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Investigation of transcriptional activity of microbiota for causing peri-implantitis

Research Project

Project/Area Number 20K18567
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 57040:Regenerative dentistry and dental engineering-related
Research InstitutionNihon University

Principal Investigator

WATANABE Takayasu  日本大学, 歯学部, 講師 (70725514)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsインプラント周囲炎 / 細菌叢 / メタトランスクリプトーム / 機械学習 / ディープラーニング
Outline of Research at the Start

インプラント治療の普及に伴い増加しているインプラント周囲炎の発症・進行をコントロールするには、細菌叢全体に目を向けて、集団としての活動態様を解明することが喫緊の課題であるが、本研究では、実際に転写されている遺伝子の種類や転写活動性を発揮している細菌の種類を知ることができ、特定の細菌種に絞って抗菌薬投与などで対処してきた従来の炎症治療法に代わる「対細菌叢」医療実現の基盤となる成果が期待される。

Outline of Final Research Achievements

Within microbiota around a dental implant, certain types of bacterial genes were highly expressed at the transcription level, whereas their expressional changes were not apparent during the passage of several weeks. On the other hand, bacterial taxa responsible for gene expression showed diversity among patients with respect to their proportions and changing patterns. These observations suggest that the bacterial gene expression changes with time passage within the peri-implant microbiota, whereas changes of bacterial taxa responsible for gene expression with time passage are diverse. In addition, samples of microbiota at oral disease lesions could be distinguished by applying machine learning.

Academic Significance and Societal Importance of the Research Achievements

インプラント周囲に生じる複合感染症であるインプラント周囲炎については、インプラント体を埋入した時点で炎症が生じていなくても、細菌叢において何らかの病的な変化を経ることによって炎症の発症につながることが示唆される。本研究で明らかにした、インプラント周囲細菌叢における細菌の遺伝子転写発現やそれを主体的に担う細菌の種類の時系列的な変化は、細菌叢が将来的に病的変化するとすればそのもととなる状態と位置づけることができ、インプラント周囲炎の発症・進行をコントロールする上での基礎的情報となり得る。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (13 results)

All 2022 2021 2020

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (8 results) (of which Int'l Joint Research: 2 results) Book (2 results)

  • [Journal Article] Patient‐specific establishment of bacterial composition within the peri‐implant microbiota during the earliest weeks after implant uncovering2021

    • Author(s)
      Shimogishi Masahiro、Watanabe Takayasu、Shibasaki Masaki、Shiba Takahiko、Komatsu Keiji、Nemoto Takashi、Ishihara Kazuyuki、Nakano Yoshio、Iwata Takanori、Kasugai Shohei、Nakagawa Ichiro
    • Journal Title

      Journal of Periodontal Research

      Volume: 56 Issue: 5 Pages: 964-971

    • DOI

      10.1111/jre.12898

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Discrimination of Bacterial Community Structures among Healthy, Gingivitis, and Periodontitis Statuses through Integrated Metatranscriptomic and Network Analyses2021

    • Author(s)
      Nemoto Takashi、Shiba Takahiko、Komatsu Keiji、Watanabe Takayasu、Shimogishi Masahiro、Shibasaki Masaki、Koyanagi Tatsuro、Nagai Takahiko、Katagiri Sayaka、Takeuchi Yasuo、Iwata Takanori
    • Journal Title

      mSystems

      Volume: 6 Issue: 6

    • DOI

      10.1128/msystems.00886-21

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Discriminating Microbial Community Structure Between Peri-Implantitis and Periodontitis With Integrated Metagenomic, Metatranscriptomic, and Network Analysis2020

    • Author(s)
      Komatsu Keiji、Shiba Takahiko、Takeuchi Yasuo、Watanabe Takayasu、Koyanagi Tatsuro、Nemoto Takashi、Shimogishi Masahiro、Shibasaki Masaki、Katagiri Sayaka、Kasugai Shohei、Iwata Takanori
    • Journal Title

