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Molecular mechanisms underlying heterochromatin assembly

Research Project

Project/Area Number 20H03189
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 43010:Molecular biology-related
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Nakayama Jun-ichi  基礎生物学研究所, クロマチン制御研究部門, 教授 (60373338)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2022: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2021: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2020: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywords遺伝子 / 発現制御 / ヘテロクロマチン / 分裂酵母 / ヒストン修飾
Outline of Research at the Start

ヘテロクロマチンは染色体の機能や遺伝子の発現調節に重要な役割を果たしている。ヒストンH3のメチル化修飾は、ヘテロクロマチンの重要なマークとして働き、進化的に保存されたSUV39H/Clr4メチル化酵素によって触媒される。分裂酵母のClr4はRik1やCul4などとともにCLRC複合体を形成しているが、その機能には不明な点が多い。本研究ではCLRCの機能解析を進め、ヘテロクロマチン構造形成の分子機構の解明を目指している。

Outline of Final Research Achievements

In this study, we focused on Clr4, a histone H3K9 methyltransferase that plays an important role in heterochromatin assembly in fission yeast, and the Clr4 methyltransferase complex (CLRC), and examined how their activities and functions are regulated. We found that Clr4 interacts directly with one of the CLRC components via the C-terminal SET domain-containing region. We also found that the methyltransferase activity of Clr4 is autoregulated by intramolecular interactions between its N- and C-terminal regions.

Academic Significance and Societal Importance of the Research Achievements

真核細胞の染色体に存在するヘテロクロマチンは、染色体の機能やゲノム恒常性の維持、エピジェネティックな遺伝子発現制御に重要な役割を果たしている。ヒストンのメチル化修飾はヘテロクロマチンの重要なマークであり、そのメチル化修飾の制御機構を明らかにする本研究は、高次生命現象を理解する上で重要な課題である。本研究は、ヒストンのメチル化酵素Clr4の制御機構の一端を明らかにした画期的な成果であり、進化的に保存されたヒストンメチル化酵素の活性制御の分子機構の理解にもつながる成果である。

Report

(4 results)
  • 2023 Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (17 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (2 results) Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (11 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Int'l Joint Research] University of Toronto(カナダ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Tianjin Medical University(中国)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Regulation of the SUV39H Family Methyltransferases: Insights from Fission Yeast2023

    • Author(s)
      Nakamura Rinko、Nakayama Jun-ichi
    • Journal Title

      Biomolecules

      Volume: 13 Issue: 4 Pages: 593-593

    • DOI

      10.3390/biom13040593

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multiple interfaces to recognize nucleosomal targets2021

    • Author(s)
      Rinko Nakamura, Jun-ichi Nakayama
    • Journal Title

      The Journal of Biochemistry

      Volume: 171 Issue: 3 Pages: 257-259

    • DOI

      10.1093/jb/mvab139

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomics-Based Systematic Identification of Nuclear Proteins Anchored to Chromatin via RNA2020

    • Author(s)
      Kyoko Hiragami-Hamada, Naoki Tani, Jun-Ichi Nakayama
    • Journal Title

      Methods in Molecular Biology

      Volume: 2161 Pages: 89-99

    • DOI

      10.1007/978-1-0716-0680-3_8

    • ISBN
      9781071606797, 9781071606803
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for histone variant H3tK27me3 recognition by PHF1 and PHF192020

    • Author(s)
      Cheng Dong, Reiko Nakagawa, Kyohei Oyama, Yusuke Yamamoto, Weilian Zhang, Aiping Dong, Yanjun Li, Yuriko Yoshimura, Hiroyuki Kamiya, Jun-Ichi Nakayama, Jun Ueda, Jinrong Min
    • Journal Title

      Elife

      Volume: 9

    • DOI

      10.7554/elife.58675

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Mechanisms regulating Clr4/SUV39H histone methyltransferase activity2022

    • Author(s)
      Rinko Nakamura, Yuriko Yoshimura, Reiko Nakagawa, Jun-ichi Nakayama
    • Organizer
      Japan-UK Regulation through Chromatin Conference
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Histone modifications and higher-order chromatin assembly2022

    • Author(s)
      Jun-ichi Nakayama
    • Organizer
      NIBB-COS Workshop in Heidelberg
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ヒストンメチル化酵素Clr4の活性制御機構の解析2022

    • Author(s)
      中村凜子、吉村ゆり子、中川れい子、中山潤一
    • Organizer
      第95回日本生化学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ヒストンメチル化酵素Clr4の活性制御機構の解析2022

    • Author(s)
      中村凜子、吉村ゆり子、中川れい子、中山潤一
    • Organizer
      第45回日本分子生物学会年会 (MBSJ2022)
    • Related Report
      2022 Annual Research Report
  • [Presentation] Mechanisms regulating Clr4 histone methyltransferase activity2022

    • Author(s)
      Rinko Nakamura, Yuriko Yoshimura, Reiko Nakagawa, Jun-ichi Nakayama
    • Organizer
      The 30th Hot Spring Harbor International Symposium, Chromatin Potential in Development and Differentiation & The 6th Symposium of the Inter-University Research Network for Trans-Omics Medicine
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 分裂酵母のヒストンメチル化酵素Clr4の活性制御機構の解析2021

    • Author(s)
      中村凜子、吉村 ゆり子、中山 潤一
    • Organizer
      酵母遺伝学フォーラム第54回研究報告会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ヒストンメチル化酵素Clr4の活性制御機構の解析2021

    • Author(s)
      中村凜子、吉村ゆり子、中川れい子、中山潤一
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ヒストンメチル化酵素 Clr4 の活性制御機構の解析2021

    • Author(s)
      中村 凜子、吉村 ゆり子、中川 れい子、中山 潤一
    • Organizer
      第39回染色体ワークショップ・第20回核ダイナミクス研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 分裂酵母ヒストンメチル化酵素複合体CLRCの機能解析2021

    • Author(s)
      蜂須賀亜紀、沖昌也、中山潤一
    • Organizer
      第14回 日本エピジェネティクス研究会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 分裂酵母ヒストンメチル化酵素複合体CLRCの機能解析2020

    • Author(s)
      蜂須賀亜紀、沖昌也、中山潤一
    • Organizer
      第53回 酵母遺伝学フォーラム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 分裂酵母ヒストンメチル化酵素複合体CLRCの機能解析2020

    • Author(s)
      蜂須賀亜紀、沖昌也、中山潤一
    • Organizer
      日本分子生物学会 第43回年会
    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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