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Study of the mechanisms of PIN cluster formation, a crucial phenomenon in polar auxin transport

Research Project

Project/Area Number 20H03286
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44040:Morphology and anatomical structure-related
Research InstitutionHokkaido University

Principal Investigator

Naramoto Satoshi  北海道大学, 理学研究院, 准教授 (30612022)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2022: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2021: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Keywords細胞極性 / 体軸 / オーキシン / PIN / クラスター / 相分離 / MAB4 / シロイヌナズナ / cluster / PINクラスター / 進化 / 蘚苔類 / 極性 / ゼニゴケ
Outline of Research at the Start

植物では、植物ホルモンであるオーキシンが組織中を極性輸送(一定の方向に輸送されること)されることで、体軸が形成されるが、その方向はオーキシン排出担体PINの細胞における偏在化により構築される。
従来の研究では、PINの局在制御は小胞輸送系に大きく依存すると考えられてきたが、申請者は、PINの偏在化には、小胞輸送系だけでは十分でなく、「PINの細胞膜中の流動性を制限する機構」が別に必要となることを見出した。本研究では、申請者が発見した本メカニズムの分子レベルでの理解を進めるとともに、本機構の普遍性・多様性について解析を行う。

Outline of Final Research Achievements

Body axis formation is a phenomenon that underlies morphogenesis in living organisms. The body axis of a plant is defined by the polar localization of the auxin efflux carrier PIN in the cell, and a model in which PIN is continuously transported in vesicles to a specific site has been proposed. On the other hand, I found that PIN forms multimeric clusters which do not undergo diffusion or endocytosis. I have also identified a MAB4 protein that is responsible for the formation of PIN clusters. In this study, I performed a detailed analysis of PIN and MAB4 proteins. I discovered that PIN and MAB4 can interact with each other. I also found that PIN clustering plays an important role in the polar localization of PIN. Finally, I also found that PIN cluster formation is conserved phenomenon among land plants.

Academic Significance and Societal Importance of the Research Achievements

体軸形成は、生物の形態形成を支える現象のひとつである。植物では、オーキシンの極性輸送により体軸が形成されるが、その輸送方向は、オーキシン排出担体PINの細胞における偏在によって規定されている。そのため、本研究によりPINの局在化機構を解明することは、植物の形態形成を理解する上で重要である。とりわけ、植物の多様な形態がどのように進化してきたかを明らかにすることにも繋がることから、学術上極めて重要である。また、本研究により得られる知見は、オーキシンの流路制御などの方策をもたらしうることから、植物形態のデザイン化などの農業利用の方策の礎となると考えられる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (17 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (10 results) Journal Article (7 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 7 results,  Open Access: 4 results)

  • [Int'l Joint Research] Umea plant science center(スウェーデン)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] University of Lausanne(スイス)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] University of Bristol(英国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] IST Austria(オーストリア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Leiden University(オランダ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] VIB Ghent University(ベルギー)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Charles University(チェコ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] BOKU(オーストリア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] IST Austria(オーストリア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Leiden university(オランダ)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Control of vegetative reproduction in Marchantia polymorpha by the KAI2-ligand signaling pathway2023

    • Author(s)
      Komatsu Aino、Kodama Kyoichi、Mizuno Yohei、Fujibayashi Mizuki、Naramoto Satoshi、Kyozuka Junko
    • Journal Title

      Current Biology

      Volume: 33 Issue: 7 Pages: 1196-1210

    • DOI

      10.1016/j.cub.2023.02.022

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The bryophytes Physcomitrium patens and Marchantia polymorpha as model systems for studying evolutionary cell and developmental biology in plants.2022

    • Author(s)
      Naramoto, S., Hata, Y., Fujita T, and Kyozuka J.
    • Journal Title

      The Plant Cell

      Volume: 34 Issue: 1 Pages: 228-246

    • DOI

      10.1093/plcell/koab218

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells2021

    • Author(s)
      Glanc M, Van Gelderen K, Hoermayer L, Tan S, Naramoto S, Zhang X, Domjan D, Vcelarova L, Hauschild R, Johnson A, de Koning E, van Dop M, Rademacher E, Janson S, Wei X, Molnar G, Fendrych M, De Rybel B, Offringa R, Friml J.
    • Journal Title

      Curr Biol

      Volume: - Issue: 9 Pages: 1918-1930.e5

    • DOI

      10.1016/j.cub.2021.02.028

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] NARROW AND DWARF LEAF 1, the Ortholog of Arabidopsis ENHANCER OF SHOOT REGENERATION1/DORNROSCHEN, Mediates Leaf Development and Maintenance of the Shoot Apical Meristem in Oryza sativa L2021

    • Author(s)
      Kusnandar Andree S、Itoh Jun-Ichi、Sato Yutaka、Honda Eriko、Hibara Ken-ichiro、Kyozuka Junko、Naramoto Satoshi
    • Journal Title

      Plant and Cell Physiology

      Volume: 63 Issue: 2 Pages: 265-278

    • DOI

      10.1093/pcp/pcab169

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Major components of the KARRIKIN INSENSITIVE2-dependent signaling pathway are conserved in the liverwort Marchantia polymorpha2021

    • Author(s)
      Mizuno Y, Komatsu A, Shimazaki S, Naramoto S, Inoue K, Xie X, Ishizaki K, Kohchi T, Kyozuka J.
    • Journal Title

      Plant Cell

      Volume: - Issue: 7 Pages: 112-156

    • DOI

      10.1093/plcell/koab106

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 1062020

    • Author(s)
      Wachananawat Bussarin、Kuroha Takeshi、Takenaka Yuto、Kajiura Hiroyuki、Naramoto Satoshi、Yokoyama Ryusuke、Ishizaki Kimitsune、Nishitani Kazuhiko、Ishimizu Takeshi
    • Journal Title

      Frontiers in Plant Science

      Volume: 11 Pages: 997-997

    • DOI

      10.3389/fpls.2020.00997

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana2020

    • Author(s)
      Li Hongjiang、Wangenheim Daniel、Zhang Xixi、Tan Shutang、Darwish‐Miranda Nasser、Naramoto Satoshi、Wabnik Krzysztof、De Rycke Riet、Kaufmann Walter A.、Gutl Daniel、Tejos Ricardo、Grones Peter、Ke Meiyu、Chen Xu、Dettmer Jan、Friml Jiri
    • Journal Title

      New Phytologist

      Volume: 229 Issue: 1 Pages: 351-369

    • DOI

      10.1111/nph.16887

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research

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Published: 2020-04-28   Modified: 2024-01-30  

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