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Gene expression analysis of affected blood vessels in moyamoya disease

Research Project

Project/Area Number 20K17961
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 56010:Neurosurgery-related
Research InstitutionNagoya University

Principal Investigator

Yokoyama Kinya  名古屋大学, 医学部附属病院, 助教 (90867904)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2022: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsmoyamoya disease / transcriptome / Microarray / messenger RNA / long non-coding RNA / もやもや病 / マイクロアレイ / 遺伝子発現解析
Outline of Research at the Start

もやもや病は頭蓋内動脈の進行性狭窄と血管新生を特徴とする原因不明の疾患である。今までにマイクロアレイをはじめとした病変部の遺伝子発現解析は殆ど行われていないが、我々は既存の試薬キットを用いて、脳表の中大脳動脈から抽出したtotal RNAより増幅compliment DNAを作成し、マイクロアレイによる網羅的な遺伝子発現解析と定量PCRを実施するプロトコールを確立した。今研究の目的は、マイクロアレイを用いて、成人・小児を含めたもやもや病患者の病変部の網羅的な遺伝子発現解析を行い、非もやもや病患者検体との比較により、もやもや病患者の病変部に特異的な発現遺伝子・パスウェイの特定を行うことである。

Outline of Final Research Achievements

During the study period, 21 patients with Moyamoya disease, 6 patients with aneurysms and 5 patients with intractable epilepsy were sampled from the brain surface arteries and subjected to comprehensive gene expression analysis using microarrays. Comprehensive gene expression analysis for messenger RNA was performed on 11 moyamoya disease patients, 6 aneurysms as a control group, and 3 intractable epilepsy patients. In addition, we comprehensively analyzed long non-coding RNA expression in the intracranial artery walls of 21 patients with moyamoya disease and 11 patients with non-moyamoya disease, and published the results in a paper.

Academic Significance and Societal Importance of the Research Achievements

もやもや病の網羅的遺伝子発現解析については、これまで循環血中やiPS細胞については行われてきたが、病変部血管に対するものとしては我々の研究が初となった。これまでの研究では炎症や免疫応答の関与が推察されてきたが、今研究により、もやもや病の病変部である、脳動脈血管壁においても、同様の変化が生じていることが示された。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (7 results)

All 2023 2022 2021

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Long noncoding RNA profile of the intracranial artery in patients with moyamoya disease2023

    • Author(s)
      Mamiya Takashi、Kanamori Fumiaki、Yokoyama Kinya、Ota Akinobu、Karnan Sivasundaram、Uda Kenji、Araki Yoshio、Maesawa Satoshi、Yoshikawa Kazuhiro、Saito Ryuta
    • Journal Title

      Journal of Neurosurgery

      Volume: 138 Issue: 3 Pages: 709-716

    • DOI

      10.3171/2022.5.jns22579

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transcriptome-wide analysis of intracranial artery in patients with moyamoya disease showing upregulation of immune response, and downregulation of oxidative phosphorylation and DNA repair2021

    • Author(s)
      Kanamori Fumiaki、Yokoyama Kinya、Ota Akinobu、Yoshikawa Kazuhiro、Karnan Sivasundaram、Maruwaka Mikio、Shimizu Kenzo、Ota Shinji、Uda Kenji、Araki Yoshio、Okamoto Sho、Maesawa Satoshi、Wakabayashi Toshihiko、Natsume Atsushi
    • Journal Title

      Neurosurgical Focus

      Volume: 51 Issue: 3 Pages: E3-E3

    • DOI

      10.3171/2021.6.focus20870

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] RNF213 p.4810K variantがもやもや病頭蓋内動脈の長鎖ノンコーディングRNA発現に与える影響2023

    • Author(s)
      金森史哲, 横山欣也
    • Organizer
      STROKE2023
    • Related Report
      2022 Annual Research Report
  • [Presentation] Comparing the Transcriptome Profile of the Middle Cerebral Artery between the RNF213 genotypes in the Patients with Moyamoya disease2023

    • Author(s)
      Fumiaki Kanamori, Kinya Yokoyama
    • Organizer
      The 10th Pan-Pacific Neurosurgical Congress oral presentation
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparing the Transcriptome Profile of the Middle Cerebral Artery between the RNF213 genotypes in the Patients with Moyamoya disease2022

    • Author(s)
      Fumiaki Kanamori, Kinya Yokoyama
    • Organizer
      World Internet Conference on Moyamoya Disease
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] もやもや病患者脳動脈におけるトランスクリプトーム解析-RNF213 p.R4810K variantによる影響の検討2021

    • Author(s)
      横山 欣也
    • Organizer
      日本脳神経外科学会第80回学術総会
    • Related Report
      2021 Research-status Report
  • [Presentation] もやもや病頭蓋内動脈の網羅的遺伝子発現解析2021

    • Author(s)
      金森 史哲, 横山 欣也
    • Organizer
      日本脳神経外科学会第80回学術総会
    • Related Report
      2021 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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