• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

mesenchymal stem cells with antifibrotic effect prevent impairment of cardiac function after myocardial infarction

Research Project

Project/Area Number 20K09149
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 55030:Cardiovascular surgery-related
Research InstitutionHiroshima University

Principal Investigator

Takahashi Shinya  広島大学, 医系科学研究科(医), 教授 (70423382)

Co-Investigator(Kenkyū-buntansha) 中島 歩  広島大学, 医系科学研究科(医), 共同研究講座教授 (40448262)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2022: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords心筋梗塞 / 間葉系幹細胞 / 低酸素培養 / 無血清培地 / ラット心筋梗塞モデル / 抗炎症作用 / 心筋虚血 / 無血性培地 / 抗炎症性 / 抗線維化
Outline of Research at the Start

心筋梗塞後の心機能低下の一因として、心筋の線維化がある。間葉系幹細胞:Mesenchymal Stem Cells (MSC) の心筋梗塞に対する効果は一定の見解が得られているが、未だ十分な結果が得られていないのが実際である。本研究では、抗炎症効果および抗線維化作用を有するMSCの心筋梗塞モデルに対する作用を種々の蛋白発現と心機能の変化を比較検討することにより、そのメカニズムを明らかにしようとするものである。

Outline of Final Research Achievements

We compared the therapeutic effects of bone marrow-derived MSCs and adipose-derived MSCs on rat myocardial infarction models. We judged it appropriate to evaluate cardiac function in rat anterior descending artery ligation model using echocardiography and PV loop. Some modifications were required due to the extremely low survival rate of the model. For respiratory management, we devised a method to make mask ventilation airtight with high-viscosity gel, which reduced the mortality rate by half. A comparative study was conducted on the ligation model in 4 groups including bone marrow/fat-derived MSC administration. Adipose-derived MSCs had a high mortality rate of 80%. Another problem was the stability of the model. Even with the same LAD ligation procedure, there were variations in the amount of myocardial infarction, and it was considered necessary to re-verify the study up to the previous year.

Academic Significance and Societal Importance of the Research Achievements

ラットないしマウスを用いた心筋梗塞モデルの実験系は広く行われているものの、ときに論文に認められる方法論の再現性が疑わしいことがあり、本研究での成果は今だ実験系の確立に至るまでの部分が多くを占めるが、これから実験を行う研究者における学術的意義は高いと考えられる。まだその検討は始められたばかりと言わざるを得ないが、低酸素培養による間葉系幹細胞培養は、心筋梗塞の縮小効果を示す可能性があり、その研究を継続することは社会的意義があると考えられる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (4 results)

All 2022 2021

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (1 results)

  • [Journal Article] Neural network-based modeling of the number of microbubbles generated with four circulation factors in cardiopulmonary bypass2021

    • Author(s)
      Miyamoto Satoshi、Soh Zu、Okahara Shigeyuki、Furui Akira、Takasaki Taiichi、Katayama Keijiro、Takahashi Shinya、Tsuji Toshio
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 549-549

    • DOI

      10.1038/s41598-020-80810-3

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Serum-free medium and hypoxic preconditioning synergistically enhance the therapeutic effects of mesenchymal stem cells on experimental renal fibrosis2021

    • Author(s)
      Ishiuchi N, Nakashima A, Doi S, Kanai R, Maeda S, Takahashi S, Nagao M, Masaki T.
    • Journal Title

      Stem Cell Res Ther

      Volume: 12 Issue: 1 Pages: 472-472

    • DOI

      10.1186/s13287-021-02548-7

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Human bone marrow-derived mesenchymal stromal cells cultured in serum-free media demonstrate enhanced antifibrotic abilities via prolonged survival and robust regulatory T cell induction in murine bleomycin-induced pulmonary fibrosis2021

    • Author(s)
      Takao Shun、Nakashima Taku、Masuda Takeshi、Namba Masashi、Sakamoto Shinjiro、Yamaguchi Kakuhiro、Horimasu Yasushi、Miyamoto Shintaro、Iwamoto Hiroshi、Fujitaka Kazunori、Hamada Hironobu、Takahashi Shinya、Nakashima Ayumu、Hattori Noboru
    • Journal Title

      Stem Cell Research & Therapy

      Volume: 12 Issue: 1 Pages: 506-506

    • DOI

      10.1186/s13287-021-02574-5

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 損傷脊髄における模擬微小重力培養間葉系幹細胞の毛細血管への影響.2022

    • Author(s)
      黒瀬智之, 高橋信也, 大塚貴志, 中川 慧, 河原裕美, 弓削 類
    • Organizer
      第21回日本再生医療学会総会
    • Related Report
      2022 Annual Research Report

URL: 

Published: 2020-04-28   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi