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Mechanisms of cortical folding focusing on axon guidance

Research Project

Project/Area Number 20H03351
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 46020:Anatomy and histopathology of nervous system-related
Research InstitutionHamamatsu University School of Medicine (2022-2023)
Kanazawa University (2020-2021)

Principal Investigator

Shinmyo Yohei  浜松医科大学, 医学部, 教授 (00418831)

Co-Investigator(Kenkyū-buntansha) 河崎 洋志  金沢大学, 医学系, 教授 (50303904)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2022: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2021: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywordsフェレット / 脳回脳溝形成 / 脳回 / 大脳皮質 / 軸索ガイダンス
Outline of Research at the Start

進化におけるシワ(脳回)の獲得は高次脳機能の発達の基盤であり、従ってその形成メカニズムの解明は神経科学の重要研究課題の一つである。我々は脳回など高等哺乳動物に特徴的な発達した脳神経構築を持つフェレットをモデル動物として採用している。本研究では、軸索投射という独自の視点から脳回の形成機構解明を目指す。

Outline of Final Research Achievements

Folding and expansion of the cerebral cortex during mammalian evolution are considered to be crucial advances in acquiring higher brain functions. We have elucidated the mechanism of cortical folding using ferrets with well-developed brain structures. The next important task is to understand the mechanism of pattern formation of cortical folds. In this study, we examined the importance of neural progenitor cells, neurons, axon projections and glial cells in the pattern formation of cortical folds. We found that astrocytes are more abundant in the gyrus than in the sulcus of the ferret brain. Furthermore, we demonstrated that this local production of astrocytes in the gyrus is important for cortical folding.

Academic Significance and Societal Importance of the Research Achievements

皺脳動物でのアストロサイト研究の重要性は世界的に注目されていたが、その研究は遅れていた。この主たる原因は、皺脳動物の脳内のアストロサイトでの遺伝子操作が困難なためであった。重要なことに私たちは、子宮内エレクトロポレーション法、piggyBacシステム、細胞種特異的プロモーターを組みわせることにより、フェレット大脳においてアストロサイト選択的な遺伝子発現システムを確立した。その手法を用いて、脳回形成におけるアストロサイトの重要性を明らかにした。このように、独自技術を利用して皺脳動物でのアストロサイトの解析を行った本課題は学術的に重要性の高い研究であると言える。

Report

(4 results)
  • 2023 Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (14 results)

All 2024 2023 2022 2021

All Journal Article (10 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 10 results,  Open Access: 9 results) Presentation (4 results)

  • [Journal Article] Distinct subdivisions of subcortical U-fiber regions in the gyrencephalic ferret brain2024

    • Author(s)
      Yoshino Mayuko、Shiraishi Yoshitake、Saito Kengo、Kameya Narufumi、Hamabe-Horiike Toshihide、Shinmyo Yohei、Nakada Mitsutoshi、Ozaki Noriyuki、Kawasaki Hiroshi
    • Journal Title

      Neuroscience Research

      Volume: 200 Pages: 1-7

    • DOI

      10.1016/j.neures.2023.10.004

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Early establishment of chloride homeostasis in CRH neurons is altered by prenatal stress leading to fetal HPA axis dysregulation2024

    • Author(s)
      Watanabe Miho、Sinha Adya Saran、Shinmyo Yohei、Fukuda Atsuo
    • Journal Title

      Frontiers in Molecular Neuroscience

      Volume: 17 Pages: 1373337-1373337

    • DOI

      10.3389/fnmol.2024.1373337

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Shank3a/b isoforms regulate the susceptibility to seizures and thalamocortical development in the early postnatal period of mice2023

    • Author(s)
      Okuzono Sayaka、Fujii Fumihiko、Matsushita Yuki、Setoyama Daiki、Shinmyo Yohei、Taira Ryoji、Yonemoto Kousuke、Akamine Satoshi、Motomura Yoshitomo、Sanefuji Masafumi、Sakurai Takeshi、Kawasaki Hiroshi、Han Kihoon、Kato Takahiro A.、Torisu Hiroyuki、Kang Dongchon、Nakabeppu Yusaku、Sakai Yasunari、Ohga Shouichi
    • Journal Title

      Neuroscience Research

      Volume: in press Pages: 13-19

    • DOI

      10.1016/j.neures.2023.03.001

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A New Technical Approach for Cross-species Examination of Neuronal Wiring and Adult Neuron-glia Functions2023

