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Ribosome quality control via intron-containing tRNA (ic-tRNA) in budding yeast

Research Project

Project/Area Number 20K06491
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 43010:Molecular biology-related
Research InstitutionUniversity of Hyogo

Principal Investigator

HAYASHI Sachiko  兵庫県立大学, 理学研究科, 特任助教 (40791869)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordstRNA / イントロン / リボソーム / 翻訳 / 酵母
Outline of Research at the Start

イントロンを含む前駆体tRNA(Intron-containing tRNA; 以後、Ic-tRNA)は古細菌から真核生物に広く存在し、恒常的に発現している。しかし、その機能的役割はよく解っていない。tRNAイントロン欠失株を用いた表現型解析により、tRNAイントロンの有無は合成されるtRNA自身の機能性だけでなく、リボソーム機能にも影響を与え得ることが見出された。本研究では、tRNAイントロン欠失株で産生されるtRNAやリボソームの機能面への影響、それに伴うリボソーム関連品質管理機構への影響を検討し、Ic-tRNAが支える翻訳周りを中心とした細胞内環境の維持・制御機構の解明を目指す。

Outline of Final Research Achievements

The biological significance of introns in tRNA genes is still obscure. This study analyzed the impact of tRNA intron-deletion mutants in budding yeast on the general ribosomal quality control pathway. The deletion of introns from tRNA genes, particularly those that charge leucine (Leu) and have the anticodon CAA, suppressed ribosome stalling, which usually activates the ribosome-associated quality control (RQC) mechanism. This suppression led to an increase in aberrant translation. Furthermore, some of the translation products resulting from this aberrant translation formed aggregates in the cytoplasm, losing their functionality.

Academic Significance and Societal Importance of the Research Achievements

従来、翻訳異常や異常タンパク質の産生は、リボソームによる翻訳速度の低下やmRNA上の異常なコドンの読み枠による影響、tRNAアミノアシル化状態や分子シャペロン等の翻訳補助因子の量的・質的異常に主眼が置かれてきた。本研究は、ゲノム上のtRNAイントロンの有無という、これまで全く予想されて来なかった新たな局面からの異常が、一見、細胞内での全般的な翻訳を亢進させつつも、結果的には異常なタンパク質を産生、凝集体の集積を促すという、全く新しいプロテオスタシス異常のあり方を明らかにした。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (20 results)

All 2023 2022 2021 2020

All Journal Article (5 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (15 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Journal Article] Multiple layers of gene-expression regulatory mechanisms during fermentation and respiration2023

    • Author(s)
      Hayashi Sachiko
    • Journal Title

      New Advances in Saccharomyces (IntechOpen Book Series)

      Volume: - Pages: 1-29

    • DOI

      10.5772/intechopen.1003912

    • ISBN
      9780854661282, 9780854661275
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A non-canonical Puf3p-binding sequence regulates CAT5/COQ7 mRNA under both fermentable and respiratory conditions in budding yeast2023

    • Author(s)
      Hayashi Sachiko、Iwamoto Kazumi、Yoshihisa Tohru
    • Journal Title

      PLOS ONE

      Volume: 18 Issue: 12 Pages: e0295659-e0295659

    • DOI

      10.1371/journal.pone.0295659

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Six identical tRNATrpCCA genes express a similar amount of mature tRNATrpCCA but unequally contribute to yeast cell growth2022

    • Author(s)
      Sachiko Hayashi, Masaya Matsui, Ayano Ikeda, Tohru Yoshihisa
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 86 Issue: 10 Pages: 1398-1404

    • DOI

      10.1093/bbb/zbac134

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] 出芽酵母のイントロン含有tRNA(ic-tRNA)が支えるリボソーム関連品質管理2022

    • Author(s)
      林 紗千子
    • Journal Title

      月刊「細胞」特集「タンパク質翻訳制御の最前線」

      Volume: 54

    • Related Report
      2022 Research-status Report
  • [Journal Article] Puf3p facilitates fermentative mitochondrial functions via monosome-enriched nuclear-encoded mitochondrial mRNAs in budding yeast2022

    • Author(s)
      Sachiko Hayashi, Kazumi Iwamoto, Tohru Yoshihisa
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2022.04.10.487782

    • Related Report
      2021 Research-status Report
    • Open Access
  • [Presentation] Intricated connection of the tRNALeuCAA intron with cytosolic ribosomes and mitochondrial gene expression in budding yeast.2023

