| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2023: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2022: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2021: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2020: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Outline of Final Research Achievements |
A novel mechanism for somatic stem cell differentiation via phagocytosing apoptotic differentiated cells was found to require only a short time frame. Pluripotent Muse cells phagocytosed apoptotic differentiated cells. The phagocytosed-differentiated cell-derived contents (e.g., transcription factors) were quickly translocated into the nucleus, and led to differentiation initiation. The gene expression profiles were similar to those of the authentic differentiated cells and expressed functional markers in single cell RNA sequencing. iPS/ES cells did not show phagocytosis-induced differentiation activity. Together, our findings uncovered a simple mechanism by which differentiation-directing factors are directly transferred to somatic stem cells by phagocytosing apoptotic differentiated cells to trigger their rapid differentiation into the target cell lineage.
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