Vascular network as a basis for dental pulp stem cell niche formation

Research Project

Project/Area Number	20K18544
Research Category	Grant-in-Aid for Early-Career Scientists
Allocation Type	Multi-year Fund
Review Section	Basic Section 57030:Conservative dentistry-related
Research Institution	Tokyo Dental College
Principal Investigator	MITOMO KEISUKE 東京歯科大学, 歯学部, 助教 (90844051)
Project Period (FY)	2020-04-01 – 2023-03-31
Project Status	Completed (Fiscal Year 2022)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	歯髄 / Type H血管 / 毛細血管 / TypeH毛細血管 / 歯根膜 / CD31 / Endomucin / TypeH血管 / 血管ネットワーク
Outline of Research at the Start	歯髄において血管近傍に歯髄幹細胞が存在することが知られている。しかし歯髄幹細胞に対する歯髄血管の機能はおろか、歯髄の歯髄血管サブタイプの種類・局在はわかっていない。そこで本申請課題では、歯髄血管サブタイプを同定し、歯髄血管ネットワークの詳細を明らかにすることを目的とする。本研究の推進により、歯髄幹細胞ニッチの形成の解明の基礎になるだけでなく、象牙質再生・歯髄再生療法に関する研究の新たな基盤構築が期待できる。
Outline of Final Research Achievements	It was found that Type H vessels in the pulp exist, but that the amount of these vessels decreases over time. The relationship between Type H blood vessels and hard tissue formation was also examined. Wild-type C57BL/6J mice (4w, 8w, and 12w) were calcein-labeled and labeled with neoplastic hard tissues, which were then immunostained with anti-endomucin antibodies. The results showed that many Endomucin-positive vessels were observed in the pulp and dentin just below the calcein-labeled neocortical tissue. This suggests that Endomucin vessels contribute to hard tissue regeneration in the dental pulp. A paper based on these results is currently being written and will be submitted to an English journal.Translated with www.DeepL.com/Translator (free version)
Academic Significance and Societal Importance of the Research Achievements	今回の研究結果より、歯髄を構成する血管ネットワークは硬組織形成に寄与することが示唆された。これは、象牙質の形成や歯髄幹細胞ニッチ形成を解明するための基礎となると考えている。