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The diversity of viral communities and their infection dynamics in the rhizosphere of crops

Research Project

Project/Area Number 20H02987
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionOkayama University

Principal Investigator

Kondo Hideki  岡山大学, 資源植物科学研究所, 准教授 (40263628)

Co-Investigator(Kenkyū-buntansha) 久野 裕  岡山大学, 資源植物科学研究所, 准教授 (70415454)
鈴木 信弘  岡山大学, 資源植物科学研究所, 教授 (70206514)
兵頭 究  岡山大学, 資源植物科学研究所, 准教授 (80757881)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2022: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2021: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2020: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywordsウイルス / 作物 / 根圏 / リザーバー / オオムギ / アブラムシ / うどんこ病 / 水平伝搬 / リザーバ
Outline of Research at the Start

作物の生育は根圏に生息する微生物や無脊椎動物により様々な影響を受けている。これら根圏の生物は多様なウイルスを保持していると想定されるが、その多様性やその作物生産へのインパクトは解明されていない。代表者らは、ウイルス病が慢性的に問題となるオオムギの根圏に存在するウイルスに注目し、①根圏ウイルス群とその宿主(菌類・昆虫を想定)探索、②根圏ウイルスの生物界を跨ぐ水平伝搬と宿主への影響、③根圏ウイルスの年次変動とムギ類ウイルスの病原性(再)評価を進める。以上、作物根圏ウイルスの多様性と感染動態の解析から作物生産への影響を理解し、得られた知見を基盤としたウイルス制御や利活用を目指している。

Outline of Final Research Achievements

Crops are affected by various microorganisms and invertebrates that inhabit the phyllosphere and rhizosphere. It is assumed that these organisms carry a wide range of viruses, but their diversity and impact on crop production have not been well understood. In this project, our focus was on barley and wheat crops, and we conducted in-depth studies of viromes in the rhizosphere (and also the phyllosphere), which had not been extensively analyzed before. By using RNA-seq analysis on field samples of barley and wheat over a three-year period, we discovered multiple RNA viruses that infect plants, as well as insects, including previously unknown viruses. Furthermore, we identified a wide range of mycovirus candidates in the rhizosphere, although their actual hosts are yet to be determined. These findings are expected to provide valuable insights into the diversity of viral communities in barley and wheat agroecosystems, and they will contribute to future studies on infectious dynamics.

Academic Significance and Societal Importance of the Research Achievements

本研究では、これまでほとんど理解されていなかったムギ類(オオムギとコムギ)生存圏のウイルス叢の理解に向けてRNA-seqを行い、多くのウイルス学的な知見を蓄積することができた。特に、特定圃場におけるウイルスリファレンス配列の取得(新規植物・昆虫ウイルスや多くの菌類ウイルス候補を含む)は、圃場レベルでのウイルス叢の動態解析や生態学的な役割を紐解くために活用できると期待される。将来的には、ムギ類ウイルスのリスク再評価とウイルス抵抗性育種への応用、ムギ類作物のバイオコントロールへ向けたウイルス資材の開発、持続的作物生産における菌類および菌類ウイルスの利用へ向けた基礎知見の蓄積につながる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (34 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (8 results) Journal Article (15 results) (of which Int'l Joint Research: 11 results,  Peer Reviewed: 15 results,  Open Access: 12 results) Presentation (6 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (4 results) Remarks (1 results)

  • [Int'l Joint Research] Qingdao Agricultural University/Northwest A&F University(中国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Swiss Federal Research Institute WSL(スイス)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Mendel University(チェコ)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] The University of Queensland(オーストラリア)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Qingdao Agricultural University/Northwest A&F University(中国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] The University of Queensland(オーストラリア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Northwest A&F University/Qingdao Agricultural University(中国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] The University of Queensland(オーストリア)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan2023

    • Author(s)
      Kondo H、Sugahara H、Fujita M、Hyodo K、Andika IB、Hisano H、Suzuki N.
    • Journal Title

      Pathogens

      Volume: 12 Issue: 3 Pages: 358-358

    • DOI

      10.3390/pathogens12030358

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Common but nonpersistent acquisitions of plant viruses by plant-associated fungi2022

    • Author(s)
      Cao X, Liu J, Pang J, Kondo H, Chi S, Zhang J, Sun L, Andika IB
    • Journal Title

      Viruses

      Volume: 14 Issue: 10 Pages: 2279-2279

    • DOI

      10.3390/v14102279

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nature cross-kingdom spred of apple scar skin viroid from apple plants to fungi2022

