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Integrated analysis of the complex proteomics and spectroscopic methods for the photosynthetic regulatory mechanisms in evergreen conifers.

Research Project

Project/Area Number 20H03017
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40010:Forest science-related
Research InstitutionHokkaido University

Principal Investigator

Tanaka Ryouichi  北海道大学, 低温科学研究所, 教授 (20311516)

Co-Investigator(Kenkyū-buntansha) 秋本 誠志  神戸大学, 理学研究科, 准教授 (40250477)
北尾 光俊  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (60353661)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2023: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords光合成 / 樹木 / 光化学系2 / 常緑針葉樹 / 熱放散
Outline of Research at the Start

寒冷域で常緑針葉樹が越冬するためには、細胞や組織が凍結耐性をもつだけでなく、光化学系が光障害を受けないことが重要である。常緑針葉樹は気温に応じて、99%の光エネルギーを放散することのできる可逆的な調節メカニズムを持ち、低温下での光障害を防いでいる。このメカニズムを分子レベルで解明するため、本研究では、申請者らが共同で開発した高解像度タンパク質複合体分離技術にピコ秒単位での分光学的測定、網羅的な複合体プロテオミクス、RNAseqを組み合わせ、トドマツ(マツ科)、イチイ(イチイ科)、ヒノキアスナロ(ヒノキ科)を対象に、冬期の常緑針葉樹に特有の光合成調節機構の総合的な理解を目指す。

Outline of Final Research Achievements

Generally, it is known that plants suffer damage due to photosynthetic electron transport at low temperatures. Evergreen trees prevent such damage by performing sustained thermal dissipation through photosystem II during wintertime. In this study, we conducted comprehensive analysis of RNA, proteins, protein complexes, photosynthetic pigments, and energy transfer related to photosynthesis with several different evergreen species including Taxus cuspidata, Abies sachalinensis, and a few other species, and indicated that a pigment-binding protein called early light-induced protein is involved in this thermal dissipation process.

Academic Significance and Societal Importance of the Research Achievements

植物が低温で光傷害を起こすという現象は広く知られている。このような光傷害への感受性には種間差がある。また、さまざまな環境要因が光傷害への感受性に影響をしている。本研究は、なぜ常緑樹は低温での光傷害に強いのか、という疑問を出発点として、その仕組の一端の解明につながる発見をした。とくにELIPという色素結合タンパク質が冬季の光化学系での熱放散に関わることを示すデータを得た。また、樹種によって、複数の熱放散の分子機構を持つ可能性を示す知見を得た。このような知見は、常緑樹の低温での光感受性の分子基盤として、将来、森林学、生態学の分野において活用されることが期待される。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (27 results)

All 2024 2023 2022 2021 Other

All Int'l Joint Research (4 results) Journal Article (9 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 9 results,  Open Access: 2 results) Presentation (14 results) (of which Int'l Joint Research: 2 results,  Invited: 5 results)

  • [Int'l Joint Research] Universidade Nova de Lisboa(ポルトガル)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Northern Illinois University(米国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Nanjing Univ Info Sci & Tech(中国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] University of Calcutta(インド)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Anthocyanins act as a sugar-buffer and an alternative electron sink in response to starch depletion during leaf senescence: a case study on a typical anthocyanic tree species, Acer japonicum2024

    • Author(s)
      Kitao M, Yazaki K, Tobita H, Agathokleous E, Kishimoto J, Takabayashi A, Tanaka R
    • Journal Title

      Journal of Experimental Botany

      Volume: erae109 Issue: 11 Pages: 3521-3541

    • DOI

      10.1093/jxb/erae109

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Dynamic seasonal changes in photosynthesis systems in leaves of Asarum tamaense, an evergreen understorey herbaceous species2023

    • Author(s)
      Naoki Wada, Issei Kondo, Ryouichi Tanaka, Junko Kishimoto, Atsuko Miyagi, Maki Kawai-Yamada, Yusuke Mizokami, Ko Noguchi
    • Journal Title

      Annals of Botany

      Volume: 131 Issue: 3 Pages: 423-436

    • DOI

      10.1093/aob/mcac156

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Characterization of the Chlorophyllide a Oxygenase (CAO) Enzyme Through an In Silico Approach2023

    • Author(s)
      Dey Debayan、Tanaka Ryouichi、Ito Hisashi
    • Journal Title

      Journal of Molecular Evolution

      Volume: 91 Issue: 2 Pages: 225-235

    • DOI

      10.1007/s00239-023-10100-9

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exposure to strong irradiance exacerbates photoinhibition and suppresses N resorption during leaf senescence in shade-grown seedlings of fullmoon maple (Acer japonicum)2022

    • Author(s)
      Kitao Mitsutoshi、Yazaki Kenichi、Tobita Hiroyuki、Agathokleous Evgenios、Kishimoto Junko、Takabayashi Atsushi、Tanaka Ryouichi
    • Journal Title

      Frontiers in Plant Science

      Volume: 13 Pages: 1006413-1006413

    • DOI

      10.3389/fpls.2022.1006413

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Heterologous complementation systems verify the mosaic distribution of three distinct protoporphyrinogen IX oxidase in the cyanobacterial phylum2022

    • Author(s)
      Kohata Ryoya、Lim HyunSeok、Kanamoto Yuki、Murakami Akio、Fujita Yuichi、Tanaka Ayumi、Swingley Wesley、Ito Hisashi、Tanaka Ryouichi
    • Journal Title

      Journal of Plant Research

      Volume: 136 Issue: 1 Pages: 107-115

    • DOI

      10.1007/s10265-022-01423-7

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Reversible down-regulation of photosystems I and II leads to fast photosynthesis recovery after long-term drought in Jatropha curcas2022

