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Elucidating mechanisms of photosynthesis repression based on protein-oxidation system

Research Project

Project/Area Number 20K21268
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 38:Agricultural chemistry and related fields
Research InstitutionTokyo Institute of Technology

Principal Investigator

Yoshida Keisuke  東京工業大学, 科学技術創成研究院, 准教授 (40632310)

Project Period (FY) 2020-07-30 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2021: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2020: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords光合成 / レドックス制御 / タンパク質酸化 / チオレドキシン / チオレドキシン様タンパク質 / 葉緑体 / タンパク質
Outline of Research at the Start

酸化と還元を基盤としたタンパク質の機能制御であるレドックス制御は、植物の光合成の調節に必須の役割を果たしている。この制御系は古くから知られているものの、酸化側の制御を行うためのメカニズムはほとんど明らかになっていない。本研究では、研究代表者が最近成し遂げたタンパク質酸化因子の同定を突破口と捉え、タンパク質酸化システムの全容解明に挑む。光合成の抑制という新しい光合成制御機構を切り拓き、植物は夜どのように眠るのかという問いに対して1つの答えを提示することを目指す。

Outline of Final Research Achievements

In this study, we focused on the redox regulation system, which is the key to the control of plant photosynthesis. Based on the recent identification of protein-oxidation factor, I tried to uncover the oxidation side of redox regulation system, which has been a long-standing gap in the field. Through a series of biochemical and physiological studies, I have gained important insights into novel chloroplast functions activated by oxidation, the molecular machinery supporting the oxidation side of the redox regulation system, and the impact of oxidative regulation on plant growth. These results provide an important clue regarding how photosynthesis is suppressed during the night.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、「植物は変動する光環境の中で光合成をどのように制御して、持続的なバイオマス生産を成し遂げているのか」ということへの理解を、基礎科学の観点から確実に推し進めたものと言える。この知見は、光合成機能の強化による農作物の収量増加といった応用研究への展開のためにも、重要な情報を提供するものである。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (40 results)

All 2023 2022 2021 2020 Other

All Journal Article (15 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 15 results,  Open Access: 14 results) Presentation (21 results) (of which Int'l Joint Research: 3 results,  Invited: 7 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Cystathionine-β-synthase X proteins negatively regulate NADPH-thioredoxin reductase C activity2023

    • Author(s)
      Tran C.M., Mihara S., Yoshida K.*, Hisabori T.*
    • Journal Title

      Biochem. Biophysic. Res. Commun.

      Volume: 653 Pages: 47-52

    • DOI

      10.1016/j.bbrc.2023.02.055

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chloroplast translation factor EF-Tu of Arabidopsis thaliana can be inactivated via oxidation of a specific cysteine residue2023

    • Author(s)
      Toriu Machi、Horie Momoka、Kumaki Yuka、Yoneyama Taku、Kore-eda Shin、Mitsuyama Susumu、Yoshida Keisuke、Hisabori Toru、Nishiyama Yoshitaka
    • Journal Title

      Biochemical Journal

      Volume: 480 Issue: 5 Pages: 307-318

    • DOI

      10.1042/bcj20220609

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two specific domains of the γ subunit of chloroplast F <sub>o</sub> F <sub>1</sub> provide redox regulation of the ATP synthesis through conformational changes2023

    • Author(s)
      Akiyama Kentaro、Ozawa Shin-Ichiro、Takahashi Yuichiro、Yoshida Keisuke、Suzuki Toshiharu、Kondo Kumiko、Wakabayashi Ken-ichi、Hisabori Toru
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 120 Issue: 6

    • DOI

      10.1073/pnas.2218187120

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The ferredoxin/thioredoxin pathway constitutes an indispensable redox-signaling cascade for light-dependent reduction of chloroplast stromal proteins2022

    • Author(s)
      Yoshida K.*, Yokochi Y., Tanaka K., Hisabori T.
    • Journal Title

      J. Biol. Chem.

      Volume: 298 Issue: 12 Pages: 102650-102650

    • DOI

      10.1016/j.jbc.2022.102650

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dissipation of the proton electrochemical gradient in chloroplasts promotes the oxidation of ATP synthase by thioredoxin-like proteins2022

    • Author(s)
      Sekiguchi T., Yoshida K., Wakabayashi K-I., Hisabori T.*
    • Journal Title

      J. Biol. Chem.

      Volume: 298 Issue: 11 Pages: 102541-102541

    • DOI

      10.1016/j.jbc.2022.102541

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Verification of the Relationship between Redox Regulation of Thioredoxin Target Proteins and Their Proximity to Thylakoid Membranes2022

    • Author(s)
      Fukushi Y, Yokochi Y, Wakabayashi KI, Yoshida K, Hisabori T.
    • Journal Title

      Antioxidants

      Volume: 11 Issue: 4 Pages: 773-773

    • DOI

      10.3390/antiox11040773

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Importance of the C-terminal Cys pair of Phosphoribulokinase in Phototrophs in Thioredoxin-Dependent Regulation2022

    • Author(s)
      Fukui K, Yoshida K, Yokochi Y, Sekiguchi T, Wakabayashi KI, Hisabori T, Mihara S.
    • Journal Title

      Plant Cell Physiol.