      Frontiers in Cellular and Infection Microbiology

      Volume: 10 Pages: 596490-596490

    • DOI

      10.3389/fcimb.2020.596490

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] インプラント周囲細菌叢を規定するリスク因子の検討2022

    • Author(s)
      下岸将博、渡辺孝康、柴崎真樹、丸川恵理子
    • Organizer
      第26回日本顎顔面インプラント学会総会・学術大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 古代人の歯石DNAを用いた江戸時代と現代の網羅的口腔内細菌叢解析2022

    • Author(s)
      須藤毅顕、芝多佳彦、駒津匡二、澤藤りかい、佐宗亜衣子、植田信太郎、渡辺孝康、根本昂、加納千博、長井貴彦、大杉勇人、片桐さやか、竹内康雄、小林宏明、岩田隆紀
    • Organizer
      第76回日本人類学会大会・第38回日本霊長類学会大会連合大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] メタゲノム16S解析法を用いた難治性根尖性歯周炎症例における摘出検体の細菌叢解析2022

    • Author(s)
      佐竹和久、下岸将博、渡辺孝康、渡辺聡、興地隆史
    • Organizer
      第156回日本歯科保存学会学術大会(春季)
    • Related Report
      2022 Annual Research Report
  • [Presentation] 深層学習による歯周炎・インプラント周囲炎の識別方法の検討2021

    • Author(s)
      渡辺孝康,芝多佳彦,中野善夫
    • Organizer
      第63回歯科基礎医学会学術大会
    • Related Report
      2021 Research-status Report
  • [Presentation] インプラント周囲粘膜における遊離歯肉移植術が及ぼす細菌叢の変化2021

    • Author(s)
      柴崎真樹,下岸将博,渡辺孝康,丸川恵理子
    • Organizer
      第25回日本顎顔面インプラント学会総会・学術大会
    • Related Report
      2021 Research-status Report
  • [Presentation] メタトランスクリプトーム解析を用いた歯周疾患ステージにおける細菌種間のネットワーク構造と機能組成の比較2020

    • Author(s)
      根本昂,芝多佳彦,渡辺孝康,小柳達郎,駒津匡二,片桐さやか,竹内康雄,岩田隆紀
    • Organizer
      第152回日本歯科保存学会学術大会(春季)
    • Related Report
      2020 Research-status Report
  • [Presentation] Non-surgical treatment and microbiome of periodontitis and peri-implantitis: seven-year follow-up2020

    • Author(s)
      Komatsu K, Shiba T, Watanabe T, Nemoto T, Koyanagi T, Takeuchi Y, Iwata T
    • Organizer
      106th Annual Meeting of the American Academy of Periodontology in collaboration with the Japanese Society of Periodontology, and the Japanese Academy of Clinical Periodontology
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] Omics analysis defines differences in microbial community structure between peri-implantitis and periodontitis2020

    • Author(s)
      Komatsu K, Takeuchi Y, Shiba T, Watanabe T, Shimogishi M, Shibasaki M, Nemoto T, Koyanagi T, Katagiri S, Iwata T
    • Organizer
      68th Annual Meeting of Japanese Association for Dental Research
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Book] The Oral Microbiome2021

    • Author(s)
      Gopinath D, Menon RK, Hussein H, Tansirichaiya S, Reynolds LJ, Roberts AP, Kabwe M, Brown T, Ku H, Dashper S, Tucci J, Adami GR, Ang MJ, Kim EM, Marotz C, Zuniga C, Zaramela L, Knight R, Zengler K, Moore NE, Weyrich LS, Speicher DJ, Nasir JA, Zhou P, Anderson DE, Goh CE, Bohn B, Demmer RT, Watanabe T et al.
    • Total Pages
      295
    • Publisher
      Springer
    • Related Report
      2021 Research-status Report
  • [Book] Polymicrobial Peri-Implant Infection (in Ogata Y [Ed.] "Risk Factors for Peri-implant Diseases")2020

    • Author(s)
      Shiba T, Watanabe T
    • Total Pages
      120
    • Publisher
      Springer
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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