    • Author(s)
      Sapeta Helena、Yokono Makio、Takabayashi Atsushi、Ueno Yoshifumi、Cordeiro Andre M、Hara Toshihiko、Tanaka Ayumi、Akimoto Seiji、Oliveira M Margarida、Tanaka Ryouichi
    • Journal Title

      Journal of Experimental Botany

      Volume: 74 Issue: 1 Pages: 336-351

    • DOI

      10.1093/jxb/erac423

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Characterization of photosystem II assembly complexes containing ONE-HELIX PROTEIN1 in Arabidopsis thaliana2022

    • Author(s)
      Maeda Hanaki、Takahashi Koharu、Ueno Yoshifumi、Sakata Kei、Yokoyama Akari、Yarimizu Kozue、Myouga Fumiyoshi、Shinozaki Kazuo、Ozawa Shin-Ichiro、Takahashi Yuichiro、Tanaka Ayumi、Ito Hisashi、Akimoto Seiji、Takabayashi Atsushi、Tanaka Ryouichi
    • Journal Title

      Journal of Plant Research

      Volume: 135 Issue: 2 Pages: 361-376

    • DOI

      10.1007/s10265-022-01376-x

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insights into the structure and function of the rate-limiting enzyme of chlorophyll degradation through analysis of a bacterial Mg-dechelatase homolog2021

    • Author(s)
      Dey Debayan、Dhar Dipanjana、Fortunato Helena、Obata Daichi、Tanaka Ayumi、Tanaka Ryouichi、Basu Soumalee、Ito Hisashi
    • Journal Title

      Computational and Structural Biotechnology Journal

      Volume: 19 Pages: 5333-5347

    • DOI

      10.1016/j.csbj.2021.09.023

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions2021

    • Author(s)
      Fukura Koki、Tanaka Ayumi、Tanaka Ryouichi、Ito Hisashi
    • Journal Title

      Journal of Plant Physiology

      Volume: 266 Pages: 153535-153535

    • DOI

      10.1016/j.jplph.2021.153535

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] 越冬中の常緑樹の光合成応答2024

    • Author(s)
      田中亮一
    • Organizer
      第65回日本植物生理学会年会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 林冠落葉樹落葉期から積雪期にかけての気温や光環境の季節変化に対する林床ササの光合成系の応答2023

    • Author(s)
      島田康平、岸本純子、高林厚史、田中亮一、小野清美
    • Organizer
      日本植物学会第87回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 常緑樹は低温下でどのように光障害を防いでいるのか?2023

    • Author(s)
      田中亮一
    • Organizer
      第134回日本森林学会大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 寒冷圏の常緑樹はどのようにして低温下で光化学系を保護しているのだろうか?2022

    • Author(s)
      田中亮一
    • Organizer
      組織と材質研究会2022年冬季シンポジウム「樹木の越冬メカニズム」
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 寒冷圏の常緑樹はなぜ低温下でも緑を保つことができるのか?2022

    • Author(s)
      田中亮一
    • Organizer
      日本生化学会北海道支部会特別講演会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 常緑広葉樹ツルマサキの光化学系の年間変動2022

    • Author(s)
      下原かのこ、岸本純子、高林厚史、田中 亮一
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Revisiting the ELIP hypothesis for sustained thermal dissipation in overwintering yew leaves2022

    • Author(s)
      Zihao Ye, Mina Sawada, Makiko Iwasa, Ryo Moriyama, Mitsutoshi Kitao, Toshihiko Hara, Ayumi Tanaka, Junko Kishimoto, Makio Yokono, Atsushi Takabayashi, Ryouichi Tanaka
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 常緑針葉樹4種における冬季の光合成色素・光化学系タンパク質の変動2022

    • Author(s)
      成田あゆ、小野清美、高林厚史、菅井徹人、北尾光俊、田中 亮一
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Revisiting the ELIP hypothesis for sustained thermal dissipation in overwintering yew leaves2022

    • Author(s)
      Ryouichi Tanaka, Ye Zihao, Mina Sawada, Makio Yokono, Makiko Iwasa, Ryo Moriyama, Toshihiko Hara, Ayumi Tanaka, Mitsutoshi Kitao, Junko Kishimoto, Atsushi Takabayashi
    • Organizer
      International Congress on Photosynthesis Research 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Exploring the molecular mechanisms for sustained thermal dissipation in overwintering yew leaves2022

    • Author(s)
      Ryouichi Tanaka
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Regulation
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ツルマサキにおける α-carotene および lactucaxanthin の光化学系複合体への結合2022

    • Author(s)
      下原かのこ,高林厚史,田中亮一
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 光化学系タンパク質複合体の安定な分離のための CN-PAGE の改変2022

    • Author(s)
      亀尾辰砂,松前れのん,田中亮一,高林厚史
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Changes in the transcriptomes and the photosynthetic responses in overwintering Yew leaves2021

    • Author(s)
      Ye Zihao, Mina Sawada, Ryo Moriyama, Makiko Iwasa, Toshihiko Hara, Ayumi Tanaka, Atsushi Takabayashi, Ryouichi Tanaka: Changes in the transcriptomes and the photosynthetic responses in overwintering Yew leaves
    • Organizer
      第7回 北大・部局横断シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] Correlation of winter-specific gene expression and sustained thermal dissipation in over-wintering yew leaves2021

    • Author(s)
      Zihao Ye, Mina Sawada, Ryo Moriyama, Toshihiko Hara, Ayumi Tanaka, Atsushi, Takabayashi, Ryouichi Tanaka
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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