      Volume: in press Issue: 6 Pages: 855-868

    • DOI

      10.1093/pcp/pcac050

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oxidative regulation of chloroplast enzymes by thioredoxin and thioredoxin-like proteins in <i>Arabidopsis thaliana</i>2021

    • Author(s)
      Yokochi Yuichi、Fukushi Yuka、Wakabayashi Ken-ichi、Yoshida Keisuke、Hisabori Toru
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 118 Issue: 51

    • DOI

      10.1073/pnas.2114952118

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The phototroph-specific β-hairpin structure of the γ subunit of FoF1-ATP synthase is important for efficient ATP synthesis of cyanobacteria2021

    • Author(s)
      Kondo Kumiko、Izumi Masayuki、Inabe Kosuke、Yoshida Keisuke、Imashimizu Mari、Suzuki Toshiharu、Hisabori Toru
    • Journal Title

      Journal of Biological Chemistry

      Volume: 297 Issue: 3 Pages: 101027-101027

    • DOI

      10.1016/j.jbc.2021.101027

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biochemical basis for redox regulation of chloroplast-localized phosphofructokinase from Arabidopsis thaliana2021

    • Author(s)
      Yoshida K., Hisabori T.
    • Journal Title

      Plant Cell Physiol.

      Volume: in press Issue: 3 Pages: 401-410

    • DOI

      10.1093/pcp/pcaa174

    • Related Report
      2021 Research-status Report 2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Redox regulation of NADP-malate dehydrogenase is vital for land plants under fluctuating light environment2021

    • Author(s)
      Yokochi Y., Yoshida K., Hahn F., Miyagi A., Wakabayashi K-I., Kawai-Yamada M., Weber A. P. M., Hisabori T.
    • Journal Title

      Proc. Natl. Acad. Sci. U.S.A.

      Volume: 118 Issue: 6

    • DOI

      10.1073/pnas.2016903118

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structural basis for thioredoxin isoform-based fine-tuning of ferredoxin-thioredoxin reductase activity2020

    • Author(s)
      Juniar L., Tanaka H., Yoshida K., Hisabori T., Kurisu G.
    • Journal Title

      Protein Sci.

      Volume: 29 Issue: 12 Pages: 2538-2545

    • DOI

      10.1002/pro.3964

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Biochemical insight into redox regulation of plastidial 3-phosphoglycerate dehydrogenase from Arabidopsis thaliana2020

    • Author(s)
      Yoshida K., Ohtaka K., Hirai M. Y., Hisabori T.
    • Journal Title

      J. Biol. Chem.

      Volume: 295 Issue: 44 Pages: 14906-14915

    • DOI

      10.1074/jbc.ra120.014263

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oxidation of aromatic and aliphatic aldehydes to carboxylic acids by Geotrichum candidum aldehyde dehydrogenase2020

    • Author(s)
      Hoshino Tomoyasu、Yamabe Emi、Hawari Muhammad Arisyi、Tamura Mayumi、Kanamaru Shuji、Yoshida Keisuke、Koesoema Afifa Ayu、Matsuda Tomoko
    • Journal Title

      Tetrahedron

      Volume: 76 Issue: 33 Pages: 131387-131387

    • DOI

      10.1016/j.tet.2020.131387

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chloroplast ATP synthase is reduced by both f-type and m-type thioredoxins2020

    • Author(s)
      Sekiguchi T., Yoshida K., Okegawa Y., Motohashi K., Wakabayashi K-I., Hisabori T.
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1861 Issue: 11 Pages: 148261-148261

    • DOI

      10.1016/j.bbabio.2020.148261

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 光合成のレドックス制御に関わるPost-Illumination Chlorophyll Fluorescence Increase (PIFI)タンパク質の分子機能解析2022

    • Author(s)
      村井美波,吉田啓亮,車玉芬,石川規子,久堀徹,伊福健太郎
    • Organizer
      第12回日本光合成学会年会およびシンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] Trx およびTrx 様タンパク質による葉緑体タンパク質の酸化制御2022

    • Author(s)
      福士結香,横地佑一,若林憲一,吉田啓亮,久堀徹
    • Organizer
      第12回日本光合成学会年会およびシンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] Functional interaction between Cystathionine-β-synthase X proteins and NADPH-thioredoxin reductase C in Arabidopsis thaliana2022

    • Author(s)
      Minh Chau Tran, Shoko Mihara, Ken-ichi Wakabayashi, Keisuke Yoshida, Toru Hisabori
    • Organizer
      第12回日本光合成学会年会およびシンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] Redox regulation system in chloroplasts; what we know about reduction and oxidation of target enzymes2022