    • Author(s)
      Tian M, Wei S, Bian R, Luo J, Khan HA, Tai H, Kondo H, Hadidi A, Andika IB, Sun L
    • Journal Title

      Cells

      Volume: 11 Issue: 22 Pages: 3686-3686

    • DOI

      10.3390/cells11223686

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mycovirus diversity and evolution revealed/inferred from recent studies2022

    • Author(s)
      Kondo, H., Botella, L., Suzuki N.
    • Journal Title

      Annual Review of Phytopathology

      Volume: 60 Issue: 1 Pages: 307-336

    • DOI

      10.1146/annurev-phyto-021621-122122

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ICTV Virus Taxonomy Profile: Rhabdoviridae2022

    • Author(s)
      Walker PJ (Kondo, H) et a.l
    • Journal Title

      Journal of General Virology

      Volume: 103 Issue: 6 Pages: 001689-001689

    • DOI

      10.1099/jgv.0.001689

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of a novel quinvirus in family Betaflexiviridae that infects winter wheat2022

    • Author(s)
      Kondo, H., Yoshida, N., Fujita, M., Maruyama, K., Hyodo, K., Hiasno, H., Tamada, T., Andika, I.B. and Suzuki, N.
    • Journal Title

      Frontiers in Microbiology

      Volume: 12 Pages: 715545-715545

    • DOI

      10.3389/fmicb.2021.715545

    • NAID

      120007147386

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] First Report of Chinese wheat mosaic virus Infecting Barley in Japan2022

    • Author(s)
      Kondo Hideki、Masejima Hidekazu、Maruyama Kazuyuki、Fujita Miki、Ohki Takehiro
    • Journal Title

      Plant Disease

      Volume: in press Issue: 8 Pages: 2272-2272

    • DOI

      10.1094/pdis-12-21-2803-pdn

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel deltapartitivirus from red clover2022

    • Author(s)
      Telengech, P., Shahi, S., Kondo, H. and Suzuki, N.
    • Journal Title

      Archives of Virology

      Volume: 167 Issue: 4 Pages: 1201-1204

    • DOI

      10.1007/s00705-022-05372-3

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epidemic progress of beet necrotic yellow vein virus: evidence from an investigation in Japan spanning half a century2022

    • Author(s)
      Nakagami, R., Chiba, S., Yoshida, N., Senoo, Y., Iketani-Saito, M., Iketani, S., Kondo, H., and Tamada, T.
    • Journal Title

      Plant Pathology

      Volume: 71 Issue: 3 Pages: 715-728

    • DOI

      10.1111/ppa.13504

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plant Viruses Reported from Japan2022

    • Author(s)
      Fuji S, Mochizuki T, Okuda M, Tsuda S, Kagiwada S, Sekine, K, Ugaki M, Natsuaki K-T, Isogai M, Maoka T, Takeshita M, Yoshikawa M, Mise K, Sasaya T, Kondo H, Kubota K, Yamaji Y, Iwanami T, Ohshima K, Kobayashi K, Hataya T, Sano T, Suzuki N.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 88 Issue: 2 Pages: 105-127

    • DOI

      10.1007/s10327-022-01051-y

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ICTV Virus Taxonomy Profile: Nyamiviridae 20212021

    • Author(s)
      Dietzgen, R.G. , Jiang, D. Junglen, S., Kondo, H., Kuhn, J.H., and ICTV Report Consortium.
    • Journal Title

      Journal of General Virology

      Volume: 102 Issue: 11 Pages: 001681-001681

    • DOI

      10.1099/jgv.0.001681

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pathogenetic roles of beet necrotic yellow vein virus RNA5 in the exacerbation of symptoms and yield reduction, development of scab-like symptoms, and Rz-resistance breaking in sugar beet.2021

    • Author(s)
      Tamada, T., Uchino, H., Kusume, T., Iketaniaito, M., Chiba, S., Andika, I. B., Kondo, H.
    • Journal Title

      Plant Pathology

      Volume: 70 Issue: 1 Pages: 219-232

    • DOI

      10.1111/ppa.13266

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Virome Analysis of Aphid Populations That Infest the Barley Field: The Discovery of Two Novel Groups of Nege/Kita-Like Viruses and Other Novel RNA Viruses.2020

    • Author(s)
      Kondo H, Fujita M, Hisano H, Hyodo K, Andika IB, Suzuki N
    • Journal Title

      Front Microbiol.

