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Basic study for tolerance induction using 1BS-18 wheat in the patients with wheat-dependent exercise-induced anaphylaxis

Research Project

Project/Area Number 20K08802
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 54020:Connective tissue disease and allergy-related
Research InstitutionShimane University

Principal Investigator

Morita Eishin  島根大学, 医学部, 客員研究員 (90182237)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords小麦依存性運動誘発アナフィラキシー / オメガ5-グリアジン / 低アレルゲン小麦 / 耐性獲得 / 根治療法 / 小麦アレルギー / ω-5グリアジン / 免疫寛容 / 好塩基球活性化試験 / 1BS-18ホクシン / 1BS-18ホクシンパン
Outline of Research at the Start

1BS-18ホクシンパンによる経口減感作の成立を基礎的に裏付ける根拠を得ることを目的として、成人小麦アレルギー患者の1BS-18ホクシンパンによる減感作療法の前後に、患者末梢血の免疫担当細胞の動態を観察する。検討項目は、血清ω-5グリアジン特異的IgEおよびIgG4、小麦抗原による末梢血好塩基球活性化、1BS-18ホクシン小麦添加による末梢血単核球からのIL-4、IL-10、TGF-ベータの産生量、末梢血中の抑制性T細胞(Treg)の割合とする。患者における減感作療法の成否をこれらの免疫担当細胞の動態と比較検討することにより、減感作療法の成立機序を明らかにすることができると考えている。

Outline of Final Research Achievements

As a basic study to develop a tolerance for wheat allergy in adults, wheat allergen-specific IgE test and basophil activation test were investigated before and after continuous consumption of wheat strain 1 BS -18 bread lacking major wheat allergen omega 5-gliadin for 3 months. There was no significant reduction in either test. However, in a rat wheat allergy model, it was found that continuous intake of 1 BS -18 gluten in advance prevented sensitization by the administration of gluten of normal wheat or omega 5-gliadin. This indicates that T cell epitope of omega 5-gliadin in 1 BS -18 gluten is sufficient for the induction of tolerance.

Academic Significance and Societal Importance of the Research Achievements

小麦は成人の食物アレルギーでは頻度の高い原因食品で、多くが重篤な小麦依存性運動誘発アナフィラキシーとなる。小麦アレルギー患者は小麦摂取制限や食後の運動制限を強いられることになり、QOLが著しく低下している。申請者らは、小麦依存性運動誘発アナフィラキシーの主要アレルゲンであるω-5グリアジンを欠失したChinese Spring 1BS-18小麦系統を見出し、小麦依存性運動誘発アナフィラキシー患者が1BS-18系統の小麦を一定量継続摂取できることを確認した。さらにラットの小麦アレルギーモデルで、1BS-18小麦を継続摂取することで耐性を獲得する可能性を示した。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (9 results)

All 2023 2022 2021 2020

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (4 results) (of which Invited: 2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] A Narrative Mini Review on Current Status of Hypoallergenic Wheat Development for IgE-Mediated Wheat Allergy, Wheat-Dependent Exercise-Induced Anaphylaxis.2023

    • Author(s)
      Morita E, Matsuo H, Kohno K, Yokooji T, Yano H, Endo T.
    • Journal Title

      Foods

      Volume: 12 Issue: 5 Pages: 954-954

    • DOI

      10.3390/foods12050954

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hypoallergenic Wheat Line (1BS-18H) Lacking ω5-Gliadin Induces Oral Tolerance to Wheat Gluten Proteins in a Rat Model of Wheat Allergy.2022

    • Author(s)
      Yamada Y, Yokooji T, Kunimoto K, Inoguchi K, Ogino R, Taogoshi T, Morita E, Matsuo H.
    • Journal Title

      Foods

      Volume: 11 Issue: 15 Pages: 2181-2181

    • DOI

      10.3390/foods11152181

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome-wide association study reveals an association between the HLA-DPB1:02:01:02 allele and wheat-dependent exercise-induced anaphylaxis.2021

    • Author(s)
      Fukunaga K, Chinuki Y, Hamada Y, Fukutomi Y, Sugiyama A, Kishikawa R, Fukunaga A, Oda Y, Ugajin T, Yokozeki H, Harada N, Suehiro M, Hide M, Nakagawa Y, Noguchi E, Nakamura M, Matsunaga K, Yagami A, Morita E, Mushiroda T.
    • Journal Title

      Am J Hum Genet.

      Volume: 108 Issue: 8 Pages: 1540-1548

    • DOI

      10.1016/j.ajhg.2021.06.017

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of peroxidase-1 and beta-glucosidase as cross-reactive wheat allergens in grass pollen-related wheat allergy2021

    • Author(s)
      Ogino R, Chinuki Y, Yokooji T, Takizawa D, Matsuo H, Morita E
    • Journal Title

      Allergology International

      Volume: 70 Issue: 2 Pages: 215-222

    • DOI

      10.1016/j.alit.2020.09.005

    • NAID

      130008024312

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ω5-グリアジン欠損食用小麦の開発と普及に向けた取り組み2023

    • Author(s)
      森田栄伸
    • Organizer
      第23回食物アレルギー研究会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 感作経路から見える成人食物アレルギー2021

    • Author(s)
      森田栄伸
    • Organizer
      第120回日本皮膚科学会総会
    • Related Report
      2021 Research-status Report
  • [Presentation] 食物依存性運動誘発アナフィラキシーの病態に迫る.2020

    • Author(s)
      森田栄伸
    • Organizer
      第16回日本消化管学会総会学術集会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] イネ科花粉に感作され発症したと考えられる花粉-小麦アレルギーの交差性抗原解析.2020

    • Author(s)
      荻野龍平、千貫祐子、森田栄伸、横大路智治、滝沢大吾、松尾裕彰
    • Organizer
      日本花粉学会第61回大会
    • Related Report
      2020 Research-status Report
  • [Patent(Industrial Property Rights)] 低アレルゲン化小麦2021

    • Inventor(s)
      森田栄伸、河野邦江、遠藤 隆、松尾裕彰、横大路智治
    • Industrial Property Rights Holder
      森田栄伸、河野邦江、遠藤 隆、松尾裕彰、横大路智治
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-004081
    • Filing Date
      2021
    • Related Report
      2021 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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