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Intrasurvival methanism of Escherichia albertii in Acanthamoeba

Research Project

Project/Area Number 20K07498
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 49050:Bacteriology-related
Research InstitutionKagoshima University

Principal Investigator

Ooka Tadasuke  鹿児島大学, 医歯学域医学系, 准教授 (50363594)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsEscherichia albertii / アメーバ内増殖機構 / 菌濃縮法 / タイピング / 病原性 / アメーバ内増殖 / IgG結合タンパク質 / 鞭毛抗原多様性 / 病原機構 / 分離培養法 / 環境動態
Outline of Research at the Start

近年、新興下痢症起因菌Escherichia albertiiによる感染症・食中毒が多数報告されているが、本菌の性状に不明な点が多く、感染経路の同定に至らないケースも多く、また保菌宿主および環境動態もを明らかになっていない。本研究では、「自然の水環境中においてアメーバを宿主として安定的に生存できる」可能性に焦点を当て、①その増殖機構の解明、②検査応用に向けたアメーバを用いた分離培養法の確立、③本分離培養法による水環境での分布および分離株と臨床由来株との遺伝的性状の異同の解明を行う。これらの研究から、感染経路の同定を容易にし、本菌による水環境からの感染リスクに関する理解を大きく前進させる。

Outline of Final Research Achievements

Escherichia albertii is a recently recognized human enteropathogen. In this study, we performed the functional analysis to identify the mechanism of intracellular-survival in Acanthamoeba castellanii of this species and also the virulence mechanism in mammalian cells. In addition, we tried to develop effective methods to identify the source of infection and to perform the survey of prevalence of this species in the environment.
The results revealed that 1)EACBF1370 protein is important for intracellular survival in A. castellanii, 2)we identified the E. albertii-specific surface factor and developed the immunomagnetic beads-based concentration and enrichment method for this species, 3)the fliC genes of E. albertii were grouped into four clearly distinguishable types designated E. albertii H-genotypes (EAHg1-EAHg4).

Academic Significance and Societal Importance of the Research Achievements

本研究では、集団感染の起因菌となっている新興下痢症起因菌E. albertii (EA)について、ゲノム情報および先行研究の知見を基に解析を進め、本菌の環境(特に水環境)中での生態に関する情報を得ることができ、本菌の感染経路を推定する上での有用な知見を得ることが出来た。また、本菌による感染事例では、感染源に存在する菌数が少ないためかその同定に至る例が少ないことが問題であるが、本研究において、EAの表層分子を利用した菌濃縮法および菌タイピング法を開発し、その有用性も明らかに出来たことで、今後の迅速な感染源同定などに繋がることが期待される。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (10 results)

All 2022 2021 2020 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (4 results) (of which Invited: 1 results)

  • [Int'l Joint Research] Federal University of Sao Paulo/Universidade Estadual Paulista/Universidade Santo Amaro(ブラジル)

    • Related Report
      2020 Research-status Report
  • [Journal Article] Development of a novel real-time polymerase chain reaction assay to detect Escherichia albertii in chicken meat.2022

    • Author(s)
      Sakura Arai, Tadasuke Ooka, Mizuha Shibata, Yuhki Nagai, Yuki Tokoi, Hiromi Nagaoka, Rika Maeda, Akihiko Tsuchiya, Yuka Kojima, Kenji Ohya, Takahiro Ohnishi, Noriko Konishi, Kayoko Ohtsuka, Yukiko Hara-Kudo.
    • Journal Title

      Foodborne Pathogens and Disease

      Volume: 19(12) Issue: 12 Pages: 823-829

    • DOI

      10.1089/fpd.2022.0042

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Escherichia albertii in retail oysters.2022

    • Author(s)
      Sakura Arai, Satoko Yamaya, Kayoko Ohtsuka, Noriko Konishi, Hiromi Obata, Tadasuke Ooka, Shouhei Hirose, Akemi Kai, Yukiko Hara-Kudo
    • Journal Title

      Journal of food protection

      Volume: 85(1) Issue: 1 Pages: 173-179

    • DOI

      10.4315/jfp-21-222

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Diversification of Escherichia albertii H-Antigens and Development of H-Genotyping PCR2021

    • Author(s)
      Nakae Koji、Ooka Tadasuke、Murakami Koichi、Hara-Kudo Yukiko、Imuta Naoko、Gotoh Yasuhiro、Ogura Yoshitoshi、Hayashi Tetsuya、Okamoto Yasuhiro、Nishi Junichiro
    • Journal Title

      Frontiers in Microbiology

      Volume: 12 Pages: 737979-737979

    • DOI

      10.3389/fmicb.2021.737979

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genomic properties and temporal analysis of the interaction of an invasive Escherichia albertii with epithelial cells.2020

    • Author(s)
      Fabiano T Romao, Fernando Henrique Martins, Rodrigo Tavanelli Hernandes, Tadasuke Ooka, Fernanda Fernandes Dos Santos, Denise Yamamoto, Alexis Bonfim-Melo, Tetsuya Hayashi, Waldir P Elias, Vanessa Sperandio, Tania Aparecida Tardelli Gomes
    • Journal Title

      Frontiers Cellular and Infection Microbiology

      Volume: 10 Pages: 571088-571088

    • DOI

      10.3389/fcimb.2020.571088

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Escherichia albertii Pathogenesis2020

    • Author(s)
      Tania Gomes, Tadasuke Ooka, Rodrigo Hernandes, Denise Yamamoto, Tetsuya Hayashi
    • Journal Title

      EcoSal Plus

      Volume: 9(1) Issue: 1

    • DOI

      10.1128/ecosalplus.esp-0015-2019

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 新興下痢症起因菌Escherichia albertiiのintimin/Tir/TccPの多様性とその分布2022

    • Author(s)
      大岡 唯祐、藺牟田直子、林 哲也、西 順一郎
    • Organizer
      第95回日本細菌学会総会
    • Related Report
      2021 Research-status Report
  • [Presentation] 新興下痢感染症 Escherichia albertii の鞭毛 H 抗原遺伝子型の多様性と遺伝子型別法開発への応用2022

    • Author(s)
      中江 広治、大岡 唯祐、村上 光一、工藤 由起子、藺牟田 直子、後藤 恭宏、小椋義俊、林 哲也、岡本康裕、西 順一郎
    • Organizer
      第95回日本細菌学会総会
    • Related Report
      2021 Research-status Report
  • [Presentation] 新興下痢症起因菌Escherichia albertiiのH抗原多様性と マルチプレックスPCRによるH抗原ジェノタイピング法の開発2021

    • Author(s)
      大岡 唯祐、中江 広治、村上 光一、工藤 由起子、藺牟田 直子、後藤 恭宏、小椋義俊、林 哲也、岡本康裕、西 順一郎
    • Organizer
      第42回日本食品微生物学会学術総会
    • Related Report
      2021 Research-status Report
  • [Presentation] 新興下痢症起因菌Escherichia albertiiによる最近の食中毒の状況と検査法2020

    • Author(s)
      大岡唯祐
    • Organizer
      令和2年度鹿児島県獣医公衆衛生講習会
    • Related Report
      2020 Research-status Report
    • Invited

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Published: 2020-04-28   Modified: 2024-12-25  

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