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Evaluation of Ecological Effects of Tritium Using Carcinogenesis Highly Susceptible Mouse Models

Research Project

Project/Area Number 20K05386
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 31010:Nuclear engineering-related
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

Umata Toshiyuki  産業医科大学, 教育研究支援施設, 准教授 (30213482)

Co-Investigator(Kenkyū-buntansha) 大津山 彰  産業医科大学, 医学部, 准教授 (10194218)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsトリチウム / 低線量率被ばく / 発がん高感受性モデルマウス / LOH解析 / 低線量率ひばく / 遺伝子変異
Outline of Research at the Start

トリチウムによる被ばくの形態は、放出されるベータ線のエネルギーが低いことや分子の状態(水蒸気)などから、低線量・低線量率の内部被ばくが想定される。我々は、低線量でのトリチウムベータ線の影響を調べるために、発がん高感受性マウス( ApcMin/+マウス:大腸癌抑制遺伝子であるAPCに変異があり、小腸・大腸癌が高頻度で発生する)を用いて突然変異の発生場所を調べ、トリチウムによる発がんの機構を解明する。

Outline of Final Research Achievements

The effect of low dose rate on carcinogenesis was examined by administering tritiated water to a carcinogenesis model mouse (ApcMin/+ mouse). 90 MBq of tritiated water was administered intraperitoneally, and the small intestine was estimated to be exposed to 2.4 Gy over 7 days. The results of tumor development showed that the number of tumors was significantly increased at 2.4 Gy compared to mice in the non-tritium water group, but no increase was observed at 1.2 Gy. Genomic DNA was extracted from tumor cells, and LOH analysis was performed using Mit marker on chromosome 18 to analyze the pattern of tritium-induced loss of function of cancer suppressor genes. No characteristic deletions were detected in mice in the tritiated water group compared to mice in the non-tritiated group.

Academic Significance and Societal Importance of the Research Achievements

低レベルのトリチウム暴露によって人体影響が出るかを、科学的なデータに基づいて議論するには、高感度の新規実験系を立ち上げて数値データを得ると共に、放射線障害の発生メカニズムや放射線に対する生体応答機構を遺伝子レベルで明らかにすることが必要である。遺伝子の突然変異や発がんなど、いわゆる確率的影響に関する研究は、今後ますます重要性が増してくるものと思われる。
トリチウムの生体影響研究者は世界的に少数である。日本では、かつて核融合特別推進研究の大規模なトリチウム生体影響研究班が組織され研究が行われていたが、研究組織の解体と同時に、トリチウム研究を継続する研究者は急激に減少した中での研究である。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (13 results)

All 2024 2023 2022 2021 2020

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (9 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results)

  • [Journal Article] Long Bones Exhibit Adaptive Responses to Chronic Low‐Dose‐Rate Ionizing Radiation despite its Lifespan‐Shortening and Carcinogenic Effects on <scp>C57BL</scp> /6 Mice2022

    • Author(s)
      Kohzaki Masaoki、Ootsuyama Akira、Abe Toshiaki、Tsukamoto Manabu、Umata Toshiyuki、Okazaki Ryuji
    • Journal Title

      JBMR Plus

      Volume: 6 Issue: 12 Pages: 1-13

    • DOI

      10.1002/jbm4.10688

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] STUDY OF BIOLOGICAL EFFECTS OF TRITIUM IN MICE2022

    • Author(s)
      Umata Toshiyuki
    • Journal Title

      Radiation Protection Dosimetry

      Volume: 198 Issue: 13-15 Pages: 1071-1076

    • DOI

      10.1093/rpd/ncac055

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparison of the fertility of tumor suppressor gene-deficient C57BL/6 mouse strains reveals stable reproductive aging and novel pleiotropic gene2021

    • Author(s)
      Kohzaki Masaoki、Ootsuyama Akira、Umata Toshiyuki、Okazaki Ryuji
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 12357-12369

    • DOI

      10.1038/s41598-021-91342-9

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] トリチウムの保険物理の最前線ートリチウムの生体影響研究ー2021

    • Author(s)
      馬田敏幸
    • Journal Title

      日本原子力学会誌ATOMOΣ

      Volume: 63 Pages: 723-727

    • Related Report
      2021 Research-status Report
  • [Presentation] Risk assessment of carcinogenesis due to exposure to tritiated water using a highly sensitive mouse model of carcinogenesis2024

    • Author(s)
      Toshiyuki Umata, Akira Ootsuyama, Megumi Sasatani
    • Organizer
      The 5th Workshop of the Research Center for Radiation Hazards and Medical Science
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Tritium water induces small intestinal tumors in Min mice2023

    • Author(s)
      Toshiyuki Umata, Akira Ootsuyama, Megumi Sasatani
    • Organizer
      日本放射線影響学会第66回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Analysis of tritium-induced gastrointestinal tumors in Min mouse2022

    • Author(s)
      馬田敏幸, 大津山彰
    • Organizer
      日本放射線影響学会第65回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Minマウスを用いたトリチウム誘発 小腸がんの解析2021

    • Author(s)
      馬田敏幸、大津山彰、笹谷めぐみ
    • Organizer
      第64回日本放射線影響学会
    • Related Report
      2021 Research-status Report
  • [Presentation] A Study of the Biological Effects of Tritium in Mice2021

    • Author(s)
      Toshiyuki Umata
    • Organizer
      International Symposium on Environmental Dynamics of Radionuclides and Biological Effects of Low Dose-Rate Radiation
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Analysis of tritium-induced gastrointestinal tumor in Min mouse2021

    • Author(s)
      Toshiyuki Umata, Akira Ootsuyama, Megumi Sasatani
    • Organizer
      The 94th Annual Meeting of the Japanese Biochemical Society
    • Related Report
      2021 Research-status Report
  • [Presentation] 遺伝子改変マウスを用いた トリチウムの生体影響研究2021

    • Author(s)
      馬田敏幸
    • Organizer
      日本原子力学会 2021年春の年会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 発がん高感受性モデルマウスを用いたトリチウム誘発がんの解析2020

    • Author(s)
      馬田 敏幸,大津山 彰,笹谷 めぐみ
    • Organizer
      第33回 バイオメディカル・ファジイ・システム学会
    • Related Report
      2020 Research-status Report
  • [Presentation] Analysis of tritium-induced gastrointestinal tumor in Min mouse2020

    • Author(s)
      Toshiyuki Umata, Akira Ootsuyama, Megumi Sasatani
    • Organizer
      第63回 日本放射線影響学会
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2025-01-30  

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