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Bone regeneration by ultrasonic treatment with electrized water for dense bone

Research Project

Project/Area Number 20K10186
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57060:Surgical dentistry-related
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

MURATA Masaru  北海道医療大学, 歯学部, 教授 (00260662)

Co-Investigator(Kenkyū-buntansha) 根津 尚史  北海道医療大学, 歯学部, 准教授 (40264056)
斎藤 隆史  北海道医療大学, 歯学部, 教授 (40265070)
建部 廣明  北海道医療大学, 歯学部, 講師 (40638293)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords緻密骨 / 硬化骨 / 超音波 / 電解水 / 骨誘導 / 骨再生 / 強酸性電解水 / 超音波脱灰 / 骨形成 / 緻密硬化骨 / 再生
Outline of Research at the Start

難治性骨疾患では皮質骨が高度に石灰化して代謝活性の低い骨質になり,骨髄腔の狭小化と線維化で骨髄細胞に乏しくなる.生理的加齢でも皮質骨は緻密度が増加する.従来,下顎骨骨髄炎に対して骨穿孔法が施行されているが,最新のメカノケミカル(超音波,電解水)技術を融合させて,硬化緻密骨の骨質改善による骨誘導促進法と新規加工移植骨の開発に挑戦する.実験対象は成体後期のリタイヤラット頭頂骨で頭頂骨は皮質骨2層(内板と外板)で構成された緻密骨である.特に緻密骨への超音波によるナノマイクロバブル作用と酸性電解水による脱灰処理効果に着目して,処理骨をSEMとEDSで分析し,骨誘導再生プロセスを細胞生物学的に評価する.

Outline of Final Research Achievements

Ultrasonically scratched dense bone plate treated with acidic electrolyzed water (AEW)induced bone at 2 weeks after the graft in rat subcutaneous tissues. The bone-induced region was in the compartment (space like closed room) of AEW bone. The 3D space contributes maintenance of growth factors's concentration and its increase. In addition, the bone-demineralization by acidic electrolyzed water(AEW) expose collagen fibers (RGD sequence). We believe mesenchymal cells attach to the exposed collagen fibers as cell anchorage, and react with bone morphogenetic proteins from bone matrix, proliferate and differentiate into osteoblasts.
On the other hand, fresh dense bone, perforated dense bone and dense bone plate treated with AED did not induce bone and cartilage until 8 weeks.

Academic Significance and Societal Importance of the Research Achievements

新鮮骨移植がゴールドスタンダードと信じられているが加工処理骨の方が骨誘導能が高いことが明らかになった.特に緻密硬化骨は構造と骨質が悪いため,骨代謝・リモデリングに調和して組み込まれない.緻密硬化骨で骨再生を早期に起こすには超音波スケーラーで骨構造の改質と酸処理でコラーゲンを露出させる処理のコンビネーションが効果的である.
新鮮骨神話が崩れ,21世紀はチェアーサイドでの本技術による加工骨が移植骨のゴールドスタンダードになるであろう.

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (10 results)

All 2023 2022 2021

All Journal Article (8 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 8 results,  Open Access: 8 results) Presentation (2 results)

  • [Journal Article] Human dentin materials for minimally invasive bone regeneration: Animal studies and clinical cases2023

    • Author(s)
      Murata Masaru、Nezu Takashi、Takebe Hiroaki、Hirose Yukito、Okubo Naoto、Saito Takashi、Akazawa Toshiyuki
    • Journal Title

      Journal of Oral Biosciences

      Volume: 65 Issue: 1 Pages: 13-18

    • DOI

      10.1016/j.job.2022.10.003

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Primary Teeth-Derived Demineralized Dentin Matrix Autograft for Unilateral Maxillary Alveolar Cleft during Mixed Dentition2022

    • Author(s)
      Matsuzawa Yusuke、Okubo Naoto、Tanaka Soichi、Kashiwazaki Haruhiko、Kitagawa Yoshimasa、Ohiro Yoichi、Mikoya Tadashi、Akazawa Toshiyuki、Murata Masaru
    • Journal Title