      Volume: 11 Pages: 509-509

    • DOI

      10.3389/fmicb.2020.00509

    • NAID

      120006860105

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Diverse partitiviruses from the phytopathogenic fungus, Rosellinia necatrix.2020

    • Author(s)
      Telengech, P., Hisano, S., Micheni, C. M., Hyodo, K., Arjona-Lopez, J.M., Lopez-Herrera, C., Kanematsu, S., Kondo, H., and Suzuki, N.
    • Journal Title

      Frontiers in Microbiology

      Volume: 11 Pages: 1064-1064

    • DOI

      10.3389/fmicb.2020.01064

    • NAID

      120006877069

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Diversity and epidemiology of plant rhabdoviruses.2020

    • Author(s)
      Dietzgen, R. Bejerman, N.B., Goodin, M.M., Higgins, C.M., Huot, O. B. Kondo, H., Martin, L. M., Whitfield, A.E
    • Journal Title

      Virus Research

      Volume: 281 Pages: 197942-197942

    • DOI

      10.1016/j.virusres.2020.197942

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Cross-kingdom viral infection in agroecosystems.2023

    • Author(s)
      Kondo, H.
    • Organizer
      38th IPSR International Symposium and 14th Symposium on Plant Stress Sciences. Kurashiki, Japan
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Novel, diverse RNA viruses in the Japanese population of the ash dieback fungus, Hymenoscyphus fraxineus2022

    • Author(s)
      Shamsi, W., Kondo, H., Rigling, D., Prospero, S
    • Organizer
      5th mycovirus symposium, Gargnano, Italy, May 30-June 2, 2022.
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] コムギに感染するベータフレキシ科の新規RNAウイルス2022

    • Author(s)
      近藤秀樹・吉田直人・兵頭 究・久野 裕・玉田哲男・鈴木信弘
    • Organizer
      令和4年度 日本植物病理学会大会, 札幌(オンライン)
    • Related Report
      2021 Annual Research Report
  • [Presentation] Subcellular localization of carrot temperate virus 1 capsid protein in the nucleus and cytoplasm2021

    • Author(s)
      Telengech, P., Hyodo, K., Wang, W-Q., Natsuaki, T., Suzuki, N
    • Organizer
      The Annual Meeting of the Japanese Phytopathological Society
    • Related Report
      2021 Annual Research Report
  • [Presentation] Diverse partitiviruses from the phytopathogenic fungus, Rosellinia necatrix2021

    • Author(s)
      Telengech, P., Hisano, S., Micheni, C. M., Hyodo, K., Arjona-Lopez, J.M., Lopez-Herrera, C., Kanematsu, S., Kondo, H., and Suzuki, N.
    • Organizer
      The 40th Annual Meeting of the American Society for Virology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] オオムギ圃場に生息するアブラムシのウイルス叢解析2021

    • Author(s)
      近藤秀樹・藤田美貴・久野 裕・兵頭 究・鈴木信弘
    • Organizer
      令和3年度日本植物病理学会大会(島根・オンライン開催)
    • Related Report
      2020 Annual Research Report
  • [Book] The border between kitavirids and nege-like viruses: Tracking the evolutionary pace of plant- and arthropod-infecting viruses2022

    • Author(s)
      Ramos-Gonzlez, P.L., Kondo, H., Morozov, S., Vasilakis, N., Varsani, A., Cao, M. and Freitas-Astua, J.(Eds.)
    • Total Pages
      778
    • Publisher
      Frontiers in Plant Science (ISBN 978-2-88976-368-9)
    • Related Report
      2022 Annual Research Report
  • [Book] Cross-kingdom virus transmission (Encyclopedia of Virology. 4th edition)2021

    • Author(s)
      Sun L. Kondo, H., Andika, I.B.
    • Publisher
      Elsevier Ltd.
    • ISBN
      9780128145159
    • Related Report
      2021 Annual Research Report
  • [Book] Quadriviruses (Quadriviridae). Encyclopedia of Virology 4th Edition (vol 4, 642-647)2020

    • Author(s)
      Kondo, H., Caston, J.R., Suzuki, N.
    • Total Pages
      4103
    • Publisher
      Elsevier
    • ISBN
      9780128145159
    • Related Report
      2020 Annual Research Report
  • [Book] Cross-Kingdom Virus Infection. Encyclopedia of Virology 4th Edition (vol 4, 443-449)2020

    • Author(s)
      Sun, L., Kondo, H., Andika, I. B.
    • Total Pages
      4103
    • Publisher
      Elsevier
    • ISBN
      9780128145159
    • Related Report
      2020 Annual Research Report
  • [Remarks] 岡山大学資源植物科学研究所 植物・微生物相互作用グループ

    • URL

      http://www.rib.okayama-u.ac.jp/pmi/index-j.html

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report 2020 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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