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Novel cryodevices for vitrification of cumulus-oocyte complexes and pancreatic islets

Research Project

Project/Area Number 20K06364
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 42010:Animal production science-related
Research InstitutionShinshu University

Principal Investigator

Hochi Shinichi  信州大学, 学術研究院繊維学系, 教授 (10283243)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2023: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsガラス化保存 / ウシ卵丘卵子複合体 / ラット膵島 / シルクフィブロイン / シルクフィブロインスポンジ / 膵島移植 / ラット / VEGF / 脱細胞化組織 / スフェロイド培養 / 偽膵島 / ガラス化デバイス / 卵丘卵子複合体 / 膵ランゲルハンス島
Outline of Research at the Start

生体適合性の高い素材を用いてガラス化保存の新規デバイス開発を目指す対象材料は、ウシの卵丘卵子複合体とラットの膵ランゲルハンス島で、いずれも主役を演じる細胞 (卵子、β細胞) 以外にも多種多様な機能をもつ細胞群から構成され、それらが協同して働くことを特徴とする細胞塊である。本研究では、冷却前後の諸操作を簡便化できる新規デバイスをシルクなどの生体適合性の高い材料から作製・加工し、これらの細胞塊のガラス化保存に適用する。

Outline of Final Research Achievements

The high biocompatibility and easy processing capability of silk fibroin (SF) can facilitate successful scaffold design in biomedical research and practical applications, including cell transplantation therapy. The porous SF sponge with high hydrophilicity also serves as a transporter in drug delivery system because the SF sponge is suitable for absorption, retention, and gradual release of pharmaceutical aqueous solutions. Furthermore, the cryodevice derived from the SF sponge can be optimized for minimum volume cooing vitrification of large cell complexes or tissues composed of different cell types (e.g., rat pancreatic islets or bovine cumulus-oocyte complexes). This system requires prompt minimization of exposure to the highly toxic vitrification solution around the cell masses immediately before cooling in liquid nitrogen to guarantee the extremely rapid cooling rate and high post-warm cell viability.

Academic Significance and Societal Importance of the Research Achievements

ガラス化保存の新規デバイス開発を目指す対象材料はウシの卵丘卵子複合体とラットの膵島であり、いずれも主役の細胞 (卵子、β細胞) 以外にも多種多様な機能をもつ細胞群から構成され、それらが協同して働くことを特徴とする細胞塊である。これらのガラス化保存に適したデバイスを加工性・吸水性・生体適合性に優れた多孔質のシルクフィブロイン (SF) から作製できた。ウシ卵丘卵子複合体には市販クライオトップにSFシートを多層に巻き付け、ラット膵島にはSFスポンジをディスク上に切り抜き、それらの有用性を実証した。これらの成果は、ヒトの不妊治療や糖尿病の移植医療に関わる研究者・技術者に貢献する、と期待される。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (18 results)

All 2023 2022 2021 2020

All Journal Article (11 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 11 results,  Open Access: 7 results) Presentation (6 results) Book (1 results)

  • [Journal Article] Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells2023

    • Author(s)
      Iwatsuki Kenyu、Oikawa Mami、Kobayashi Hisato、Penfold Christopher A.、Sanbo Makoto、Yamamoto Takuya、Hochi Shinichi、Kurimoto Kazuki、Hirabayashi Masumi、Kobayashi Toshihiro
    • Journal Title

      Cell Reports Methods

      Volume: 3 Issue: 8 Pages: 100542-100542

    • DOI

      10.1016/j.crmeth.2023.100542

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Knockdown of Y-box binding protein 1 induces autophagy in early porcine embryos2023

    • Author(s)
      Jiang Wen-Jie、Lee Song-Hee、Heo Geun、Chung Hak Jae、Cho Eun Seok、Sa Soo Jin、Hochi Shinichi、Cui Xiang-Shun
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 11 Pages: 1238546-1238546

    • DOI

      10.3389/fcell.2023.1238546

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Characterization of homozygous Foxn1 mutations induced in rat embryos by different delivery forms of Cas9 nuclease2023

    • Author(s)
      Goto Teppei、Yogo Kyoko、Hochi Shinichi、Hirabayashi Masumi
    • Journal Title

      Molecular Biology Reports

      Volume: 50 Issue: 2 Pages: 1231-1239

    • DOI

      10.1007/s11033-022-08054-0

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM102023

    • Author(s)
      Kim Yong-Min、Park Sung-Woo、Lee Mi-Jin、Jeon Da-Yeon、Sa Su-Jin、Jeong Yong-Dae、Seong Ha-Seung、Choi Jung-Woo、Hochi Shinichi、Cho Eun-Seok、Chung Hak-Jae
    • Journal Title

      Journal of Animal Science and Technology

      Volume: 65 Pages: 401-411

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection2022

    • Author(s)
      Hochi Shinichi、Ide Misuzu、Ueno Sayaka、Hirabayashi Masumi
    • Journal Title

      Journal of Reproduction and Development

      Volume: 68 Issue: 5 Pages: 335-339

    • DOI

      10.1262/jrd.2022-053

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cryodevices developed for minimum volume cooling vitrification of bovine oocytes2022

