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Cell wall homeostasis mechanisms involved in mechanical optimisation during gravity response.

Research Project

Project/Area Number 20K05950
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38060:Applied molecular and cellular biology-related
Research InstitutionUniversity of Tsukuba

Principal Investigator

Iwai Hiroaki  筑波大学, 生命環境系, 准教授 (30375430)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords細胞壁 / 重力 / 力学的特性 / ペクチン / 重力屈性 / 細胞壁構造タンパク質 / マトリックス多糖類 / 重力応答
Outline of Research at the Start

本研究課題の核心は、植物が重力応答時に、どのような細胞壁成分が少なくなり、また多くなることで力学的最適化を行う「細胞壁ホメオスタシス」機構が存在するかという謎に対し、細胞壁改変イネを用いた倒伏回復時の細胞壁の力学的性質を回復する過程を調べることで解決に挑む。戦略として(1)倒伏回復に異常が生じた細胞壁改変イネの解析により、すでに有力な結果が出ている候補について調査する、(2)細胞壁改変イネおよび熱帯植物ライブラリーからの新規の重力応答性細胞壁マーカーの探索、(3)重力応答予測数理モデルの構築することにより、新たな重力応答予測モデルの構築を目指す。

Outline of Final Research Achievements

Gravity has been used by plants as a source of information to maintain their own normal position. From more than 40 rice species with altered pectin, hemicellulose and cell wall structural proteins, we sought to identify rice plants with altered gravitropism. The results showed that the angle of recovery from the collapsed state was about 46% higher in xylosidase-overexpressing rice plants and about 10% lower in β-xylanase-overexpressing rice plants. The study also investigated whether pectin, which determines the mechanical properties of the pollen tube pathway or the pollen column, is responsible for regulating fertilization as a physical barrier. Mutants of pectin methyltransferase caused abnormalities in the mechanical properties of the pollen column of the pistil, resulting in the failure of the pollen tube to penetrate.

Academic Significance and Societal Importance of the Research Achievements

新たな重力応答性細胞壁マーカーの同定は本研究の独創的な点であり、他の環境応答バイオマーカーとの技術融合による建材植物生産効率の大幅向上が期待できる。また、タケやヤシ科植物など、他の建築用資材植物の有料資材獲得の大幅効率化にも寄与できると予想される。これらの成果を元に、重力応答数理予測モデルを構築することは、異分野融合に大きな貢献をするものと期待している。また、重力応答数理予測モデルは、適切な材料を選択することも含め建築設計にも利用できるものを構築したいと考えている。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (12 results)

All 2022 2021 2020

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] Effects of polygalacturonase overexpression on pectin distribution in the elongation zones of roots under aluminium stress2022

    • Author(s)
      Nagayama Teruki、Tatsumi Akane、Nakamura Atsuko、Yamaji Naoki、Satoh Shinobu、Furukawa Jun、Iwai Hiroaki
    • Journal Title

      AoB PLANTS

      Volume: 14 Issue: 2

    • DOI

      10.1093/aobpla/plac003

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Virtual issue: cell wall functions in plant growth and environmental responses2021

    • Author(s)
      Iwai Hiroaki
    • Journal Title

      Journal of Plant Research

      Volume: 134 Issue: 6 Pages: 1155-1158

    • DOI

      10.1007/s10265-021-01351-y

    • NAID

      40022724712

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural Alternation of Rice Pectin Affects Cell Wall Mechanical Strength and Pathogenicity for the Rice Blast Fungus under Weak Light Conditions2021

    • Author(s)
      Ohara T, Takeuchi H, Sato J, Nakamura A, Ichikawa H, Yokoyama R, Nishitani K, Minami E, Satoh S, Iwai H.
    • Journal Title

      Plant & Cell Physiology

      Volume: - Issue: 4 Pages: 641-649

    • DOI

      10.1093/pcp/pcab019

    • Related Report
      2021 Research-status Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rice Putative Pectin Methyltransferase Gene OsPMT10 Is Required for Maintaining the Cell Wall Properties of Pistil Transmitting Tissues via Pectin Modification2021

    • Author(s)
      Hasegawa Kazuya、Kamada Shihomi、Takehara Shohei、Takeuchi Haruki、Nakamura Atsuko、Satoh Shinobu、Iwai Hiroaki
    • Journal Title

      Plant and Cell Physiology

      Volume: 62 Issue: 12 Pages: 1902-1911

    • DOI

      10.1093/pcp/pcab078

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cell wall Glycine-rich Protein2 is involved in tapetal differentiation and pollen maturation.2020

    • Author(s)
      Takebe N, Nakamura A, Watanabe T, Miyashita A, Satoh S, Iwai H.
    • Journal Title

      J Plant Res.

      Volume: 133 Issue: 6 Pages: 883-895

    • DOI

      10.1007/s10265-020-01223-x

    • NAID

      40022411372

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rice Putative Methyltransferase Gene OsPMT16 Is Required for Pistil Development Involving Pectin Modification.2020

    • Author(s)
      Hasegawa K, Kamada S, Takehara S, Takeuchi H, Nakamura A, Satoh S, Iwai H.
    • Journal Title

      Front Plant Sci.

      Volume: 11 Pages: 475-475

    • DOI

      10.3389/fpls.2020.00475

    • NAID

      120007166912

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 環境ストレス応答における植物細胞壁ペクチンの機能2022

    • Author(s)
      岩井 宏暁
    • Organizer
      第133回 日本森林学会大会  生理部門企画シンポジウム「動的な細胞壁」
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 塩ストレス条件下の早期果実形成過程における細胞壁の変化2021

    • Author(s)
      矢野朱華; 板野愛都; 宮越茜里; 佐藤忍; 岩井 宏暁
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 根の伸長におけるイネ細胞壁タンパク質 THRGP の機能解析2021

    • Author(s)
      木田朋宏; 大場裕介; 渡辺朝美; 宮下彩; 中村敦子; 佐藤忍; 岩井 宏暁
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 塩ストレス条件下でのトマト成熟果実における維管束構築の変化2021

    • Author(s)
      小坂友紀; 高橋玲奈; 杉山晴香; 沼尻侑子; 宇賀優作; 佐藤忍; 岩井 宏暁
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] イネにおける細胞壁多糖類のアルミニウム適応応答2021

    • Author(s)
      長山 照樹,斎藤 大夢,中村 敦子,山地 直樹,佐藤 忍,古川 純,岩井 宏暁
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] 植物細胞壁と金属とのお付き合い ~力学的特性の変化~2020

    • Author(s)
      岩井宏暁
    • Organizer
      バイオeカフェ
    • Related Report
      2020 Research-status Report
    • Invited

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Published: 2020-04-28   Modified: 2024-01-30  

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