長促進 / 貧栄養 / 植物保護 / 共生菌 / 微生物叢 / 遺伝子発現 / 窒素枯渇 / リン枯渇 / マイクロバイオーム / 共生糸状菌 / 植物栄養 / 栄養枯渇 / アブラナ科植物 / 圃場
Outline of Research at the Start

植物は、多様な微生物叢と相互作用しており、これらの微生物叢は植物の環境適応に必要不可欠な役割を担っていると考えられている。本研究では、アブラナ科植物の根に共生する糸状菌が窒素欠乏環境で特定の細菌群を根圏に誘引し、それらと協調して植物生長を促す仕組みを理解すること、および、それら根圏細菌群が有する潜在的な寄生性を共生糸状菌が抑止する仕組みを実験室および野外圃場環境で理解することを目的とする。以上から、複数種類の微生物と植物からなる複雑な相互作用機作を理解することに加えて、複数の微生物を総体として活用することで、有用微生物資材を野外環境でも安定的に発揮させることを可能とする方法論につなげたい。

Outline of Final Research Achievements

The root endophytic fungus Colletotrichum tofieldia (Ct) promotes plant growth under low phosphorus (Pi) conditions and induces alterations in the root bacterial community composition. In this investigation, we specifically examine a pathogenic bacterium whose relative abundance is enhanced in the presence of Ct. Our findings reveal that unidentified volatile compounds emitted by the bacteria are responsible for inhibiting plant growth. Notably, the presence of Ct substantially mitigates this growth inhibition. Moreover, we observe that bacterial-derived volatiles even stimulate plant growth under low Pi conditions when the bacteria are cultivated in nutrient-depleted environments. Hence, we ascertain that the production of these bacteria-derived volatiles is significantly influenced by the nutrient status, thereby exerting a context-dependent effect on plant growth, transitioning from negative to positive.

Academic Significance and Societal Importance of the Research Achievements

植物の成長に有益な共生糸状菌を理解することは、共生糸状菌を活用した農業技術を開発していくためにも重要です。本研究では、共生糸状菌による病原菌抑制機構を理解する一歩として、共生糸状菌が根に誘引する病原細菌に着目して進めたところ、病原細菌由来の揮発性物質が病原性発現に重要であること、および、その病原性は共生糸状菌によって弱められることが判明しました。さらには、病原細菌を貧栄養環境下で生育させた際には、細菌から放出される揮発性物質が植物成長を促すことも発見しました。したがって、本病原細菌は栄養環境に応じて植物成長にプラスからマイナスと対照的な影響を与える揮発性物質を放出していることが考えられました。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (18 results)

All 2023 2022 2021 2020 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (10 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Genome Resource of <i>Colletotrichum spaethianum</i>, the Causal Agent of Leaf Anthracnose in <i>Polygonatum falcatum</i>2022

    • Author(s)
      Utami Yuniar Devi、Hiruma Kei
    • Journal Title

      PhytoFrontiers

      Volume: 2 Issue: 2 Pages: 152-155

    • DOI

      10.1094/phytofr-12-21-0082-a

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Investigating plant?microbe interactions within the root2022

    • Author(s)
      Utami Yuniar Devi、Nguyen Tan Anh Nhi、Hiruma Kei
    • Journal Title

      Archives of Microbiology

      Volume: 204 Issue: 10

    • DOI

      10.1007/s00203-022-03257-2

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dysbiosis of the rhizosphere microbiome caused by γ-irradiation alters the composition of root exudates and reduces phosphorus uptake by rice in flooded soils2022

    • Author(s)
      Mukai Mana、Hiruma Kei、Nishigaki Tomohiro、Utami Yuniar Devi、Otaka Junnosuke、Yoshihashi Tadashi、Sarr Papa Saliou、Oo Aung Zaw、Takai Toshiyuki、Tujimoto Yasuhiro
    • Journal Title

      Plant and Soil

      Volume: . Issue: 1-2 Pages: 1-17

    • DOI

      10.1007/s11104-022-05726-5

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Continuous Spectrum of Lifestyles of Plant-Associated Fungi Under Fluctuating Environments: What Genetic Components Determine the Lifestyle Transition?2020

    • Author(s)
      Hiruma Kei
    • Journal Title

      Evolutionary Biology―A Transdisciplinary Approach. Springer, Cham.

      Volume: - Pages: 117-132

    • DOI

      10.1007/978-3-030-57246-4_6

    • ISBN
      9783030572457, 9783030572464
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] Plant tryptophan metabolism pathway is crucial for plant growth promotion by Colletotrichum tofieldiae under nitrogen deficiency2023

    • Author(s)
      Tan Anh Nhi Nguyen, Masami Nakamura, Kei Hiruma
    • Organizer
      5年度日本植物病理学会全国大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Nutrient status determines Psudomonas aeruginosa effect on Arabidopsis thaliana growth2023

    • Author(s)
      Yuniar Devi Utami, Kei Hiruma
    • Organizer
      5年度日本植物病理学会全国大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Root endophyte Colletotrichum tofieldiaepromotes Arabidopsis thaliana growth under nitrogen limiting conditions2023

    • Author(s)
      Tan Anh Nhi Nguyen, Yuniar Devi Utami, Masami Nakamura, Kei Hiruma
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Root endophyte Colletotrichum tofieldiae promotes brassica plant growth and recruits beneficial bacteria to roots under nitrogen-limiting condit2022

    • Author(s)
      Tan Anh Nhi Nguyen, Yuniar Devi Utami, Masami Nakamura, Kei Hiruma
    • Organizer
      12th Japan-US Seminar in Plant Pathology
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Suppression of bacterial root growth inhibition by Colletotrichum tofieldiae in Arabidopsis thaliana2022

    • Author(s)
      Yuniar Devi Utami, Salvatore Cosentino, Anda Mizue, Iwasaki Wataru, Hiruma Kei
    • Organizer
      日本植物病理学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 植物共生糸状菌が秘める病原性の覚醒2021

    • Author(s)
      晝間敬
    • Organizer
      糸状菌分子生物学コンファレンス
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Roles of fungal secondary metabolism clusters during plant-microbe interactions2021

    • Author(s)
      晝間敬
    • Organizer
      日本生化学学会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 糸状菌・細菌共生集合体が栄養枯渇環境下での植物生長に果たす役割2021

    • Author(s)
      晝間敬
    • Organizer
      日本植物生理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 菌の植物ホルモン合成クラスターの活性化が植物共生糸状菌 Colletotrichum tofieldiae の寄生化を促す2021

    • Author(s)
      晝間敬、青木誠志郎、川邑菜々美、内山朱美、岩崎渉、西條雄介
    • Organizer
      日本植物病理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Molecular basis that induce lifestyle transitions in plant-associated endophytic fungi2020

    • Author(s)
      晝間敬
    • Organizer
      分子生物学会
    • Related Report
      2020 Annual Research Report
  • [Book] メタオミクス解析を活用した植物有用微生物の単離同定2022

    • Author(s)
      Yuniar Devi Utami, 晝間敬
    • Total Pages
      500
    • Publisher
      株式会社エヌ・ティー・エス
    • Related Report
      2021 Annual Research Report
  • [Remarks] 研空室ホームページ

    • URL

      https://park.itc.u-tokyo.ac.jp/hiruma/

    • Related Report
      2022 Annual Research Report
  • [Remarks] 東京大学晝間研究室

    • URL

      http://park.itc.u-tokyo.ac.jp/hiruma/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 晝間研究室ホームページ

    • URL

      http://park.itc.u-tokyo.ac.jp/hiruma/

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2020-04-28   Modified: 2024-01-30  

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