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HPLC-ESR investigation to explore potent superoxide radical inhibiter involved in vegetables, fruits and beverages

Research Project

Project/Area Number 20K05937
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38050:Food sciences-related
Research InstitutionEhime University (2022)
Kyoto Institute of Technology (2020-2021)

Principal Investigator

Tajima Kunihiko  愛媛大学, 農学研究科, 研究員 (50163457)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsスーパーオキシドラジカル / HPLC-ESR / 電子スピン共鳴 / 抗酸化活性 / ESRクロマトグラム / 緑黄色野菜 / 水溶性成分 / 行者ニンニク
Outline of Research at the Start

本研究では、申請者が独 自に開発した HPLC-ESR 装置を用いて、着色性青果物の水溶性成分に含まれる着色成分と非着色 成分のスーパーオキシドラジカル(SOR)消去活性を分離して評価し、得られた知見から機能性青果物の品質と機能性を多角的に評価する基盤技術を確立する。そして、含着色成分機能性青果物に含まれる多数の機能性成分が複合的に作用して、青果物に由来する生物活性を発現する可能性を提案する。本研究の成果を基盤として、「科学的に保証された高機能性青果物の評価 技術」を農業界に提供することが本研究の最終的な目標である。

Outline of Final Research Achievements

The flow-spin-trapping ESR system combined with the HPLC apparatus (HPLC-ESR) enabled the observation of the ESR chromatograms of column eluates by post-column on-line ST-ESR detection of the DMPO/O2 radical. The current study demonstrates that the intensity and line shape of ESR chromatograms are dominated by SOR scavenging ability and concentration of antioxidants. Furthermore, the total SOR radical scavenging activities of the column-eluted fractions are accurately quantified using the equivalent concentration of caffeic acid ([CAeq.]). HPLC-ESR systems that allow visualization of SOR scavenging functions of column eluates are advantageous for exploring potential SOR scavengers contained in plant-derived water extracts. For example, HPLC-ESR analyses clarified that the most useful SOR scavenging active substances involved in coffee were chlorogenic acid derivatives and these contribution to the total SOR scavenging activity of coffee brew reached about 75%.

Academic Significance and Societal Importance of the Research Achievements

最近、優れたスパーオキシドラジカル(以下、SOR)消去活性を備えた青果物、果汁および飲料の積極的な摂取が健康維持に寄与することが広く認知され、SOR消去活性を指標とする青果物および関連食品の開発が続けられている。従来のSOR消去活性評価法では、青果物水溶性成分のSOR消去活性を混合物の状態で計測しているため、主要成分の濃度と活性については不明であった。本研究で確立したHPLC-ESR分析法では、青果物に含まれる主要成分をカラム分離し、それらのSOR消去活性のオンライン分析が可能であり、我が国の食品機能評価の分野への貢献が期待できる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (10 results)

All 2022 2021 2020

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 4 results) Presentation (2 results)

  • [Journal Article] Stopped-flow-optical Absorption and -ESR Detection of Rutin (Quercetin-3-O-rutinoside) B-ring Catechol Aroxyl Radicals Generated during Redox Reaction between Rutin and superoxide Radical in DMSO2022

    • Author(s)
      Kuwabara, Keiko; Nishio, Natsuki; Nakano, Ryota; Sakurai, Yasuhiro; Yamaguchi, Tomoko; Miyake, Yusuke; Kanaori, Kenji; Tajima, Kunihiko
    • Journal Title

      Chemistry Letters

      Volume: 51 Issue: 4 Pages: 431-434

    • DOI

      10.1246/cl.220009

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Systematic Study of Pnictogen-Fused Heterofluorenes2022

    • Author(s)
      Inaba Ryoto、Oka Kouki、Iwami Takahiro、Miyake Yusuke、Tajima Kunihiko、Imoto Hiroaki、Naka Kensuke
    • Journal Title

      Inorganic Chemistry

      Volume: 61 Issue: 19 Pages: 7318-7326

    • DOI

      10.1021/acs.inorgchem.2c00158

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Watanabe, Seiyaa, b, cSend mail to Watanabe S.; Murase, Yohsukea; Watanabe, Yasunoria, b, d; Sakurai, Yasuhiroe; Tajima, Kunihikoe2022

