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Functional analyses on WOX trancription factors and peptide hormones in tissue wound repair

Research Project

Project/Area Number 20K06712
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionNara Institute of Science and Technology (2022)
Niigata University (2020-2021)

Principal Investigator

Ikeuchi Momoko  奈良先端科学技術大学院大学, 先端科学技術研究科, 特任准教授 (00633570)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords組織修復 / カルス / ホメオボックス / 器官再生 / 組織治癒 / 接ぎ木 / 転写制御 / 傷害応答 / 傷シグナル / 細胞分裂 / 細胞伸長
Outline of Research at the Start

植物は器官が傷つくと、活発に細胞分裂と細胞伸長を行って細胞塊であるカルスを形成して速やかに傷口を塞ぎ、水分の損失や病原菌の侵入を防ぐ。私はこれまでに、カルス形成の制御において重要な役割を果たす転写因子を発見している。さらに、この転写因子が、ペプチド性のホルモンなどの細胞間シグナルに関わる遺伝子や、細胞壁のつながりを制御する酵素などの遺伝子発現を調節していることが見えてきた。そこで本研究では、こうした因子群がカルス形成において果たす役割を明らかにする。

Outline of Final Research Achievements

Plants can repair damaged tissues via callus formation. We recently identified WOX13 as a key regulator of tissue repair processes. We found that the expression of WOX13 is induced by damage signal. WOX13 in turn induces genes involved in cell wall modification. We also uncovered that auxin-induced expression of WOX13 is responsible for the differential callus formation activity between two opposing ends of the cut organs. Taken together, our studies revealed that organ cutting provokes both local damage signal and the mislocalization of long distance signal, together strongly activate WOX13 to enable efficient tissue repair.

Academic Significance and Societal Importance of the Research Achievements

植物の組織修復能力を利用して、苗の接ぎ木が農業や園芸分野で幅広く用いられている。本研究によって組織修復および切断された機関の再接着において重要な制御因子を同定しその機能解析を進められたことにより、接ぎ木メカニズムの一端を明らかすることができた。将来的には、モデル植物を用いた本研究成果を農作物へ応用することによって、接ぎ木技術の開発につなげられることが期待できる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (13 results)

All 2022 2021 2020 Other

All Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 7 results,  Open Access: 6 results) Presentation (4 results) (of which Invited: 2 results) Remarks (1 results)

  • [Journal Article] Auxin-Induced WUSCHEL-RELATED HOMEOBOX13 Mediates Asymmetric Activity of Callus Formation upon Cutting2022

    • Author(s)
      Tanaka Hayato、Hashimoto Naoki、Kawai Satomi、Yumoto Emi、Shibata Kyomi、Tameshige Toshiaki、Yamamoto Yuma、Sugimoto Keiko、Asahina Masashi、Ikeuchi Momoko
    • Journal Title

      Plant and Cell Physiology

      Volume: 64 Issue: 3 Pages: 305-316

    • DOI

      10.1093/pcp/pcac146

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Warm Temperature Promotes Shoot Regeneration in <i>Arabidopsis thaliana</i>2022

    • Author(s)
      Lambolez Alice、Kawamura Ayako、Takahashi Tatsuya、Rymen Bart、Iwase Akira、Favero David S、Ikeuchi Momoko、Suzuki Takamasa、Cortijo Sandra、Jaeger Katja E、Wigge Philip A、Sugimoto Keiko
    • Journal Title

      Plant and Cell Physiology

      Volume: ー Issue: 5 Pages: 618-634

    • DOI

      10.1093/pcp/pcac017

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Plant stem cell research is uncovering the secrets of longevity and persistent growth2021

    • Author(s)
      Umeda Masaaki、Ikeuchi Momoko、Ishikawa Masaki、Ito Toshiro、Nishihama Ryuichi、Kyozuka Junko、Torii Keiko U.、Satake Akiko、Goshima Gohta、Sakakibara Hitoshi
    • Journal Title

      The Plant Journal

      Volume: Online ahead of print Issue: 2 Pages: 1-10

    • DOI

      10.1111/tpj.15184

    • Related Report
      2021 Research-status Report 2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Wound-inducible WUSCHEL-RELATED HOMEOBOX 13 is required for callus growth and organ reconnection2021

    • Author(s)
      Ikeuchi Momoko, Iwase Akira, Ito Tasuku, Tanaka Hayato, Favero David S, Kawamura Ayako, Sakamoto Shingo, Wakazaki Mayumi, Tameshige Toshiaki, Fujii Haruki, Hashimoto Naoki, Suzuki Takamasa, Hotta Kazuhiro, Toyooka Kiminori, Mitsuda Nobutaka, Sugimoto Keiko
    • Journal Title

      Plant Physiology

      Volume: 188 Issue: 1 Pages: 425-441

    • DOI

      10.1093/plphys/kiab510

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] WIND transcription factors orchestrate wound-induced callus formation, vascular reconnection and defense response in Arabidopsis2021

    • Author(s)
      Iwase A, Kondo Y, Laohavisit A, Takebayashi A, Ikeuchi M, Matsuoka K, Asahina M, Mitsuda N, Shirasu K, Fukuda H, Sugimoto K
    • Journal Title

      New Phytologist

      Volume: 232 Issue: 2 Pages: 734-752

    • DOI

      10.1111/nph.17594

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The SUMO E3 ligase SIZ1 negatively regulates shoot regeneration2020

    • Author(s)
      Duncan Coleman, Ayako Kawamura, Momoko Ikeuchi, David Seth Favero, Alice Lambolez, Bart Rymen, Akira Iwase, Takamasa Suzuki, Keiko Sugimoto
    • Journal Title

      Plant Physiology

      Volume: 184 Issue: 1 Pages: 330-344

    • DOI

      10.1104/pp.20.00626

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] How do plants transduce wound signals to induce tissue repair and organ regeneration?2020

    • Author(s)
      Ikeuchi Momoko、Rymen Bart、Sugimoto Keiko
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 57 Pages: 72-77

    • DOI

      10.1016/j.pbi.2020.06.007

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 傷口からの再生ー植物の細胞リプログラミング2020

    • Author(s)
      池内桃子、杉本慶子
    • Journal Title

      生物の科学 遺伝

      Volume: 5 Pages: 568-573

    • Related Report
      2020 Research-status Report
  • [Presentation] 器官の再接着を可能にするカルス細胞の出会い2022

    • Author(s)
      池内桃子
    • Organizer
      日本植物学会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] How do plants re-construct a new life upon injury?2021

    • Author(s)
      Momoko Ikeuchi
    • Organizer
      日本分子生物学会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] シュート再生系におけるWUS とWOX13 の発現動態の解析2021

    • Author(s)
      小倉菜緒、池内桃子
    • Organizer
      日本植物学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 器官再生における細胞リプログラミングの制御機構2020

    • Author(s)
      池内桃子
    • Organizer
      日本植物学会
    • Related Report
      2020 Research-status Report
  • [Remarks] 植物が切断されても、傷口を修復してつなげる仕組みを解明

    • URL

      http://www.naist.jp/pressrelease/2022/10/009356.html

    • Related Report
      2022 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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