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Effective control of light condition for production of a crude drug 'Hange', a dried tuber of Pinellia ternate, and elucidation of its genetic variation grown in Japan naturally

Research Project

Project/Area Number 20K06322
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 41040:Agricultural environmental engineering and agricultural information engineering-related
Research InstitutionKyushu University

Principal Investigator

Eguchi Toshihiko  九州大学, 実験生物環境制御センター, 准教授 (40213540)

Co-Investigator(Kenkyū-buntansha) 田中 宏幸  山陽小野田市立山口東京理科大学, 薬学部, 教授 (30253470)
尾崎 行生  九州大学, 農学研究院, 教授 (60253514)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords光環境制御 / 薬用植物 / 生産性 / 遺伝的変異 / 休眠 / 育種
Outline of Research at the Start

生薬「半夏(ハンゲ)」は,日本で使用されている漢方薬の約 23%に配合される重要生薬で,カラスビシャクの塊茎を原料とする.本種は国内全域に自生しているものの,半夏の供給は全てを中国からの輸入に頼っている.本課題は,カラスビシャクの栽培植物化・国内生産を目指した研究の一環として,植物の生産性や物質蓄積に大きく影響するとされる光条件に着目し,ハンゲの効率的生産のための至適光条件の探索を行い,また本種の効率的な育種のために日本自生カラスビシャクの遺伝的変異を明らかにする.

Outline of Final Research Achievements

In this study, we aimed to examine the optimal conditions for Pinellia ternata Breit. (‘Karasu-bishaku’ in Japanese) cultivation which is the raw material plant of the crude drug “Hange'', and to investigate the genetic background of the plants naturally grown in Japan for efficient breeding.
Corm growth was promoted under blue light, and the content of the effective ingredients (araban) in corm increased under green light. In addition, it was suggested that there are 6- to 9-ploids in the Japanese native plants. Furthermore, we confirmed that the Japanese native plants have mutations in the chloroplast DNA, the mutations which were not found in the Chinese and Korean native strains.

Academic Significance and Societal Importance of the Research Achievements

生薬「半夏(ハンゲ)」の原料植物カラスビシャクは日本国内全域に自生しているにもかかわらず,半夏の供給は全て中国からの輸入に頼っており,将来,供給量の減少や価格高騰により国内需要への対応が困難となると予想される.そこで,我々はカラスビシャクの栽培植物化・国内生産を目指した研究に着手し,本研究では,カラスビシャク球茎(ハンゲ原料)の肥大・品質に及ぼす栽培時の光環境について調査し,球茎肥大は青色光下で促進され,球茎の有用成分(アラバン)含量は緑色光下で高まることを明らかにした.また,本種の育種(優良系統のかけあわせ)において重要となる倍数性の変異を調査し,6倍体から9倍体が存在することが示唆された.

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (8 results)

All 2023 2022 2020

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (3 results)

  • [Journal Article] Drying method affects sugar content in the corm of the medicinal plant Pinellia ternata Breit.2023

    • Author(s)
      Eguchi, T., Igarashi, K., Sato, H., Yoshida, S., Matsuoka, K.
    • Journal Title

      Environmental Control in Biology

      Volume: 61

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Time-course Pattern of Growth of the Medicinal Plant <i>Pinellia ternata</i> Breit. Grown under Controlled Environments2022

    • Author(s)
      Eguchi, T., Tanaka, H., Sagawa, M., Yoshida, S., Matsuoka, K.
    • Journal Title

      Environment Control in Biology

      Volume: 60 Issue: 4 Pages: 191-194

    • DOI

      10.2525/ecb.60.191

    • ISSN
      1880-554X, 1883-0986
    • Year and Date
      2022-10-01
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of Nutrient Solution Electrical Conductivity and pH on the Productivity of the Medicinal Plant <i>Pinellia ternata</i> Breit.2022

    • Author(s)
      Eguchi, T., Tanaka, H., Yoshida, S., Matsuoka, K.
    • Journal Title

      Environment Control in Biology

      Volume: 60 Issue: 2 Pages: 149-151

    • DOI

      10.2525/ecb.60.149

    • ISSN
      1880-554X, 1883-0986
    • Year and Date
      2022-04-01
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Temperature Effects on the Photosynthesis by the Medicinal Plant <i>Pinellia ternata</i> Breit2020

    • Author(s)
      Toshihiko EGUCHI, Hiroyuki TANAKA, Daichi MORIUCHI, Satoshi YOSHIDA and Ken MATSUOKA
    • Journal Title

      Environment Control in Biology

      Volume: 58 Issue: 2 Pages: 49-50

    • DOI

      10.2525/ecb.58.49

    • NAID

      130007834349

    • ISSN
      1880-554X, 1883-0986
    • Year and Date
      2020-04-01
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 水溶性多糖悩含有カラスビシャク(九大たまゆたか)の鎖嘔吐作用の評価2020

    • Author(s)
      田中宏幸,楠瀬直喜,江口壽彦,吉田 敏,松岡 健
    • Journal Title

      山陽小野田市立山口東京理科大学紀要

      Volume: 3 Pages: 37-39

    • NAID

      120006844710

    • Related Report
      2020 Research-status Report
    • Open Access
  • [Presentation] 生薬半夏の有効多糖アラバンを認識するモノクローナル抗体のエピトープ探索2022

    • Author(s)
      佐藤春菜, 栗木淳寛, 田中宏幸, 江口壽彦, 松岡 健
    • Organizer
      日本農芸化学会 2022 年度大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 薬用植物カラスビシャク球茎の生育と有用成分蓄積に及ぼす光質の影響2022

    • Author(s)
      江口壽彦,田中宏幸,濱島里佳,栗木淳寛,吉田敏,松岡健
    • Organizer
      日本生物環境工学会2022年福岡大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 収穫後の処理が生薬‘半夏’の品質に及ぼす影響2022

    • Author(s)
      五十嵐健真,田中宏幸,松岡 健,吉田 敏,江口壽彦
    • Organizer
      日本生物環境工学会九州支部2022年熊本大会
    • Related Report
      2022 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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