    • Author(s)
      Watanabe, Seiyaa, Murase, Yohsukea; Watanab, Yasunori, Sakurai, Yasuhiro; Tajima, Kunihiko
    • Journal Title

      Communications BiologyO

      Volume: 4 Issue: 1 Pages: 681-681

    • DOI

      10.1038/s42003-021-02147-5

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Flow spin-trapping esr detection of OH and H radicals derived from helium atmospheric-pressure plasma at gasliquid interface employing a micro open-flow reactor2021

    • Author(s)
      Sakurai, Yasuhiro, Yamamoto, Naoko; Yabuta, Yuki; Kanaori, Kenji; Tajima, Kunihiko
    • Journal Title

      Chemistry Letters

      Volume: 50 Issue: 9 Pages: 1628-1631

    • DOI

      10.1246/cl.210282

    • NAID

      130008088197

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mechanism of 2,6-dichloro-4,4'-bipyridine-catalyzed diboration of pyrazines involving a bipyridine-stabilized boryl radical2021

    • Author(s)
      Ohmura, Toshimichia; Morimasa, Yohei; Ichino, Tomoya; Miyake, Yusuke; Murata, Yasujiro; Suginome, Michinori; Tajima, Kunihiko; Taketsugu, Tetsuya, Maeda, Satoshi,
    • Journal Title

      Bulletin of Chemical Society Japan

      Volume: 90 Issue: 7 Pages: 1894-1902

    • DOI

      10.1246/bcsj.20210145

    • NAID

      130008076884

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Stopped-flow-optical Absorption and -electron Spin Resonance Studies on Short-lived Quercetin Semiquinone Radical Produced by Redox Reactions with O2-・ Radical in DMSO,2020

    • Author(s)
      K. Kuwabara, R. Nakano, Y. Sakurai, T. Yamaguchi, Y. Miyake, K. Kanaori, and K. Tajima
    • Journal Title

      Chem. Lett.

      Volume: 49 Issue: 3 Pages: 260-263

    • DOI

      10.1246/cl.190898

    • NAID

      130007805924

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of Flow-Injection Spin-Trapping ESR Methods for0 Kinetic Study of the O2-・ Radical Scavenging Reaction with Mixtures of Phenolic Antioxidants,2020

    • Author(s)
      Y. Sakurai, S. Yamaguchi, N. Yamamoto, T. Yamashita, Y. Lu, K. Kuwabara, T. Yamaguchi, Y. Miyake, K. Kanaori, and K. Tajima,
    • Journal Title

      Bull. Chem. Soc. Jpn.

      Volume: 93 Issue: 6 Pages: 730-744

    • DOI

      10.1246/bcsj.20200046

    • NAID

      130007856027

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Nucleobase-involved native chemical ligation: a novel reaction between an oxanine nucleobase and N-terminal cysteine for oligonucleotide-peptide conjugation2020

    • Author(s)
      Eui Kyoung Jang, Yohei Koikeb Yuko Ide, Kunihiko Tajima, Kenji Kanaori and Seung Pil Pack
    • Journal Title

      Chemical Communications

      Volume: 56 Issue: 41 Pages: 5508-5511

    • DOI

      10.1039/c9cc08808c

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 温度可変ストップト ESR 法によるスーパーオキシドラジカルとガロカテキンの酸化還元反応系における 短寿命 B 環ピロガロールセミキノンラジカルの構造2022

    • Author(s)
      田嶋邦彦、中野涼汰、桑原慶子、櫻井康博、三宅祐輔、金折賢二
    • Organizer
      電子スピンサイエンス学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] スーパーオキシドとフラボノイドの酸化還元反応機構の構造化学的研究2022

    • Author(s)
      桑原慶子、田嶋邦彦、櫻井康博、三宅祐輔、金折賢二
    • Organizer
      電子スピンサイエンス学会
    • Related Report
      2022 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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