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Development of pesticide residue test kit based on synthetic organic chemistry and antibody engineering

Research Project

Project/Area Number 20K05583
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 34030:Green sustainable chemistry and environmental chemistry-related
Research InstitutionToyohashi University of Technology

Principal Investigator

Iwasa Seiji  豊橋技術科学大学, 工学(系)研究科(研究院), シニア研究員 (30303712)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords触媒 / 抗体 / 残留農薬検査 / 有機合成化学 / イムノアッセイ / イムノクロマト / キット / ハプテン / 有機合成 / 抗原抗体反応 / 残留農薬 / 検査キット / 遷移金属触媒 / カルベン移動反応 / 触媒的有機合成 / カルベン
Outline of Research at the Start

本研究は、抗原抗体反応に基づく分子認識機構を利用した免疫的化学測定法(イムノアッセイ)による果物や農産物の残留農薬分析のための簡便、廉価、迅速、高精度、自在な測定環境などの特徴を有するイムノクロマトキットを提供することを目的とする。特に現在、問題化しているリン酸エステル系やネオニコチノイド等の水溶性農薬を検査標的とする。技術的には触媒的合成技術を用いて標的農薬分子にリンカーを導入し、巨大蛋白と結合させてマウス免疫することで精度よく認識する標的特異的なモノクロナール抗体を作成する。得られる抗体の中で分析精度はppbレベルの標準としてキット化する。

Outline of Final Research Achievements

This research is characterized by simple, low-cost, rapid, high-precision, and flexible measurement environments for the analysis of pesticide residues in fruits and agricultural products by immunochemical assays (immunoassays) that utilize molecular recognition mechanisms based on antigen-antibody reactions. For the purpose of creating an immunochromatographic kit with Technically, a target-specific monoclonal antibody was created by introducing a linker into the target pesticide molecule using catalytic synthesis technology, binding it to a large protein, and immunizing mice, and made it into a kit. In the demonstration stage, a demonstration test was conducted at the collection point as a simple analytical method before shipment of cabbage. As a result, the immunochromatographic kit was simple, inexpensive, rapid, and had sufficient functions as a primary screening, and obtained practical results.

Academic Significance and Societal Importance of the Research Achievements

様々な環境負荷化学物質が健康や生態系に及ぼす影響が懸念されている。その手段として環境中の存在を常に監視するモニタリングが必要となる。ポジティブリスト制が施行され、全ての農薬の全ての作の残留基準値が設定されてから、作物への暴露や土壌残留による作物の汚染など想定していなかった農薬の残留の問題も表面化してきており、生産者の残留農薬への対応は必須となっている。環境及び食品の安全性を確保するには極微量の環境負荷化学物質を迅速・簡便かつ安価にしかも様々な場所で測定できる新しい技術開発が求められており、免疫化学測定法は有機溶剤などの多用による二次汚染のリスクが少なく有用な測定法として注目されている。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (23 results)

All 2022 2021 2020 Other

All Journal Article (11 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 11 results) Presentation (9 results) (of which Int'l Joint Research: 1 results) Remarks (3 results)

  • [Journal Article] Fluorocyclopropane-Containing Proline Analogue: Synthesis and Conformation of an Item in the Peptide Chemists Toolbox2022

    • Author(s)
      Amandine Pons , Jonathan Decaens , Riham Najjar , Nansalmaa Otog , Mathieu Arribat , Sandrine Jolly , Samuel Couve-Bonnaire , Muriel Sebban , Gael Coadou , Hassan Oulyadi , David Speybrouck , Seiji Iwasa, Andre B. Charette, Thomas Poisson, Philippe Jubault
    • Journal Title

      ACS Omega

      Volume: 7 Pages: 4868-4878

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Highly enantioselective synthesis of norcaradiene derivatives from naphthyl diazoacetamides using a Ru(<scp>ii</scp>)-Pheox complex2022

    • Author(s)
      Nansalmaa Otog , Bilguun Gantogos , Ikuhide Fujisawa , Seiji Iwasa
    • Journal Title

      Chemical Communications

      Volume: 58 Issue: 88 Pages: 12325-12328

    • DOI

      10.1039/d2cc04355f

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 残留農薬検査キットの開発 ー異分野先端技術の連携と融合ー2022

    • Author(s)
      岩佐精二
    • Journal Title

      バイオテック東海

      Volume: 2022

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Catalytic Asymmetric Carbene Insertion Reactions into B-H Bonds Using a Ru(II)-Pheox Complex2021

    • Author(s)
      Otog, N., Chanthamath, S.; Fujisawa, I.; Iwasa, S.
    • Journal Title

      Euro Journal of Organic Chemistry

      Volume: 2021 Issue: 10 Pages: 1564-1567

    • DOI

      10.1002/ejoc.202100034

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ru(II)‐Pheox Catalyzed Highly Stereoselective Cyclopropanation of Allyl‐ and Vinylsilanes with Diazoesters and Their Synthetic Applications2021

    • Author(s)
      Inoue, H.; Doan, T. T. T.; Batgerel, Z.; Langendorf, M, N.; Fujisawa, I.; Iwasa, S.
    • Journal Title

      ChemCatChem,

      Volume: 13 Issue: 1 Pages: 328-337

    • DOI

      10.1002/cctc.202001427

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Simultaneous Detection of Six Different Types of Pesticides by an Immunosensor Based on Surface Plasmon Resonance2020

    • Author(s)
      MIYAKE Shiro、HIRAKAWA Yuki、YAMASAKI Tomomi、WATANABE Eiki、HARADA Ayako、IWASA Seiji、NARITA Hiroshi
    • Journal Title

