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Structural analysis of the oxygen evolving center of photosystem II to gain insights into the water splitting reaction

Research Project

Project/Area Number 20H03226
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 43040:Biophysics-related
Research InstitutionOkayama University

Principal Investigator

Suga Michihiro  岡山大学, 異分野基礎科学研究所, 教授 (60634920)

Co-Investigator(Kenkyū-buntansha) 中島 芳樹  岡山大学, 異分野基礎科学研究所, 特任助教 (60847052)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2022: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2021: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords光合成 / 膜タンパク質 / 光化学系II / 水分解反応 / X線自由電子レーザー
Outline of Research at the Start

光合成では光化学系IIとよばれるタンパク質が光のエネルギーを利用して水分子から電子と水素イオンを取り出して酸素分子を発生する。この反応は光化学系IIの “ゆがんだイス”型のマンガンクラスター触媒が5つの周期的な段階をとることで進む。本研究ではS2状態とよばれる段階を構造解析し,光化学系IIの“ゆがんだイス”型のマンガンクラスター触媒が次の段階に遷移するときの構造変化から反応機構の詳細を明らかにする。

Outline of Final Research Achievements

To determine the functional differences in D1 proteins expressed from psbA genes, PSII dimers from two strains expressing only one psbA gene (psbA2 or psbA3, respectively) were crystallized and structurally analyzed at a resolution comparable to that of PsbA1-PSII, which has been studied previously. We found that in PsbA2- and PsbA3-PSII, the change from Gln to Glu strengthens the hydrogen bond between pheophytin/D1 (PheoD1) and D1-130, partially explaining the increased redox potential of PheoD1 observed in PsbA3. These results were published in JBC.

Data for the high-spin S2 state are also currently being analyzed.

Academic Significance and Societal Importance of the Research Achievements

光化学系IIはタンパク質複合体であり、触媒部分のMn4CaO5 のクラスター(酸素発生中心)はD1タンパク質に保持されている。D1タンパク質はPsbA遺伝子によってコードされているが、通常の条件ではPsbA1遺伝子が発現する。一方、乾燥条件などの特殊な条件ではPsbA2遺伝子またはPsbA3遺伝子が発現する。PsbA2およびPsbA3を発現した光化学系IIを結晶構造解析することで、これらPsbAタンパク質の役割を明らかにすることを目指したものである。本研究で得られた立体構造は、3つのPsbAバリアントの機能的な検証のための構造的な基礎を提供するものと考えられる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (13 results)

All 2022 2021

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (9 results) (of which Int'l Joint Research: 5 results,  Invited: 5 results) Book (1 results)

  • [Journal Article] Crystal structures of photosystem II from a cyanobacterium expressing psbA in comparison to psbA reveal differences in the D1 subunit2022

    • Author(s)
      Nakajima Yoshiki、Ugai-Amo Natsumi、Tone Naoki、Nakagawa Akiko、Iwai Masako、Ikeuchi Masahiko、Sugiura Miwa、Suga Michihiro、Shen Jian-Ren
    • Journal Title

      Journal of Biological Chemistry

      Volume: 298 Issue: 12 Pages: 102668-102668

    • DOI

      10.1016/j.jbc.2022.102668

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Geometric, electronic and spin structures of the CaMn4O5 catalyst for water oxidation in oxygen-evolving photosystem II. Interplay between experiments and theoretical computations2022

    • Author(s)
      Yamaguchi Kizashi、Shoji Mitsuo、Isobe Hiroshi、Kawakami Takashi、Miyagawa Koichi、Suga Michihiro、Akita Fusamichi、Shen Jian-Ren
    • Journal Title

      Coordination Chemistry Reviews

      Volume: 471 Pages: 214742-214742

    • DOI

      10.1016/j.ccr.2022.214742

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Capturing structural changes of the S1 to S2 transition of photosystem II using time-resolved serial femtosecond crystallography2021

    • Author(s)
      H Li, Y Nakajima, T Nomura, M Sugahara, S Yonekura, SK Chan, T Nakane, T Yamane, Y Umena, M Suzuki, T Masuda, T Motomura, H Naitow, Y Matsuura, T Kimura, K Tono, S Owada, Y Joti, R Tanaka, E Nango, F Akita, M Kubo, S Iwata, JR Shen and M Suga
    • Journal Title

      IUCrJ

      Volume: 8 Issue: 3 Pages: 431-443

    • DOI

      10.1107/s2052252521002177

    • NAID

      120007037379

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Structural dynamics and substrate binding to the oxygen-evolving complex studied by time-resolved X-ray crystallography of PSII2022

    • Author(s)
      M Suga
    • Organizer
      10th Asian Biological Inorganic Chemnistry Conference
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] X線自由電子レーザーを用いた解析におる光化学系IIの基質水分子の取り込みと水分子の酸化に関する構造的知見2022

    • Author(s)
      菅 倫寛, 中島芳樹, リホンジェ, 沈 建仁
    • Organizer
      第60回 生物物理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Studies on the molecular mechanism for water oxidation in photosystem II2022

    • Author(s)
      菅 倫寛
    • Organizer
      The 22nd annual meeting of the photobiology association of Japan
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Time-resolved structural studies on photosystem II provide insights into the mechanism of water oxidation and substrate delivery2022

    • Author(s)
      M Suga
    • Organizer
      Satellite Meeting to 18th international congress on photosynthesis research 2022: Mechanism of water oxidation
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] X-ray free electron lasers reveal the molecular mechanism for water oxidation in photosystem II.2022

    • Author(s)
      M Suga
    • Organizer
      International congress on photosynthesis research 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Crystal structure analyses of photosystem II isolated from Thermosynechococcus elongatus mutants expressing only psbA2 or psbA3 genes as the D1 protein.2022

    • Author(s)
      Nakajima Y., Ugai-Amo N., Tone N., Nakagawa A., Iwai M., Sugiura M., Ikeuchi M., Suga M. and Shen J. -R.
    • Organizer
      18th International Congress on Photosynthesis Research, Dunedin, New Zealand.
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光化学系IIのS状態遷移及び結晶品質における界面活性剤の影響2022

    • Author(s)
      中島芳樹、菅倫寛、沈建仁
    • Organizer
      第63回植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Capturing structural changes of photosystem II using time-resolved serial femtosecond crystallography2021

    • Author(s)
      Hongjie Li, Yoshiki Nakajima, Jian-Ren Shen and Michihiro Suga
    • Organizer
      PDB50 Anniversary Symposium in Asia
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] X-ray free electron lasers reveal the molecular mechanism for water oxidation in photosystem II2021

    • Author(s)
      Michi Suga, Yoshiki Nakajima, Hongjie Li, Jian-Ren Shen
    • Organizer
      第59回日本生物物理学会年会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Book] Photosynthesis: Molecular Approaches to Solar Energy Conversion2021

    • Author(s)
      Jian-Ren Shen, Yoshiki Nakajima, Fusamichi Akita & Michihiro Suga
    • Total Pages
      622
    • Publisher
      Springer Cham
    • ISBN
      9783030674069
    • Related Report
      2021 Annual Research Report

URL: 

Published: 2020-04-28   Modified: 2024-01-30  

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