New breeding strategy using interaction between plants and endosphere bacteria

• Project/Area Number: 20K05982
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: Osaka Kyoiku University
• Principal Investigator: Suzuki Go 大阪教育大学, 教育学部, 教授 (10314444)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: マイクロバイオーム / エンドファイト / イネ / Endosphere / 根組織内バクテリア / FISH法 / 植物-微生物間相互作用

Outline of Research at the Start

本研究では『マイクロバイオーム解析』と『植物組織内バクテリア局在可視化』を両輪に、エンドファイト群集の基盤情報を蓄積する。様々な環境下・生育状態のイネやトマト根組織内マイクロバイオームを解析し、エンドファイト群集として『植物の生育状況』と『バクテリア組織内局在』が連関しているバクテリア種を探索・同定する。さらには、この植物-微生物間相互作用に関係する植物側受容因子を遺伝学的に精査し、持続的農業に寄与する新しい育種戦略を構築する。

Outline of Final Research Achievements

In this study, we used next-generation sequencing to understand the microbiome in the root tissues that affect the growth of rice and tomato. We also visualized the localization of the bacteria in the root tissues by bacterial FISH analysis. Furthermore, we tried to establish a PCR analysis platform to identify genetic factors that incorporate endophytes into the plants. We comprehensively analyzed the Japanese rice endosphere microbiome data using statistical analysis and compared the results with the Malaysian data. This research paper has been published as the result of international collaborative research with Malaysian collaborators.

Academic Significance and Societal Importance of the Research Achievements

学述的には、本研究成果によって植物と共生菌の相互作用を包括的に理解することができ、イネにおいてはマレーシアと日本で地域が違うなかでの根組織内バクテリアの共通性などを明らかにしたことが意義深い。また、PCRを用いてバクテリア量を決定する手法を確立し、遺伝学的解析を行うための基礎を築くことができた。社会的意義としては、今後の次世代型持続的農業のためにエンドファイトを最大限利用できるイネの育成に向けて
第一歩を踏み出すことができ、またトマト栽培におけるコンパニオンプランツの役割をエンドファイトの面から理解することで、新しい持続的農業へ寄与できる可能性を示した。

Research Products

• [Int'l Joint Research] モナシュ大学マレーシア(マレーシア)
• [Int'l Joint Research] モナシュ大学マレーシア(マレーシア)
• [Int'l Joint Research] モナシュ大学マレーシア(マレーシア)
• [Int'l Joint Research] モナシュ大学マレーシア(マレーシア)
• [Journal Article] Comparative analysis of root associated microbes in tropical cultivated and weedy rice (Oryza spp.) and temperate cultivated rice. (2024)
  • Author(s): Juliyanti, V., Itakura, R., Kotani, K., Lim, S.Y., Suzuki, G., Chong, C.W., Song, B.K., Rahman, S.
  • Journal Title: Scientific Reports Volume: 14 Issue: 1 Pages: 9656-9656
  • DOI: 10.1038/s41598-024-60384-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Spatiogenetic characterization of <i>S</i> receptor kinase (<i>SRK</i>) alleles in naturalized populations of <i>Raphanus sativus</i> L. var. <i>raphanistroides</i> on Yakushima island (2021)
  • Author(s): Fukushima, K., Kanomata, T., Kon, A., Masuko-Suzuki, H., Ito, K., Ogata, S., Takada, Y., Komatsubara, Y., Nakamura, T., Watanabe, T., Koizumi, S., Sanuki, H., Park, J.-I., Niikura, S., Suwabe, K., Fujii, S., Murase, K., Takayama, S., Suzuki, G., and Watanabe, M.
  • Journal Title: Genes & Genetic Systems Volume: 96 Issue: 3 Pages: 129-139
  • DOI: 10.1266/ggs.20-00066
  • NAID: 130008102461
  • ISSN: 1341-7568, 1880-5779
  • Year and Date: 2021-06-01
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genetic diversity of genes controlling unilareral incompatibility in Japanese cultivars of Chinese cabbage. (2021)
  • Author(s): Takada, Y., Mihara, Y., He, Y., Xie, H., Ozaki, Y., Nishida, H., Hong, S., Lim, Y. P., Takayama, S., Suzuki, G., and Watanabe, M.
  • Journal Title: Plants Volume: 10 Issue: 11 Pages: 2467-2467
  • DOI: 10.3390/plants10112467
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of self-incompatible <i>Brassica napus</i> lines lacking <i>SP11</i> expression (2020)
  • Author(s): Okamoto, T., Okamoto, M., Hikichi, E., Ogawa, M., Takada, Y., Suzuki, G., Takayama, S., and Watanabe, M.
  • Journal Title: Genes & Genetic Systems Volume: 95 Issue: 3 Pages: 111-118
  • DOI: 10.1266/ggs.19-00050
  • NAID: 130007891310
  • ISSN: 1341-7568, 1880-5779
  • Year and Date: 2020-06-01
  • Peer Reviewed / Open Access
• [Journal Article] Double-locking mechanism of self-compatibility in Arabidopsis thaliana: the synergistic effect of transcriptional depression and disruption of coding region in the male specificity gene. (2020)
  • Author(s): Suwabe, K., Nagasaka, K., Windari, E. A., Hoshiai, C., Ota, T., Takada, M., Kitazumi, A., Masuko-Suzuki, H., Kagaya, Y., Yano, K., Tsuchimatsu, T., Shimizu, K. K., Takayama, S., Suzuki, G., and Watanabe, M.
  • Journal Title: Frontiers in Plant Science Volume: 11 Pages: 576140-576140
  • DOI: 10.3389/fpls.2020.576140
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mechanism of self/nonself- discrimination in Brassica self-incompatibility. (2020)
  • Author(s): Murase, K., Moriwaki, Y., Mori, T., Xiao, L., Masaka, C., Takada, Y., Maesaki, R., Mishima, M., Fujii, S., Hirano, Y., Kawabe, Z., Nagata, K., Terada, T., Suzuki, G., Watanabe, M., Shimizu, K., Hakoshima, T., and Takayama, S.
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 4916-4916
  • DOI: 10.1038/s41467-020-18698-w
  • Peer Reviewed / Open Access / Int'l Joint Research