• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Novel function of FAB1 in secondary cell wall element secretion and cortical microtubule polymerisation

Research Project

Project/Area Number 20K05962
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38060:Applied molecular and cellular biology-related
Research InstitutionKyoto Prefectural University

Principal Investigator

Tomoko Hirano  京都府立大学, 生命環境科学研究科, 准教授 (20724496)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsFAB1 / 根毛 / 二次細胞壁成分の輸送 / 後期エンドソーム / 表層微小管 / PI(3,5)P2 / KINESIN12 / 表層微小管の重合 / チューブリンのフォールディング / 植物細胞の形態形成 / Prefoldin / 二次細胞壁成分の分泌
Outline of Research at the Start

根毛,花粉管,トライコーム,気孔と表皮細胞など複雑で特殊な形態をもつ植物細胞の形態形成のために必須な三大要素は,細胞骨格である「アクチン」と「表層微小管」の配向,及び「二次細胞壁」の形成である.このうち,「表層微小管の重合」と「二次細胞壁成分の分泌」の両者を同時に制御する分子がホスファチジル3リン酸5キナーゼ,FAB1である.しかし,「FAB1が,どのように表層微小管の重合を助けているか?」そして,「FAB1が形成するエンドソームに積み荷された二次細胞壁成分がどのようなモータータンパク質によって,どのように運ばれているか?」が不明である.本研究では,この問いを明らかにする.

Outline of Final Research Achievements

The three essential elements for the complex and specialised morphogenesis of plant cells such as root hairs, pollen tubes, trichomes, stomata and epidermal cells are the orientation of the cytoskeleton 'actin' and 'cortical microtubules', and the formation of 'secondary cell walls'. We found that phosphatidyl 3-phosphate 5-kinase, FAB1, is a molecule that simultaneously regulates both the polymerisation of cortical microtubules and the secretion of secondary cell wall components. However, "how does FAB1 assists in the microtubule polymerisation?" and "how the secondary cell wall components are secreted by the FAB1-endosomes?" remained unknown. This study elucidates the secretion mechanism of secondary cell wall components.

Academic Significance and Societal Importance of the Research Achievements

植物の細胞壁のうち,すべての細胞に存在する一次細胞壁は,柔軟性を持ち,細胞が成長する過程で拡大・伸長し,細胞のかたち作りを様々な機構で制御している.これに対し,二次細胞壁は,植物や植物細胞の形を維持したり,硬さや頑強さを与えたりする役割をもつ.したがって,植物の細胞分裂や形態形成において中心的に働く微小管の生成機構や,植物に頑強さを与え,かつ,伸長抑制に働くことで,形態形成を担う二次細胞壁の形成過程・機構を理解することは,真に植物の成長を理解することである.
また,我々が資源としている木質バイオマスの大半が二次細胞壁に由来するため,本研究は,応用的側面にも大きな意義も持つ.

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (8 results)

All 2023 2022 2021 2020

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (3 results) (of which Invited: 3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Ab-GALFA, A bioassay for insect gall formation using the model plant Arabidopsis thaliana2023

    • Author(s)
      Tomoko Hirano , Ayaka Okamoto , Yoshihisa Oda , Tomoaki Sakamoto , Seiji Takeda , Takakazu Matsuura , Yoko Ikeda , Takumi Higaki , Seisuke Kimura , Masa H Sato
    • Journal Title

      Scientific Reports

      Volume: 13 Issue: 1 Pages: 2554-2554

    • DOI

      10.1038/s41598-023-29302-8

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recent Progress Regarding the Molecular Aspects of Insect Gall Formation.2021

    • Author(s)
      Takeda S, Hirano T, Ohshima I, Sato MH.
    • Journal Title

      Int J Mol Sci.

      Volume: 22 Issue: 17 Pages: 9424-9435

    • DOI

      10.3390/ijms22179424

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Reprogramming of the Developmental Program of Rhus javanica During Initial Stage of Gall Induction by Schlechtendalia chinensis.2020

    • Author(s)
      Hirano T, Kimura S, Sakamoto T, Okamoto A, Nakayama T, Matsuura T, Ikeda Y, Takeda S, Suzuki Y, Ohshima I, Sato MH.
    • Journal Title

      Front Plant Sci.

      Volume: 11:471 Pages: 1-13

    • DOI

      10.3389/fpls.2020.00471

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Callose Synthesis Suppresses Cell Death Induced by Low-Calcium Conditions in Leaves2020

    • Author(s)
      Shikanai Yusuke、Yoshida Ryosuke、Hirano Tomoko、Enomoto Yusuke、Li Baohai、Asada Mayu、Yamagami Mutsumi、Yamaguchi Katsushi、Shigenobu Shuji、Tabata Ryo、Sawa Shinichiro、Okada Hiroki、Ohya Yoshikazu、Kamiya Takehiro、Fujiwara Toru
    • Journal Title

      Plant Physiology

      Volume: 182 Issue: 4 Pages: 2199-2212

    • DOI

      10.1104/pp.19.00784

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 「虫こぶ」形成の謎は解明しつつある2022

    • Author(s)
      平野朋子
    • Organizer
      日本植物生理学会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] おいしい家をつくる 虫のすごい能力を探る2021

    • Author(s)
      平野朋子
    • Organizer
      新自然史科学創成センターシンポジウム
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 昆虫が植物を改変させて起こす 寄生・共生現象「虫こぶ」の謎に迫る2021

    • Author(s)
      平野朋子
    • Organizer
      日本共生生物学会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] 植物幹細胞誘導作用及び植物病害抵抗性誘導作用を有するペプチド2020

    • Inventor(s)
      佐藤雅彦,平野朋子,大島一正,木村成介
    • Industrial Property Rights Holder
      佐藤雅彦,平野朋子,大島一正,木村成介
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2020 Research-status Report

URL: 

Published: 2020-04-28   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi