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Relationship between structural symmetry and function of the torque-generating unit in bacterial flagellar motors

Research Project

Project/Area Number 20K06564
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 43030:Functional biochemistry-related
Research InstitutionHosei University

Principal Investigator

Sowa Yoshiyuki  法政大学, 生命科学部, 教授 (10519440)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsべん毛モーター / 回転計測 / 固定子 / べん毛 / 分子モーター
Outline of Research at the Start

べん毛モーターは,人工のモーターと非常に似た構造をしており,固定子と回転子から構成される.固定子内を透過するイオン流から得られるエネルギーがモーターの駆動力である.モーターが回転を生み出すためには,固定子がもつ2つのプロトン透過経路が必要か,それとも1つのプロトン透過経路で十分であるかという問いを検証し,化学-力学エネルギー共役について考察する.

Outline of Final Research Achievements

The bacterial flagellar motor is a reversible rotary nano-machine that rotates at high speed and runs with high efficiency. This study focused on the stator unit responsible for the chemical-mechanical energy conversion. We characterized the “hetero” stator unit by breaking its structural symmetry, the stator assembly depending on the load to the motor, the specificity of coupling ions on stator units, and newly developed fluorescent protein-fused stator units. The results obtained here are significant in that they will provide the basic technology for further analysis of the motor rotation.

Academic Significance and Societal Importance of the Research Achievements

細菌べん毛モーターは,人工モーターでは実現できないような高効率,高速回転を実現するわずか直径50 nmの分子機械である.このモーターの回転機構が解明されれば,人工的な小型モーターの開発にも貢献できると期待される.本研究では,エネルギー変換の中心を担う固定子ユニットについて研究をおこない,今後の研究の基盤的な技術となる手法を構築できた.

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (27 results)

All 2024 2023 2022 2021 2020 Other

All Int'l Joint Research (4 results) Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (18 results) (of which Invited: 2 results)

  • [Int'l Joint Research] UNSW(オーストラリア)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] UNSW(オーストラリア)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] UNSW(オーストラリア)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] UNSW(オーストラリア)

    • Related Report
      2020 Research-status Report
  • [Journal Article] Flagellar polymorphism-dependent bacterial swimming motility in a structured environment2023

    • Author(s)
      Yoshiaki Kinosita, Yoshiyuki Sowa
    • Journal Title

      Biophysics and Physicobiology

      Volume: 20 Issue: 2 Pages: n/a

    • DOI

      10.2142/biophysico.bppb-v20.0024

    • ISSN
      2189-4779
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The rapid evolution of flagellar ion selectivity in experimental populations of E. coli2022

    • Author(s)
      Ridone Pietro、Ishida Tsubasa、Lin Angela、Humphreys David T.、Giannoulatou Eleni、Sowa Yoshiyuki、Baker Matthew A. B.
    • Journal Title

      Science Advances

      Volume: 8 Issue: 47

    • DOI

      10.1126/sciadv.abq2492

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Novel Amiloride Derivatives That Inhibit Bacterial Motility across Multiple Strains and Stator Types2021

    • Author(s)
      Islam M. I.、Bae J. H.、Ishida T.、Ridone P.、Lin J.、Kelso M. J.、Sowa Y.、Buckley B. J.、Baker M. A. B.
    • Journal Title

      Journal of Bacteriology

      Volume: 203 Issue: 22

    • DOI

      10.1128/jb.00367-21

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Distinct chemotactic behavior in the original Escherichia coli K-12 depending on forward-and-backward swimming, not on run-tumble movements2020

    • Author(s)
      Kinosita Yoshiaki、Ishida Tsubasa、Yoshida Myu、Ito Rie、Morimoto Yusuke V.、Goto Kazuki、Berry Richard M.、Nishizaka Takayuki、Sowa Yoshiyuki
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 15887-15887

    • DOI

      10.1038/s41598-020-72429-1

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Coupling Ion Specificity of the Flagellar Stator Proteins MotA1/MotB1 of Paenibacillus sp. TCA202020

    • Author(s)
      Onoe Sakura、Yoshida Myu、Terahara Naoya、Sowa Yoshiyuki
    • Journal Title

      Biomolecules

      Volume: 10 Issue: 7 Pages: 1078-1078

    • DOI

      10.3390/biom10071078

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 大腸菌センサーキナーゼBaeSから走化性制御因子CheYへのクロストーク2024

    • Author(s)
      武井 陸,井芹 友香,大森 楓河,田島 寛隆,石黒 亮,日高 直樹,吉村 美歩,曽和 義幸,西川 正俊,山本 兼由,川岸 郁朗
    • Organizer
      2023年度べん毛研究交流会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 細菌べん毛モーター制御因子FliLの固定子との相互作用の解析2024

    • Author(s)
      熊谷 桃花,石田 翼,日髙 直樹,曽和 義幸
    • Organizer
      2023年度べん毛研究交流会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Stator dynamics of chimeric Na+-driven E. coli flagellar motor observed with fluorescent microscopy2023

    • Author(s)
      Tomoya Shoji, Naoki Hidaka, Yong-Suk Che, Yoshiyuki Sowa
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] The L118P mutation in the stator protein MotB affects motor adaptation to load in the bacterial flagellar motor2023

    • Author(s)
      Shuo Peng Wang, Tsubasa Ishida, Naoki Hidaka, Shoichi Toyabe, Seiji Kojima, Yoshiyuki Sowa
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Heterotrimer formation of MdtB and MdtC, inner membrane transporters of the RND-type xenobiotic efflux complex2023

    • Author(s)
      Yuto Nakamura, Kenichiro Kashihara, Hirotaka Tajima, Yoshiyuki Sowa & Ikuro Kawagishi
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Development of an optical method to control bacterial motility using light-driven proton pumping rhodopsins2023

    • Author(s)
      Kotaro Nakanishi, Keiichi Kojima, Yoshiyuki Sowa, Yuki Sudo
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Analysis of the interaction interface between the rotor and stator of the bacterial flagellar motor at the amino acid residue level2022

    • Author(s)
      Nakaya J, Kumazaki Y, Ishida T, Yoshida M, Ito R, Sowa Y
    • Organizer
      第60回日本生物物理学会
    • Related Report
      2022 Research-status Report
  • [Presentation] Identification of the indole-sensing region of the sensor kinase BaeS2022

    • Author(s)
      Tajima H, Kashihara K, Yamamoto K, Sowa Y, Kawagishi I
    • Organizer
      第60回日本生物物理学会
    • Related Report
      2022 Research-status Report
  • [Presentation] Role of divalent metal cations in ligand recognition by the Salmonella citrate chemoreceptor Tcp2022

    • Author(s)
      Omori F, Matsuda M, Imada K, Tajima H, Sowa Y, Kawagishi I
    • Organizer
      第60回日本生物物理学会
    • Related Report
      2022 Research-status Report
  • [Presentation] FliLが制御するべん毛モーターの負荷感受性2022

    • Author(s)
      石田翼,吉多美祐,南野徹,曽和義幸
    • Organizer
      2021年度べん毛研究交流会
    • Related Report
      2021 Research-status Report
  • [Presentation] 放線菌Actinoplanes missouriensisの極べん毛モーター回転計測2022

    • Author(s)
      高井亮太郎,手塚武揚,石田翼,大西康夫,曽和義幸
    • Organizer
      2021年度べん毛研究交流会
    • Related Report
      2021 Research-status Report
  • [Presentation] 光架橋法と蛍光観察によるべん毛モーターの固定子ダイナミクスの解析2022

    • Author(s)
      竜野真理衣,熊崎優美,石田翼,曽和義幸
    • Organizer
      2021年度べん毛研究交流会
    • Related Report
      2021 Research-status Report
  • [Presentation] 大腸菌FliLは低負荷領域でべん毛モーターの回転を支援する2021

    • Author(s)
      石田翼,吉多美祐,南野徹,曽和義幸
    • Organizer
      第1回 発動分子科学研究会
    • Related Report
      2021 Research-status Report
  • [Presentation] 大腸菌FliLは低負荷条件下でべん毛モーターの回転速度を調節する2021

    • Author(s)
      石田翼,吉多美祐,南野徹,曽和義幸
    • Organizer
      第17回21世紀大腸菌研究会
    • Related Report
      2021 Research-status Report
  • [Presentation] 大腸菌べん毛モーターの出力制御因子の解析2020

    • Author(s)
      曽和義幸
    • Organizer
      第10回分子モーター討論会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Bacterial flagellar rotation at low load2020

    • Author(s)
      Y. Sowa, T. Ishida
    • Organizer
      第58回日本生物物理学会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] FliL assists flagellar motor rotation in Escherichia coli under low load condition2020

    • Author(s)
      T. Ishida, M. Yoshida, T. Minamino, Y. Sowa
    • Organizer
      第58回日本生物物理学会
    • Related Report
      2020 Research-status Report
  • [Presentation] Low cooperativity of flagellar motor switching in Vibrio cholerae the bacterium of a single polar flagellum2020

    • Author(s)
      H. Tajima, M. Nishikawa, Y. Miura, Y. Sowa, I. Kawagishi
    • Organizer
      第58回日本生物物理学会
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2025-01-30  

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