Elucidation of the mechanism by which tooth loss causes cognitive decline
| Project/Area Number |
20K10035
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57050:Prosthodontics-related
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| Research Institution | Hiroshima University |
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Principal Investigator |
Oue Hiroshi 広島大学, 医系科学研究科(歯), 助教 (70711307)
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| Co-Investigator(Kenkyū-buntansha) |
竹田 洋輔 北海道医療大学, 歯学部, 助教 (80812560)
横井 美有希 広島大学, 病院(歯), 助教 (90826869)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 歯の喪失 |
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| Outline of Research at the Start |
本研究ではまず，in vitroでBDNFが脳血管機能へ及ぼすメカニズムを検討する。主に脳血管内皮細胞のタイトジャンクションに関する解析を行う。次にマウスを用いた実験により歯の喪失が脳血管機能へ及ぼす影響を検討するため，行動学的実験を行う。これらの結果を総括して歯の喪失がＡＤなどの認知症と同様の脳血管障害を引き起こすメカニズムを分子生物学的に解明し，歯の喪失の予防や口腔の機能維持が全身の健康に及ぼす影響を明らかにする。
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| Outline of Final Research Achievements |
The purpose of this study was to elucidate the molecular biological mechanism by which tooth loss causes cerebrovascular disorders similar to AD and other dementias. We created an experimental group in which experimental tooth loss was induced by extraction of mouse molars, and oral functions were impaired. After the observation period, behavioral experiments such as the Barnes maze were conducted to measure learning and memory abilities. Brain tissue was then collected and subjected to Western blot analysis and immunohistochemical staining.
The results revealed that experimental tooth loss reduced learning and memory performance and affected the tight junctions of the blood-brain barrier (BBB), which is important for cerebrovascular function. This is the first report of the effects of tooth loss on the BBB and is a new finding.
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| Academic Significance and Societal Importance of the Research Achievements |
歯の喪失と認知機能が関連することは従来疫学研究で示されているが，そのメカニズムは不明である。アルツハイマー病(AD)など認知症患者の多くは脳血管障害を伴うことが明らかとなってきたことから，歯の喪失が認知機能低下を引き起こすメカニズムを脳血管機能に着目して明らかにできるのではと着想した。実験的な歯の喪失により，学習記憶能が低下し，脳血管機能に重要な血液脳関門（BBB）のタイトジャンクションに影響を与えることが明らかとなった。歯の喪失によるBBBへの影響を報告はこれまでになく，新たな知見が得られた。
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Report
(4 results)
Research Products
(3 results)