    • Author(s)
      Edwards-Faret Gabriela、de Vin Filip、Slezak Michal、Gollenbeck Lennart、Karaman Ruchan、Shinmyo Yohei、Batiuk Mykhailo Y.、Pando Carmen Menacho、Urschitz Johann、Rincon Melvin Y.、Moisyadi Stefan、Schnutgen Frank、Kawasaki Hiroshi、Schmucker Dietmar、Holt Matthew G.
    • Journal Title

      Neuroscience

      Volume: 508 Pages: 40-51

    • DOI

      10.1016/j.neuroscience.2022.11.029

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Isolation of ferret astrocytes reveals their morphological, transcriptional, and functional differences from mouse astrocytes2022

    • Author(s)
      Roboon Jureepon、Hattori Tsuyoshi、Nguyen Dinh Thi、Ishii Hiroshi、Takarada-Iemata Mika、Kannon Takayuki、Hosomichi Kazuyoshi、Maejima Takashi、Saito Kengo、Shinmyo Yohei、Mieda Michihiro、Tajima Atsushi、Kawasaki Hiroshi、Hori Osamu
    • Journal Title

      Frontiers in Cellular Neuroscience

      Volume: 16 Pages: 1-15

    • DOI

      10.3389/fncel.2022.877131

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Investigation of the Mechanisms Underlying the Development and Evolution of the Cerebral Cortex Using Gyrencephalic Ferrets2022

    • Author(s)
      Shinmyo Yohei、Hamabe-Horiike Toshihide、Saito Kengo、Kawasaki Hiroshi
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 10 Pages: 847159-847159

    • DOI

      10.3389/fcell.2022.847159

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Localized astrogenesis regulates gyrification of the cerebral cortex2022

    • Author(s)
      Shinmyo Yohei、Saito Kengo、Hamabe-Horiike Toshihide、Kameya Narufumi、Ando Akitaka、Kawasaki Kanji、Duong Tung Anh Dinh、Sakashita Masataka、Roboon Jureepon、Hattori Tsuyoshi、Kannon Takayuki、Hosomichi Kazuyoshi、Slezak Michal、Holt Matthew G.、Tajima Atsushi、Hori Osamu、Kawasaki Hiroshi
    • Journal Title

      Science Advances

      Volume: 8 Issue: 10 Pages: 1-15

    • DOI

      10.1126/sciadv.abi5209

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation2021

    • Author(s)
      Hamabe-Horiike Toshihide、Kawasaki Kanji、Sakashita Masataka、Ishizu Chihiro、Yoshizaki Tomokazu、Harada Shin-ichi、Ogawa-Ochiai Keiko、Shinmyo Yohei、Kawasaki Hiroshi
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 4864-4864

    • DOI

      10.1038/s41598-021-84210-z

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The expression of aristaless-related homeobox in neural progenitors of gyrencephalic carnivore ferrets2021

    • Author(s)
      Maeyama Hiroki、Shinmyo Yohei、Kawasaki Hiroshi
    • Journal Title

      Biochemistry and Biophysics Reports

      Volume: 26 Pages: 100970-100970

    • DOI

      10.1016/j.bbrep.2021.100970

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dysfunction of the proteoglycan Tsukushi causes hydrocephalus through altered neurogenesis in the subventricular zone in mice2021

    • Author(s)
      Ito Naofumi、(24/28)
    • Journal Title

      Science Translational Medicine

      Volume: 13 Issue: 587 Pages: 7896-7896

    • DOI

      10.1126/scitranslmed.aay7896

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Localized astrogenesis controls gyrification of the cerebral cortex2023

    • Author(s)
      Shinmyo Y and Kawasaki H
    • Organizer
      MBSJ2023
    • Related Report
      2022 Annual Research Report
  • [Presentation] A positive feedback loop of FGF signaling induces astrocyte expansion and folding of the cerebral cortex in gyrencephalic animals2023

    • Author(s)
      Shinmyo Y and Kawasaki H
    • Organizer
      日本解剖学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] A positive feedback loop of FGF signaling induces astrocyte expansion and folding of the cerebral cortex in gyrencephalic animals2022

    • Author(s)
      Shinmyo Y and Kawasaki H
    • Organizer
      Development and Plasticity of the Brain
    • Related Report
      2021 Annual Research Report
  • [Presentation] A positive feedback loop of FGF signaling induces astrocyte expansion and folding of the cerebral cortex in gyrencephalic animals2022

    • Author(s)
      Shinmyo Y, Saito K, Hamabe-Horiike T, Kameya N, Hattori T, Kannon T, Hosomichi K, Tajima A, Hori O and Kawasaki H
    • Organizer
      NEURO2022
    • Related Report
      2021 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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