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Diversity of nuclear pore structure in the binucleate ciliate Tetrahymena

Research Project

Project/Area Number 20K06632
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44010:Cell biology-related
Research InstitutionNihon University

Principal Investigator

IWAMOTO Masaaki  日本大学, 文理学部, 教授 (80450683)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords核膜孔複合体 / ヌクレオポリン / 免疫電子顕微鏡法 / 核分化 / テトラヒメナ / 繊毛虫 / Nup107-160複合体
Outline of Research at the Start

核膜孔複合体の構造は、単細胞生物から高等動物に至るまでよく保存されたものであると考えられているが、単細胞生物のテトラヒメナが持つ二種類の核の核膜孔複合体は、共通の核膜孔タンパク質で構築されているにも関わらず構造が異なっていると予想される。本研究では、テトラヒメナの核膜孔タンパク質各種が核膜孔複合体内部のどこに配置しているのかを免疫電子顕微鏡法によって解析し、テトラヒメナの核膜孔複合体がどのように構築されており、それらが大核と小核の間で、あるいは他の生物種の核膜孔複合体との間でどのように異なっているのかを明らかにする。

Outline of Final Research Achievements

The ciliate Tetrahymena is a binucleate organism that has two types of nuclei with different functions, the macronucleus (MAC) and the micronucleus (MIC), within the same cell. It was known that the nuclear pore complexes (NPCs) of the MAC and MIC are composed of partly different but mostly common nuclear pore proteins. However, it was unclear whether the core scaffolds of the NPCs formed by these common components were the same shape or different between MAC and MIC. In this study, the arrangement of the common nuclear pore proteins within the NPC was examined by immunoelectron microscopy. It was found that the same set of nuclear pore proteins form core scaffolds with largely different shapes in the NPCs of MAC and MIC.

Academic Significance and Societal Importance of the Research Achievements

核膜孔複合体に関するこれまでの研究の多くは、オピストコンタに属する出芽酵母や哺乳類細胞を用いて行われてきたことから、核膜孔複合体の構造と機能は単細胞生物から高等動物まで広く保存されたものと認識されている。しかしながら、アルベオラータに属するテトラヒメナの大核の核膜孔複合体は、まったく新奇の構造を呈するものだった。この知見は、核膜孔複合体は考えられていた以上にバリエーションに富んだ構造体であることを示唆しており、テトラヒメナの核膜孔複合体の多様な構造と、大核および小核に特有の核機能との関連性を調べることによって、今後、核膜孔複合体の未知の機能が明らかになっていくものと期待される。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (10 results)

All 2023 2022 2021 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (2 results)

  • [Journal Article] Live CLEM imaging of Tetrahymena to analyze the dynamic behavior of the nuclear pore complex2022

    • Author(s)
      Tokuko Haraguchi, Hiroko Osakada, Masaaki Iwamoto
    • Journal Title

      Methods in Molecular Biology

      Volume: 2502 Pages: 473-492

    • DOI

      10.1007/978-1-0716-2337-4_30

    • ISBN
      9781071623367, 9781071623374
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Live CLEM imaging of Tetrahymena to analyze the dynamic behavior of the nuclear pore complex2021

    • Author(s)
      Tokuko Haraguchi, Hiroko Osakada, Masaaki Iwamoto
    • Journal Title

      Methods in Molecular Biology

      Volume: -

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] Nuclear pore complexes of dimorphic nuclei: Their distinct structures related to nuclear differentiation2023

    • Author(s)
      Masaaki Iwamoto
    • Organizer
      2023 Ciliate Molecular Biology Meeting
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The nuclear pore complexes of ciliates’ dimorphic nuclei: different structures composed of the same components2022

    • Author(s)
      岩本政明,小坂田裕子,平岡泰,原口徳子
    • Organizer
      日本細胞生物学会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 繊毛虫テトラヒメナの配偶核形成におけるSemi1とSemi2タンパク質の協調作用2022

    • Author(s)
      明松隆彦, 福田康弘, 岩本政明
    • Organizer
      日本原生生物学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Basal bodies in the anterior region of the Tetrahymena cell are binding sites for nucleoporin MicNup98A2021

    • Author(s)
      Takaharu Yamamoto, Noriko Fukuta, Masaaki Iwamoto, Atsushi Matsuda
    • Organizer
      2021 Ciliate Molecular Biology Meeting
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] 細胞の前端部に局在する繊毛虫テトラヒメナのヌクレオポリンMicNup98A2021

    • Author(s)
      山本孝治, 福田紀子, 近重裕次, 岩本政明, 松田厚志
    • Organizer
      日本原生生物学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 細胞の前端部に局在する繊毛虫テトラヒメナのタンパク質2021

    • Author(s)
      山本孝治, 福田紀子, 岩本政明, 松田厚志
    • Organizer
      日本分子生物学会
    • Related Report
      2021 Research-status Report
  • [Remarks] 日本大学文理学部生命科学科原生生物学研究室

    • URL

      https://dept.chs.nihon-u.ac.jp/biosciences/staff/iwamoto/

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
  • [Remarks] 岩本 政明 研究室 研究紹介

    • URL

      http://biosci.chs.nihon-u.ac.jp/teacher/iwamoto/research-introduction#02

    • Related Report
      2020 Research-status Report

URL: 

Published: 2020-04-28   Modified: 2024-01-30  

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