      Analytical Sciences

      Volume: 36 Issue: 3 Pages: 335-340

    • DOI

      10.2116/analsci.19P333

    • NAID

      130007806984

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2020-03-10
    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Immunoassay for Highly Water-soluble Nitenpyram: Evaluating the Analytical Performance of an Easy-to-use Screening Method for Agricultural Samples2020

    • Author(s)
      17.Eiki Watanabe; Yuki Hirakawa; Tomomi Yamasaki; Seiji Iwasa; Shiro Miyake, Eiki Watanabe
    • Journal Title

      Analytical Letters

      Volume: 52 Issue: 2 Pages: 174-187

    • DOI

      10.1080/00032719.2019.1642343

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Highly Efficient Synthesis of Oxindole Derivatives Via Catalytic Intramolecular C-H Insertion Reactions of Diazoamides2020

    • Author(s)
      Nga Phan Thi Thanh、Tone Masaya、Inoue Hayato、Shibatomi Kazutaka、Iwasa Seiji
    • Journal Title

      Key Engineering Materials

      Volume: 840 Pages: 251-256

    • DOI

      10.4028/www.scientific.net/kem.840.251

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] ynthesis of Forms of a Chiral Ruthenium Complex Containing a Ru-Colefin(sp2) Bond and Their Application to Catalytic Asymmetric Cyclopropanation Reactions2020

    • Author(s)
      Hayato Inoue, Nga Phan Thi Thanh, Ikuhide Fujisaw, Seiji Iwasa
    • Journal Title

      Organic Letters

      Volume: 22 Issue: 4 Pages: 1475-1479

    • DOI

      10.1021/acs.orglett.0c00050

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Synthesis of γ-lactams via Ru(II)?Pheox-catalyzed regioselective intramolecular Csp3?H insertion of diazoacetamides2020

    • Author(s)
      Fujii Takuji、Thu Huong Dang Thi、Iwasa Seiji
    • Journal Title

      Tetrahedron Letters

      Volume: 61 Issue: 37 Pages: 152276-152276

    • DOI

      10.1016/j.tetlet.2020.152276

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Synthesis of Oxindole Derivatives via Intramolecular C?H Insertion of Diazoamides Using Ru(II)-Pheox Catalyst2020

    • Author(s)
      Phan Thi Thanh Nga、Dang Thi Thu Huong、Tone Masaya、Inoue Hayato、Iwasa Seiji
    • Journal Title

      Tetrahedron

      Volume: 76 Issue: 43 Pages: 131481-131481

    • DOI

      10.1016/j.tet.2020.131481

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] ジアゾアミドの分子内C(sp3)-H挿入反応を経由する縮環型γ-ラクタム類の効率的な合成法の開発2021

    • Author(s)
      藤井 琢真,Dang Thi Thu Huong, 岩佐 精二
    • Organizer
      日本化学会第100春季年会
    • Related Report
      2021 Research-status Report
  • [Presentation] Catalytic Asymmetric Cyclopropanation Reactions of Diazo Oxime Ethers with Olefins and Their Synthetic Applications2021

    • Author(s)
      Nansalmaa Otog, Linh Da Ho, Iwasa, S.,
    • Organizer
      PacifiChem2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] アリルホウ素化合物への触媒的不斉シクロプロパン化反応2021

    • Author(s)
      小椋健司・岩佐精二
    • Organizer
      中化連第52回秋季大会
    • Related Report
      2021 Research-status Report
  • [Presentation] アリル-及びビニルシラン類の触媒的不斉シクロプロパン化反応とその応用2021

    • Author(s)
      OTOG NANSALMAA・井上颯斗・BATGEREL ZOLZAYA・岩佐精二
    • Organizer
      中化連第52回秋季大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 芳香環への触媒的不斉カルベン移動反応の開発2021

    • Author(s)
      GANTOGOS Bilguun・OTOG Nansalmaa ・岩佐精二
    • Organizer
      中化連第52回秋季大会
    • Related Report
      2021 Research-status Report
  • [Presentation] オレフィン類とジアゾオキシム化合物の触媒的不斉カルベン移動反応の開発2021

    • Author(s)
      BATGEREL ZOLZAYA・OTOG NANSALMAA・岩佐精二
    • Organizer
      中化連第52回秋季大会
    • Related Report
      2021 Research-status Report
  • [Presentation] Z-アルケニルジアゾカルボニル化合物の触媒的分子内不斉シクロプロパン化反応の開発2021

    • Author(s)
      Nengchue Oudomphone・山口剛・岩佐精二
    • Organizer
      中化連第52回秋季大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ジアゾアミドの分子内C(sp3)-H挿入反応を経由する縮環型γ-ラクタム類の効率的な合成法の開発2020

    • Author(s)
      藤井 琢真,Dang Thi Thu Huong, 岩佐 精二
    • Organizer
      日本化学会第100春季年会
    • Related Report
      2020 Research-status Report
  • [Presentation] イムノクロマト技術を用いたトマト中に残留する殺菌剤アゾキシストロビンの検出2020

    • Author(s)
      松永安由、西野友美、杉田和俊、三添英朗、横川武弘、 足立香代、岩佐精二、坂真智子、三宅司郎
    • Organizer
      第 43 回農薬残留分析研究会、日本農薬学会
    • Related Report
      2020 Research-status Report
  • [Remarks] Scopus

    • URL

      https://www.scopus.com/authid/detail.uri?authorId=7005881358

    • Related Report
      2022 Annual Research Report
  • [Remarks] Research Map

    • URL

      https://researchmap.jp/rupheox

    • Related Report
      2022 Annual Research Report
  • [Remarks] ResearchGate

    • URL

      https://www.researchgate.net/

    • Related Report
      2022 Annual Research Report

URL: 

Published: 2020-04-28   Modified: 2024-01-30  

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