| Project/Area Number |
20K16262
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 49060:Virology-related
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| Research Institution | Kanazawa University |
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Principal Investigator |
Lim Kee Siang 金沢大学, ナノ生命科学研究所, 特任助教 (60842987)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | Viral fusion proteins / HS-AFM / Hemagglutinin / Spike protein / SARS-CoV-2 / Influenza A / HS-AFM nanoimaging / viral fusion protein / SARS-CoV-2 spike protein / High-speed AFM / Neutralizing antibodies / COVID-19 / Viral Fusion Protein / Viral fusion protein / fusogenic transition / exosome / Direct visualization / Virulence factors |
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| Outline of Research at the Start |
HS-AFM is an ideal tool for investigating properties of viral fusion proteins that include native conformation, binding activity with host factors, and structural dynamic during fusogenic transition. Furthermore, interaction between fusion proteins and exosomes will be also be recorded.
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| Outline of Final Research Achievements |
The applicant has investigated molecular properties of two viral fusion proteins: influenza A hemagglutinin (HA) and SARS-CoV-2 spike protein (S protein) using HS-AFM. Native conformation and dynamic behavior of HA, particularly the fusogenic transition, and the interaction between lipid layer and HA have been recorded using HS-AFM. Likewise, structural dynamics of S protein, S protein-ACE2 protein interaction, S protein-exosome interaction, and S protein-neutralizing interaction, have been visualized and analyzed using HS-AFM. The results have been reported in high impact journals.
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| Academic Significance and Societal Importance of the Research Achievements |
HS-AFM imaging allows the applicant to study structural dynamics of viral proteins and dynamic viral protein-antibody interactions, in which it is difficult to do using conventional methods such as cryo-EM. The applicant’s studies have been reported in newspapers and on news television channels.
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