| Project/Area Number |
20K09274
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 55060:Emergency medicine-related
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| Research Institution | Nippon Medical School |
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Principal Investigator |
Yokobori Shoji 日本医科大学, 大学院医学研究科, 大学院教授 (70449271)
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| Co-Investigator(Kenkyū-buntansha) |
須田 智 埼玉医科大学, 医学部, 教授 (00366733)
佐々木 和馬 獨協医科大学, 医学部, 助教 (30832266)
山田 真吏奈 日本体育大学, 保健医療学部, 准教授 (70508621)
阪本 太吾 日本医科大学, 医学部, 助教 (90587073)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 心停止後症候群 / びまん性脳損傷 / ラットモデル / 頭部外傷 / 脳損傷 / 神経幹細胞移植 / 行動実験 / 蘇生後脳症 / 神経再生医療 |
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| Outline of Research at the Start |
①蘇生後脳症モデルにおいて神経幹細胞（SB623）移植の有効性を検討する
②幹細胞移植のIn situ transplantationと、経鼻的接種との比較を行い、行動実験および病理学的、電気生理学的有効性を明確にする。
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| Outline of Final Research Achievements |
In this study, we were able to establish and stabilize a rat model of post-cardiac arrest. Specifically, we conducted cannulation of the rat's tail artery under anesthesia, followed by endotracheal intubation. We then administered muscle relaxants to suppress spontaneous breathing, clamped the tube to induce asphyxia, and resumed ventilation after 5 minutes. Subsequently, we successfully created a post-cardiac arrest model by performing chest compressions to restore cardiac activity.
Furthermore, we are in the stage of considering therapeutic approaches such as neural stem cells and neurotrophic factors. For example, we are currently transplanting neural stem cells (San-bio SB-623) stereotactically into the brain ventricle and observing the recovery of neural function. Additionally, we aim to further establish precise behavioral assessment experiments and refine techniques for cell transplantation.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究はラット蘇生後脳症モデルを作成し、ヒト骨髄由来間葉系幹細胞（SB623）を経鼻的に投与する。in vivoによる電気生理学的評価、行動実験および組織学的評価を行うことで、細胞医薬による神経組織再生・保護が蘇生後脳症に対する機能転帰の改善に貢献するかを検討する。本研究は蘇生後脳症の治療において低侵襲的な細胞治療を応用する初めての試みとなった。
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