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Regulation of tissue-specific microtubule arrays

Research Project

Project/Area Number 20K06616
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44010:Cell biology-related
Research InstitutionTohoku University

Principal Investigator

Haruta Nami  東北大学, 生命科学研究科, 助教 (70381671)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords微小管 / gamma-tubulin complex / C.elegans / 微小管形成中心 / γ-チューブリン複合体 / 微小管動態 / Caenorhabditis elegans / γ-チューブリン / 生殖腺
Outline of Research at the Start

動物の器官をつくる組織の細胞形態は多様であるが、細胞形態を決定づける因子の一つが細胞骨格の微小管である。
本研究では、線虫C. elegansの生殖線内の生殖細胞系列における微小管動態とその制御メカニズムを調べる。γ-チューブリン複合体をはじめとする微小管制御タンパク質を時期および組織特異的にノックダウンし、ライブイメージング観察を行うことで、γ-チューブリン複合体の生殖細胞系列の膜への局在化機構を解明し、組織特異的な微小管ネットワークの構築と制御機構の理解を目指す。

Outline of Final Research Achievements

Microtubule (MT) plays various roles in cellular functions such as cytokinesis, establishment of cell polarity and morphogenesis. The γ-tubulin complex (γTuC) is a major nucleation factor of MTs. We identified GTAP-1 and GTAP-2 as a novel component of C. elegans γ-tubulin complex (γTuC) for the efficient recruitment of γTuC to the centrosomes. To characterize the role of GTAP-1 and GTAP-2 in germline, we constructed the strain expressing fluorescent-tagged marker protein and observed with wild-type, gtap-1 and gtap-2 background. The gtap-1 mutant showed abnormal germline morphology and abnormal laid eggs in age-dependent manner. Even though both GTAP-1 and GTAP-2 involved in the efficient recruitment of the γ-tubulin onto plasma membrane of germline, only GTAP-1 might be involved the regulation of γTuC. These results indicate that GTAP-1 and GTAP-2 has different roles in the non-centrosomal MTOC in the specific tissues.

Academic Significance and Societal Importance of the Research Achievements

本研究から、γ-チューブリン複合体(γTuC)構成因子であるGTAP-1とGTAP-2の生殖腺膜上の微小管形成中心における役割が明確に異なることが分かった。さらにγ-チューブリンの局在量と微小管の表現型とが一致しないことから、局在化とγTuCの制御機構を分けられることを強く示唆する。
この結果は、線虫のみならず、他の動物種における組織特異的なγTuCによる微小管形成能の制御機構と組織形成に関与する微小管ネットワーク構造の構築原理の理解する上で重要な知見となる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (17 results)

All 2023 2022 2021 2020 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (12 results) (of which Int'l Joint Research: 7 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Centrosome maturation requires phosphorylation-mediated sequential domain interactions of SPD-5.2022

    • Author(s)
      Nakajo, M., Kano, H., Tsuyama, K., *Haruta, N. and *Sugimoto, A.
    • Journal Title

      Journal of Cell Sciences

      Volume: 135 Issue: 8

    • DOI

      10.1242/jcs.259025

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ransgenesis of the gonochoristic nematode Caenorhabditis inopinata by microparticle bombardment with hygromycin B selection.2022

    • Author(s)
      Oomura, S. Tsuyama, K., Haruta, N, and *Sugimoto A
    • Journal Title

      Micropublication

      Volume: -

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans2022

    • Author(s)
      Negishi T, Kitagawa S, Horii N, Tanaka Y, Haruta N, Sugimoto A, Sawa H, Hayashi KI, Harata M, Kanemaki MT.
    • Journal Title

      Genetics

      Volume: 220 Issue: 2

    • DOI

      10.1093/genetics/iyab218

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression Patterns and Levels of All Tubulin Isotypes Analyzed in GFP Knock-In <i>C. elegans</i> Strains2021

    • Author(s)
      Nishida Kei、Tsuchiya Kenta、Obinata Hiroyuki、Onodera Shizuka、Honda Yu、Lai Yen-Cheng、Haruta Nami、Sugimoto Asako
    • Journal Title

      Cell Structure and Function

      Volume: 46 Issue: 1 Pages: 51-64

    • DOI

      10.1247/csf.21022

    • NAID

      130008059056

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] The tissue-specific role of the unconventional components of the γ-tubulin complex in C. elegans2023

    • Author(s)
      N. Haruta, and A. Sugimoto
    • Organizer
      Naito conference 2023
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] he germline-specific role of the unconventional components of the γ-tubulin complex in C. elegans2023

    • Author(s)
      N. Haruta, and A. Sugimoto
    • Organizer
      Cold spring harbor Asia 2023 “Cilia and Centrosomes”
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 紡錘体動態および非対称分裂の進化細胞生物学的解析2022

    • Author(s)
      春田奈美、生井聡史、佐々木大地、杉本亜砂子
    • Organizer
      細胞分裂研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Distinct properties of broadly-expressed and tissue-specific tubulin isotypes examined by ectopic and heterologous expression2021

    • Author(s)
      Kei Nishida, Kenta Tsuchiya, Yu Honda, Hiroyuki Obinata, Shizuka Onodera, Nami Haruta, Masanori Ikeda, Kozo Tanaka, Asako Sugimoto
    • Organizer
      23rd international C. elegans conference
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Comparative analysis of cellular dynamics of C. inopinata and C. elegans zygotes2021

    • Author(s)
      Shun Oomura, Shuichi Onami, Koji Kyoda, Nami Haruta, Asako Sugimoto
    • Organizer
      23rd international C. elegans conference
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Significant differences in the sex determination pathways between C. inopinata and C. elegans2021

    • Author(s)
      Ryuhei Hatanaka, Yuki Hoshi, Nami Haruta, Taisei Kikuchi and Asako Sugimoto
    • Organizer
      23rd international C. elegans conference
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] 線虫C. elegansを用いた感覚神経特異的チューブリンアイソタイプ群の特 性解析2021

    • Author(s)
      西田 桂、土屋 賢汰、春田 奈美、池田 真教、田中 耕三、杉本 亜砂子
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] 線虫Pristionchus pacificusにおける生殖顆粒構成因子の同定と機能 解析2021

    • Author(s)
      野呂美波、パン・ウィキュ、生井聡史、春田奈美、杉本亜砂子
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] 中心体と組織特異的な微小管形成中心2020

    • Author(s)
      春田奈美、中條桃江、杉本亜砂子
    • Organizer
      日本分子生物学会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 線虫C.elegansにおける中心体周辺物質の足場形成機構の解析2020

    • Author(s)
      中條桃江、狩野ひかる、春田奈美、杉本亜砂子
    • Organizer
      日本分子生物学会
    • Related Report
      2020 Research-status Report
  • [Presentation] Comprehensive expression analysis of tubulin isotypes using GFP-knock-in strains in C.elegans2020

    • Author(s)
      Kei Nishida, Kenta Tsuchiya, Yu Honda, Hiroyuki Obinata, Shizuka Onodera, Nami Haruta, Masanori Ikeda, Kozo Tanaka, Asako Sugimoto
    • Organizer
      EMBO/EMBL Symposia Microtubules
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] Early embryogenesis of Caenorhabditis inopinata, the closest species of Caenorhabditis elegans2020

    • Author(s)
      Shun Oomura, Yuki Matsumura, Nami Haruta and Asako Sugimoto
    • Organizer
      Ecology, Evolution and Genomics of C. elegans and Other Nematodes
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Remarks] 発生ダイナミクス分野杉本研究室

    • URL

      https://devdyn.wixsite.com/sugimoto-lab

    • Related Report
      2022 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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