      Journal of Functional Biomaterials

      Volume: 13 Issue: 3 Pages: 153-153

    • DOI

      10.3390/jfb13030153

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autograft of Demineralized Dentin Matrix Prepared Immediately after Extraction for Horizontal Bone Augmentation of the Anterior Atrophic Maxilla: A First Case of Non-Vital Tooth-Derived Dentin2022

    • Author(s)
      Naoto Okubo, Masahiro Ishikawa, Mamata Shakya, Hidetaka Hosono, Osamu Maehara, Tatsuya Ohkawara, Shunsuke Ohnishi, Toshiyuki Akazawa, Masaru Murata
    • Journal Title

      Journal of Hard Tissue Biology

      Volume: 31 Issue: 1 Pages: 47-54

    • DOI

      10.2485/jhtb.31.47

    • NAID

      130008149070

    • ISSN
      1341-7649, 1880-828X
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Osteoinduction in Novel Micropores of Partially Dissolved and Precipitated Hydroxyapatite Block in Scalp of Young Rats2021

    • Author(s)
      Murata M, Hino J, Kabir MA, Yokozeki K, Sakamoto M, Nakajima T, Akazawa T.
    • Journal Title

      Materials (Basel)

      Volume: 14 Issue: 1 Pages: 196-204

    • DOI

      10.3390/ma14010196

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Human Dentin-Derived Biomaterial in Sheep Critical-Size Iliac Defects.2021

    • Author(s)
      Kabir MA, Murata M, Shakya M, Yamada K, Akazawa T.
    • Journal Title

      Materials (Basel)

      Volume: 14 Issue: 1 Pages: 223-223

    • DOI

      10.3390/ma14010223

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mechanical Properties of Human Concentrated Growth Factor (CGF) Membrane and the CGF Graft with Bone Morphogenetic Protein-2 (BMP-2) onto Periosteum of the Skull of Nude Mice.2021

    • Author(s)
      Kabir MA, Hirakawa A, Zhu B, Yokozeki K, Shakya M, Huang B, Akazawa T, Todoh M, Murata M.
    • Journal Title

      Int J Mol Sci.

      Volume: 22(21) Issue: 21 Pages: 11331-11331

    • DOI

      10.3390/ijms222111331

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Accelerated Bone Induction of Adult Rat Compact Bone Plate Scratched by Ultrasonic Scaler Using Acidic Electrolyzed Water.2021

    • Author(s)
      Shakya M, Murata M, Yokozeki K, Akazawa T, Nagayasu H, Adhikari BR, Upadhyaya C.
    • Journal Title

      Materials (Basel)

      Volume: 14(12) Issue: 12 Pages: 3347-3347

    • DOI

      10.3390/ma14123347

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Chemical Properties of Human Dentin Blocks and Vertical Augmentation by Ultrasonically Demineralized Dentin Matrix Blocks on Scratched Skull without Periosteum of Adult-Aged Rats.2021

    • Author(s)
      Zhu B, Yokozeki K, Kabir MA, Todoh M, Akazawa T, Murata M.
    • Journal Title

      Materials (Basel)

      Volume: 15(1) Issue: 1 Pages: 105-105

    • DOI

      10.3390/ma15010105

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] リタイヤラット頭蓋骨骨膜切除モデルにおける新規GBR膜の効果2022

    • Author(s)
      村田 勝,横関 健治,草野 薫
    • Organizer
      第51回日本口腔インプラント学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] リン酸オクタカルシウム・アテロコラーゲンディスクへのBMP-2添加による骨誘導 ―凍結緻密骨との比較―2022

    • Author(s)
      横関健治,朱 博文,Md. A Kabir,甲斐壮馬,松林秀繁,村田 勝
    • Organizer
      第76回日本口腔科学会
    • Related Report
      2022 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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