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Identification of brain regions and neural circuits involved in defecation control by the central nervous system and analysis of their functions

Research Project

Project/Area Number 20K16120
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 48020:Physiology-related
Research InstitutionKagoshima University

Principal Investigator

NAITOU Kiyotada  鹿児島大学, 農水産獣医学域獣医学系, 准教授 (30794903)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Discontinued (Fiscal Year 2022)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2022: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords脊髄 / 排便 / 脳 / 消化管 / 中枢神経
Outline of Research at the Start

近年、ストレスによる排便障害が大きな問題となっている。中枢神経系に原因があると考えられているが、中枢神経系による排便の制御メカニズムは未だ謎に包まれており、ストレスによる排便障害のの病態は明らかになっていない。本研究では、排便制御に関わる神経領域および神経回路を同定し、その役割を明らかにすることを目的とする。これにより、ストレスによる排便障害の病態の解明および治療法の開発に貢献したい。

Outline of Final Research Achievements

In this study, we used retrograde tracers to identify brain regions involved in defecation control. Adeno-associated viral vectors expressing fluorescent proteins in infected neurons were injected into the lumbosacral spinal cord, where the spinal defecation center is located, and the neurons projecting to the spinal defecation center were labeled with fluorescent proteins. Tissue sections were prepared and brain regions with fluorescent protein-labeled nerves were searched. The characteristics of the fluorescent protein-labeled nerves were further investigated by immunofluorescence staining. We also searched for neurotransmitters that act in the spinal cord and examined their mechanisms of action. These results were presented in scientific papers in English and at domestic conferences.

Academic Significance and Societal Importance of the Research Achievements

本研究結果から、排便制御に関わる脳領域の一部が示唆された。また、脊髄排便中枢で作用する神経伝達物質が複数明らかになった。これまで知られていなかった、脊髄排便中枢で作用する神経伝達物質が明らかになったことで、脊髄排便中枢における排便制御メカニズムの一端が明らかになった。このことは、中枢神経系による排便制御の仕組みを解明するために必要な基礎的な情報を提供し、この分野の研究の進展に貢献するものだと考えている。また、本研究の成果は、ストレス性の腹痛や下痢など、中枢神経系が深く関わる排便障害の病態を解明するために必要となる、基礎的な情報を提供しており、社会的な意義も大きい。

Report

(3 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Research-status Report
  • Research Products

    (8 results)

All 2022 2021 2020

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (4 results) (of which Invited: 1 results)

  • [Journal Article] Intrathecally administered substance P activated the spinal defecation center and enhanced colorectal motility in anesthetized rats2022

    • Author(s)
      Naitou Kiyotada、Iwashita Honoka、Ueda Hiromi H.、Shiraishi Mitsuya、Fujimoto Yoshikazu、Horii Kazuhiro、Sawamura Tomoya、Shiina Takahiko、Shimizu Yasutake
    • Journal Title

      American Journal of Physiology-Gastrointestinal and Liver Physiology

      Volume: 323 Issue: 1 Pages: G21-G30

    • DOI

      10.1152/ajpgi.00342.2021

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of sex hormones to the sexually dimorphic response of colorectal motility to noxious stimuli in rats2022

    • Author(s)
      Horii Kazuhiro、Sawamura Tomoya、Onishi Ayaka、Yuki Natsufu、Naitou Kiyotada、Shiina Takahiko、Shimizu Yasutake
    • Journal Title

      American Journal of Physiology-Gastrointestinal and Liver Physiology

      Volume: 323 Issue: 1 Pages: G1-G8

    • DOI

      10.1152/ajpgi.00033.2022

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] α-MSH-induced activation of spinal MC1R but not MC4R enhances colorectal motility in anaesthetised rats2021

    • Author(s)
      Ueda Hiromi H.、Naitou Kiyotada、Nakamori Hiroyuki、Horii Kazuhiro、Shiina Takahiko、Masatani Tatsunori、Shiraishi Mitsuya、Shimizu Yasutake
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 487-487

    • DOI

      10.1038/s41598-020-80020-x

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sexually dimorphic response of colorectal motility to noxious stimuli in the colorectum in rats2021

    • Author(s)
      Horii Kazuhiro、Ehara Yuka、Shiina Takahiko、Naitou Kiyotada、Nakamori Hiroyuki、Horii Yuuki、Shimaoka Hiroki、Saito Shouichiro、Shimizu Yasutake
    • Journal Title

      The Journal of Physiology

      Volume: 599 Issue: 5 Pages: 1421-1437

    • DOI

      10.1113/jp279942

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 排便制御に関わる中枢神経系の神経回路の解明2021

    • Author(s)
      内藤清惟
    • Organizer
      第164回日本獣医学会学術集会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 脊髄排便中枢におけるサブスタンスPの大腸運動促進作用2021

    • Author(s)
      内藤清惟、岩下朋乃樺、植田大海、白石光也、藤本佳万、椎名貴彦、志水泰武
    • Organizer
      第164回日本獣医学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Activation of melanocortin 1 receptor in the spinal defecation center enhanced colorectal motility in rats.2021

    • Author(s)
      Kiyotada NAITOU, Hiromi UEDA, Mitsuya SHIRAISHI, Tatsunori MASATANI, Hiroyuki NAKAMORI, Kazuhiro Horii, Takahiko SHIINA, Yasutake SHIMIZU
    • Organizer
      第126回日本解剖学会総会・全国学術集会 / 第98回日本生理学会大会 合同大会
    • Related Report
      2020 Research-status Report
  • [Presentation] in vivo大腸運動記録を用いたα-MSHによる排便促進作用メカニズムの検討2020

    • Author(s)
      植田大海、内藤清惟、白石光也、中森裕之、椎名貴彦、志水泰武
    • Organizer
      第10回 名古屋大学医学系研究科・生理学研究所合同シンポジウム
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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