Study of the mechanisms of PIN cluster formation, a crucial phenomenon in polar auxin transport

• Project/Area Number: 20H03286
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 44040:Morphology and anatomical structure-related
• Research Institution: Hokkaido University
• Principal Investigator: Naramoto Satoshi 北海道大学, 理学研究院, 准教授 (30612022)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000); Fiscal Year 2022: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2021: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2020: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
• Keywords: 細胞極性 / 体軸 / オーキシン / PIN / クラスター / 相分離 / MAB4 / シロイヌナズナ / cluster / PINクラスター / 進化 / 蘚苔類 / 極性 / ゼニゴケ

Outline of Research at the Start

植物では、植物ホルモンであるオーキシンが組織中を極性輸送(一定の方向に輸送されること)されることで、体軸が形成されるが、その方向はオーキシン排出担体PINの細胞における偏在化により構築される。
従来の研究では、PINの局在制御は小胞輸送系に大きく依存すると考えられてきたが、申請者は、PINの偏在化には、小胞輸送系だけでは十分でなく、「PINの細胞膜中の流動性を制限する機構」が別に必要となることを見出した。本研究では、申請者が発見した本メカニズムの分子レベルでの理解を進めるとともに、本機構の普遍性・多様性について解析を行う。

Outline of Final Research Achievements

Body axis formation is a phenomenon that underlies morphogenesis in living organisms. The body axis of a plant is defined by the polar localization of the auxin efflux carrier PIN in the cell, and a model in which PIN is continuously transported in vesicles to a specific site has been proposed. On the other hand, I found that PIN forms multimeric clusters which do not undergo diffusion or endocytosis. I have also identified a MAB4 protein that is responsible for the formation of PIN clusters. In this study, I performed a detailed analysis of PIN and MAB4 proteins. I discovered that PIN and MAB4 can interact with each other. I also found that PIN clustering plays an important role in the polar localization of PIN. Finally, I also found that PIN cluster formation is conserved phenomenon among land plants.

Academic Significance and Societal Importance of the Research Achievements

体軸形成は、生物の形態形成を支える現象のひとつである。植物では、オーキシンの極性輸送により体軸が形成されるが、その輸送方向は、オーキシン排出担体PINの細胞における偏在によって規定されている。そのため、本研究によりPINの局在化機構を解明することは、植物の形態形成を理解する上で重要である。とりわけ、植物の多様な形態がどのように進化してきたかを明らかにすることにも繋がることから、学術上極めて重要である。また、本研究により得られる知見は、オーキシンの流路制御などの方策をもたらしうることから、植物形態のデザイン化などの農業利用の方策の礎となると考えられる。

Research Products

• [Int'l Joint Research] Umea plant science center(スウェーデン)
• [Int'l Joint Research] University of Lausanne(スイス)
• [Int'l Joint Research] University of Bristol(英国)
• [Int'l Joint Research] IST Austria(オーストリア)
• [Int'l Joint Research] Leiden University(オランダ)
• [Int'l Joint Research] VIB Ghent University(ベルギー)
• [Int'l Joint Research] Charles University(チェコ)
• [Int'l Joint Research] BOKU(オーストリア)
• [Int'l Joint Research] IST Austria(オーストリア)
• [Int'l Joint Research] Leiden university(オランダ)
• [Journal Article] Control of vegetative reproduction in Marchantia polymorpha by the KAI2-ligand signaling pathway (2023)
  • Author(s): Komatsu Aino、Kodama Kyoichi、Mizuno Yohei、Fujibayashi Mizuki、Naramoto Satoshi、Kyozuka Junko
  • Journal Title: Current Biology Volume: 33 Issue: 7 Pages: 1196-1210.e4
  • DOI: 10.1016/j.cub.2023.02.022
  • Peer Reviewed
• [Journal Article] The bryophytes Physcomitrium patens and Marchantia polymorpha as model systems for studying evolutionary cell and developmental biology in plants. (2022)
  • Author(s): Naramoto, S., Hata, Y., Fujita T, and Kyozuka J.
  • Journal Title: The Plant Cell Volume: 34 Issue: 1 Pages: 228-246
  • DOI: 10.1093/plcell/koab218
  • Peer Reviewed
• [Journal Article] AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells (2021)
  • Author(s): Glanc M, Van Gelderen K, Hoermayer L, Tan S, Naramoto S, Zhang X, Domjan D, Vcelarova L, Hauschild R, Johnson A, de Koning E, van Dop M, Rademacher E, Janson S, Wei X, Molnar G, Fendrych M, De Rybel B, Offringa R, Friml J.
  • Journal Title: Curr Biol Volume: - Issue: 9 Pages: 1918-1930.e5
  • DOI: 10.1016/j.cub.2021.02.028
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] NARROW AND DWARF LEAF 1, the Ortholog of Arabidopsis ENHANCER OF SHOOT REGENERATION1/DORNROSCHEN, Mediates Leaf Development and Maintenance of the Shoot Apical Meristem in Oryza sativa L (2021)
  • Author(s): Kusnandar Andree S、Itoh Jun-Ichi、Sato Yutaka、Honda Eriko、Hibara Ken-ichiro、Kyozuka Junko、Naramoto Satoshi
  • Journal Title: Plant and Cell Physiology Volume: 63 Issue: 2 Pages: 265-278
  • DOI: 10.1093/pcp/pcab169
  • Peer Reviewed
• [Journal Article] Major components of the KARRIKIN INSENSITIVE2-dependent signaling pathway are conserved in the liverwort Marchantia polymorpha (2021)
  • Author(s): Mizuno Y, Komatsu A, Shimazaki S, Naramoto S, Inoue K, Xie X, Ishizaki K, Kohchi T, Kyozuka J.
  • Journal Title: Plant Cell Volume: - Issue: 7 Pages: 112-156
  • DOI: 10.1093/plcell/koab106
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106 (2020)
  • Author(s): Wachananawat Bussarin、Kuroha Takeshi、Takenaka Yuto、Kajiura Hiroyuki、Naramoto Satoshi、Yokoyama Ryusuke、Ishizaki Kimitsune、Nishitani Kazuhiko、Ishimizu Takeshi
  • Journal Title: Frontiers in Plant Science Volume: 11 Pages: 997-997
  • DOI: 10.3389/fpls.2020.00997
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana (2020)
  • Author(s): Li Hongjiang、Wangenheim Daniel、Zhang Xixi、Tan Shutang、Darwish‐Miranda Nasser、Naramoto Satoshi、Wabnik Krzysztof、De Rycke Riet、Kaufmann Walter A.、Gutl Daniel、Tejos Ricardo、Grones Peter、Ke Meiyu、Chen Xu、Dettmer Jan、Friml Jiri
  • Journal Title: New Phytologist Volume: 229 Issue: 1 Pages: 351-369
  • DOI: 10.1111/nph.16887
  • Peer Reviewed / Open Access / Int'l Joint Research