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Elucidation of the species, pathogenicity and distribution of new Nosema pathogen of nosemosis in honeybee

Research Project

Project/Area Number 20K06426
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 42020:Veterinary medical science-related
Research InstitutionIwate University

Principal Investigator

Itagaki Tadashi  岩手大学, 農学部, 嘱託教授 (80203074)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsNosema ceranae / Nosema sp. / ニホンミツバチ / セイヨウミツバチ / ノゼマ病 / ミツバチ / 東北地方 / Nosema species / Apis cerana japonica / Apis millifera / DNA / Japan / 分布 / 種
Outline of Research at the Start

ミツバチのノゼマ病の原因種としてノゼマ科のNosema apis、N. ceranae、N. neumaniの3種が知られているが、東北地方のミツバチ類から、これら3種とは別種と考えられるNosema sp.が世界で初めて発見された。そこで本研究では、① Nosema sp.の分子学的および形態学的な同定、② Nosema sp.のミツバチ類に対する病原性の解明、③Nosema sp.特異的PCR法の新規開発、④日本国内のミツバチ類におけるNosema sp.感染状況の解明を目的とする。以上を明らかにすることでNosema sp.がノゼマ病の新たな病原体であるかを評価する。

Outline of Final Research Achievements

Honeybee nosemosis was designated in 1997 as a Monitored Infectious Disease by the Act on Domestic Animal Infectious Diseases Control published by the Minister of Agriculture, Forestry and Fisheries of Japan, and is a very crucial disease for Veterinary Medicine. However, the elucidation of the disease remains insufficient. This study clarified that the pathogen is not Nosema apis but Nosema ceranae and Nosema sp. in Tohoku district. The elucidation is very important for the disease prevention.

Academic Significance and Societal Importance of the Research Achievements

家畜伝染病予防法に指定されているミツバチ・ノゼマ病は獣医学的に重要な疾病であるにもかかわらず、同じ家畜である牛や豚などの家畜動物の感染症に比べて研究・解明は進んでいない。本研究により東北地方におけるノゼマ病の病原体が、指定されているNosema apisではなく、N. ceranaeおよびNosema sp.であることを明らかにしたことは本病の防遏対策を講じる上で極めて重要であり、学術的意義や社会的意義が大いにあると考えられる。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (6 results)

All 2022 2021 2020

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results)

  • [Journal Article] The causative agents of fascioliasis in animals and humans: Parthenogenetic Fasciola in Asia and other regions2022

    • Author(s)
      Tadashi Itagaki, Kei Hayashi, Yuma Ohari
    • Journal Title

      Infection, Genetics and Evolution

      Volume: - Pages: 105248-105248

    • DOI

      10.1016/j.meegid.2022.105248

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prevalence of Nosema species infections in Apis cerana japonica and Apis mellifera honeybees in the Tohoku region of Japan2021

    • Author(s)
      Shunsuke Takashima, Yuma Ohari, Tadashi Itagaki
    • Journal Title

      Parasitology International

      Volume: 83 Pages: 102361-102361

    • DOI

      10.1016/j.parint.2021.102361

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of a multiplex PCR method for discriminating between Heterakis gallinarum, H. beramporia, and H. indica parasites of poultry2021

    • Author(s)
      Peru Gopal Biswas, Yuma Oharia, Uday Kumar Mohanta, Tadashi Itagaki
    • Journal Title

      Veterinary Parasitology

      Volume: 295 Pages: 109463-109463

    • DOI

      10.1016/j.vetpar.2021.109463

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of <i>Ascaridia galli</i> from Bangladesh and development of a PCR method for distinguishing <i>A. galli</i> from <i>Heterakis</i> spp.2021

    • Author(s)
      Peru Gopal Biswas, Yuma Oharia, Uday Kumar Mohanta, Tadashi Itagaki
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 83 Issue: 4 Pages: 666-670

    • DOI

      10.1292/jvms.20-0728

    • NAID

      130008023261

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of conventional multiplex PCR method for discrimination between <i>Dispharynx nasuta</i> and <i>Cheilospirura hamulosa</i> (Nematoda: Acuariidae) parasitizing poultry2021

    • Author(s)
      Peru Gopal Biswas, Yuma Oharia, Uday Kumar Mohanta, Tadashi Itagaki
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 83 Issue: 2 Pages: 226-229

    • DOI

      10.1292/jvms.20-0664

    • NAID

      130007990730

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] The prevalence and molecular characterization of Acarapis woodi and Varroa destructor mites in honeybees in the Tohoku region of Japan2020

    • Author(s)
      Shunsuke Takashima, Yuma Ohari, Tadashi Itagaki
    • Journal Title

      Parasitology International

      Volume: 75 Pages: 1-4

    • DOI

      10.1016/j.parint.2020.102052

    • Related Report
      2020 Research-status Report
    • Peer Reviewed

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Published: 2020-04-28   Modified: 2025-01-30  

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