    • Author(s)
      Hochi Shinichi
    • Journal Title

      Animal Science Journal

      Volume: 93 Issue: 1

    • DOI

      10.1111/asj.13683

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acellular matrix derived from rat liver improves the functionality of rat pancreatic islets before or after vitrification2021

    • Author(s)
      Nakayama-Iwatsuki Kenyu、Yanagisawa Kotaro、Tanaka Dan、Hirabayashi Masumi、Negishi Jun、Hochi Shinichi
    • Journal Title

      Cryobiology

      Volume: 100 Pages: 90-95

    • DOI

      10.1016/j.cryobiol.2021.03.005

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] All-in-One Silk Fibroin Sponge as the Vitrification Cryodevice of Rat Pancreatic Islets and the VEGF-Embedded Scaffold for Subrenal Transplantation2021

    • Author(s)
      Yamanaka Takahiro、Nakayama-Iwatsuki Kenyu、Fujimoto Sora、Hirono Naoki、Negishi Jun、Tamada Yasushi、Hirabayashi Masumi、Hochi Shinichi
    • Journal Title

      Transplantation Proceedings

      Volume: 53 Issue: 5 Pages: 1744-1750

    • DOI

      10.1016/j.transproceed.2021.04.012

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fabrication of functional rat pseudo‐islets after cryopreservation of pancreatic islets or dispersed islet cells2021

    • Author(s)
      Nakayama‐Iwatsuki Kenyu、Hirabayashi Masumi、Hochi Shinichi
    • Journal Title

      Journal of Tissue Engineering and Regenerative Medicine

      Volume: 15 Issue: 7 Pages: 686-696

    • DOI

      10.1002/term.3219

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats2021

    • Author(s)
      Toshihiro Kobayashi, Teppei Goto, Mami Oikawa, Makoto Sanbo, Fumika Yoshida, Reiko Terada, Naoko Niizeki, Naoyo Kajitani, Kanako Kazuki, Yasuhiro Kazuki, Shinichi Hochi, Hiromitsu Nakauchi, M. Azim Surani & Masumi Hirabayashi
    • Journal Title

      Nature Comminications

      Volume: 12 Issue: 1 Pages: 13281-10

    • DOI

      10.1038/s41467-021-21557-x

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transplantation of rat pancreatic islets vitrified-warmed on the nylon mesh device and the silk fibroin sponge disc2020

    • Author(s)
      Kenyu Nakayama-Iwatsuki, Takahiro Yamanaka, Jun Negishi, Junki Teshima, Yasushi Tamada, Masumi Hirabayashi & Shinichi Hochi
    • Journal Title

      Islets

      Volume: 12 Issue: 6 Pages: 145-155

    • DOI

      10.1080/19382014.2020.1849928

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] 近交系Brown-Norwayラットの精巣内精子による顕微授精 (TESE-ICSI)2023

    • Author(s)
      井出 美涼, 保地 眞一, 篠原 隆司 & 平林 真澄
    • Organizer
      第116回日本繁殖生物学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] MESA-ICSI/TESE-ICSIに用いるラット精子の凍結保存2023

    • Author(s)
      井出 美涼, 平林 真澄 & 保地 眞一
    • Organizer
      Cryopreservation Conference 2023
    • Related Report
      2023 Annual Research Report
  • [Presentation] ガラス化・加温ウシ成熟未受精卵へICSIしたときの胚盤胞発生率はIVFの場合と異なり新鮮対照に劣らない2022

    • Author(s)
      井出 美涼, 上野 清, 平林 真澄 & 保地 眞一
    • Organizer
      第115回日本繁殖生物学会
    • Related Report
      2022 Research-status Report
  • [Presentation] ガラス化ラット膵島の腎被膜下移植におけるシルクフィブロインスポンジの多目的連続使用2021

    • Author(s)
      藤本 空, 玉田 靖 & 保地 眞一
    • Organizer
      Cryopreservation Conference 2021
    • Related Report
      2021 Research-status Report
  • [Presentation] セミノリピド欠損マウスにおける雌性不妊および胆嚢拡張症解析2021

    • Author(s)
      中野 愛里, 上村 麻実, 金井 克晃, 藤原 祥高, 岩森 督子, 藤本 空, 根岸 淳, 保地 眞一 & 高島 誠司
    • Organizer
      第44回日本分子生物学会
    • Related Report
      2021 Research-status Report
  • [Presentation] シルクフィブロインディスクをデバイスとしてSSV法によってガラス化保存したラット膵島の腎被膜下移植2020

    • Author(s)
      中山(岩月) 研祐, 山中 貴寛, 根岸 淳, 手島 淳輝, 玉田 靖, 平林 真澄 & 保地 眞一
    • Organizer
      第67回日本実験動物学会
    • Related Report
      2020 Research-status Report
  • [Book] Silk Fibroin: Advances in Applications and Research2023

    • Author(s)
      S. Hochi, Y. Tamada
    • Total Pages
      332
    • Publisher
      Nova Science Publishers
    • ISBN
      9798886974027
    • Related Report
      2023 Annual Research Report

URL: 

Published: 2020-04-28   Modified: 2025-01-30  

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