    • Author(s)
      Sachiko Hayashi, Hitomi Kato, Ryujiro Nakamura, Yuichi Shichino, Shintaro Iwasaki, and Tohru Yoshihisa
    • Organizer
      第24回日本RNA学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 出芽酵母のtRNALeuCAAイントロン欠失株で生産されるリボソームに関する解析2023

    • Author(s)
      中村龍二郎, 加藤瞳, 吉久徹, 林紗千子
    • Organizer
      第46回日本分子生物学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 出芽酵母のCAT5/COQ7 mRNAは非典型的結合配列により、呼吸と発酵の両方でPuf3pに制御される2023

    • Author(s)
      林紗千子, 岩元夏純, 吉久徹
    • Organizer
      第46回日本分子生物学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Pumilio homologue Puf3p coordinates CAT5/COQ7 expression non-canonical binding-sequence dependently2022

    • Author(s)
      Sachiko Hayashi, Kazumi Iwamoto, Tohru Yoshihisa
    • Organizer
      Cold Spring Harbor Asia, RNA Biology 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Regulation of tRNA repertoires in the budding yeast2022

    • Author(s)
      Nayuko Sasada, Momoka Irie, Akihisa Nagai, Moeka Taniwaki, Sachiko Hayashi, Tohru Yoshihisa
    • Organizer
      Cold Spring Harbor Asia, RNA Biology 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] 酵母の6 つの完全に相同なtRNATrp CCA 遺伝子 は、等しく成熟tRNATrp CCA を供給するものの、生育に遺伝子座固有の影響を持つ2022

    • Author(s)
      林紗千子, 松井将也, 池田彩乃, 吉久徹
    • Organizer
      第 45 回日本分子生物学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] Mysterious link with the absence or presence of tL(CAA) intron; cytosolic and mitochondrial translation possibly related with Rps7 paralog selectivity in budding yeast2022

    • Author(s)
      Sachiko Hayashi and Tohru Yoshihisa
    • Organizer
      第 45 回日本分子生物学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] Paralogue-specific regulation of RPS7/eS7 mRNAs via the 3’-UTR in budding yeast2022

    • Author(s)
      Sachiko Hayashi and Tohru Yoshihisa
    • Organizer
      第23 回 日本RNA 学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] Formation and maintenance of tRNA repertoires in Saccharomyces cerevisiae2022

    • Author(s)
      Nayuko Sasada, Momoka Irie, Moeka Taniwaki, Masaya Matsui, Ayano Ikeda, Sachiko Hayashi, Akihisa Nagai and Tohru Yoshihisa
    • Organizer
      第30 回Tokyo RNA Club
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] tRNALeuCAA modulates ribosome biogenesis and functions2021

    • Author(s)
      Sachiko Hayashi, Hitomi Kato, Tohru Yoshihisa
    • Organizer
      第22回日本RNA学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] S. cerevisiae tRNA-LeuCAA keeps up ribosome biogenesis2021

    • Author(s)
      Sachiko Hayashi, Hitomi Kato, Yuichi Shichino, Shintaro Iwasaki, Tohru Yoshihisa
    • Organizer
      EMBL Conference: Protein Synthesis and Translational Control
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Puf3p facilitates non-respiratory mitochondrial functions via monosome-enriched nuclear-encoded mitochondrial mRNAs in budding yeast2021

    • Author(s)
      Sachiko Hayashi, Kazumi Iwamoto, Tohru Yoshihisa
    • Organizer
      第44回日本分子生物学会年会(MBSJ2021)
    • Related Report
      2021 Research-status Report
  • [Presentation] Translational regulations for nuclear-encoded mitochondrial mRNAs identified in monosome2020

    • Author(s)
      Sachiko Hayashi, Kazumi Iwamoto, Tohru Yoshihisa
    • Organizer
      25th Annual Meeting of the RNA Society (RNA2020)
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] Puf3p regulates a subset of monosome-enriched mRNAs for mitochondrial proteins upon non-respiratory condition2020

    • Author(s)
      Sachiko Hayashi, Kazumi Iwamoto, Tohru Yoshihisa
    • Organizer
      第43回 日本分子生物学会年会(MBSJ2020 Online)
    • Related Report
      2020 Research-status Report
  • [Presentation] tRNA-LeuCAA keeps up ribosome biogenesis and functions2020

    • Author(s)
      Hitomi Kato, Tohru Yoshihisa, Sachiko Hayashi
    • Organizer
      第43回 日本分子生物学会年会(MBSJ2020 Online)
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2025-01-30  

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