    • Author(s)
      Toru Hisabori, Yuichi Yokochi, Takatoshi Sekiguchi, Yuka Fukushi, Ken-Ichi Wakabayashi, Keisuke Yoshida
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Regulation
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Oxidative regulation of chloroplast ATP synthase by thioredoxin-like proteins2022

    • Author(s)
      Sekiguchi Takatoshi, Keisuke Yoshida, Ken-Ichi Wakabayashi, Toru Hisabori
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Regulation
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シロイヌナズナにおける葉緑体内局在型Trx様タンパク質の非光化学的消光制御への関与2022

    • Author(s)
      福士結香,横地佑一,若林憲一,吉田啓亮,久堀徹
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Biochemical Basis for Redox Regulation of Chloroplast-Localized Phosphofructokinase from Arabidopsis thaliana2022

    • Author(s)
      吉田啓亮、久堀徹
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 光合成のレドックス制御に関わるPIFIタンパク質の分子機能解析2022

    • Author(s)
      村井美波,吉田啓亮,車玉芬,石川規子,久堀徹,伊福健太郎
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] フェレドキシン/チオレドキシン系の完全破壊株シロイヌナズナの解析2022

    • Author(s)
      吉田啓亮,横地佑一,田中寛,久堀徹
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] 色素体セリン合成系酵素3- ホスホグリセリン酸デヒドロゲナーゼのリン酸化2022

    • Author(s)
      上村柚紀子,吉田啓亮,Chris White-Gloria,Greg B. Moorhead,若林憲一,久堀徹
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] Trx およびTrx 様タンパク質による葉緑体タンパク質の酸化制御2022

    • Author(s)
      福士結香,横地佑一,若林憲一,吉田啓亮,久堀徹
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] Trx 様タンパク質による葉緑体ATP 合成酵素の不活性化制御2022

    • Author(s)
      関口敬俊,吉田啓亮,若林憲一,久堀徹
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] Important roles of PGDH-mediated serine synthesis in thallus growth, male gametogenesis and metabolism in Marchantia polymorpha2022

    • Author(s)
      Mengyao Wang, Hiromitsu Tabeta, Kinuka Ohtaka, Ayuko Kuwahara, Kiminori Toyooka, Mayuko Sato, Mayumi Wakazaki, Hiromichi Akashi, Takayuki Kohchi, Ryuichi Nishihama, Keisuke Yoshida, Ali Ferjani, Masami Yokota Hirai
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] Functional interaction between Cystathionine-β-synthase X proteins and NADPH-thioredoxin reductase C in Arabidopsis thaliana2022

    • Author(s)
      Minh Chau Tran, Shouko Mihara, Ken-ichi Wakabayashi, Keisuke Yoshida, Toru Hisabori
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] チオレドキシン様タンパク質による葉緑体ATP合成酵素の酸化制御2021

    • Author(s)
      関口敬俊, 吉田啓亮, 若林憲一, 久堀徹
    • Organizer
      第11回日本光合成学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] 光合成のチオレドキシン制御系に関するタンパク質生化学研究2021

    • Author(s)
      吉田啓亮
    • Organizer
      第一回光合成タンパク質勉強会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 光合成の恒常性を維持する葉緑体のレドックス制御機構2021

    • Author(s)
      久堀徹、吉田啓亮、横地佑一、福士結香、傅奈恵、杉浦一徳
    • Organizer
      第94回日本生化学大会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Thioredoxin-based redox regulatory network in chloroplasts2021

    • Author(s)
      Yoshida K.
    • Organizer
      IRN France-Japan Frontiers in Plant Biology: Webinar
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Trx様タンパク質による葉緑体ATP合成酵素の不活性化制御2021

    • Author(s)
      関口敬俊, 吉田啓亮, 若林憲一, 久堀徹
    • Organizer
      日本生体エネルギー研究会第47回討論会
    • Related Report
      2021 Research-status Report
  • [Presentation] チオレドキシン系を介した葉緑体の機能統御ネットワーク2021

    • Author(s)
      吉田 啓亮,横地 佑一,久堀 徹
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] レドックスを基盤とした光合成機能のオン・オフ制御2021

    • Author(s)
      吉田 啓亮
    • Organizer
      第36回植物科学シンポジウム及び第12回植物ストレス科学研究シンポジウム
    • Related Report
      2020 Research-status Report
    • Invited
  • [Book] 光合成2021

    • Author(s)
      高橋裕一郎・園池公毅・古本強 編
    • Total Pages
      224
    • Publisher
      朝倉書店
    • ISBN
      9784254171761
    • Related Report
      2021 Research-status Report
  • [Remarks] 東工大ニュース:光が当たると光合成酵素が活性化する分子メカニズム

    • URL

      https://www.titech.ac.jp/news/2022/065239

    • Related Report
      2022 Annual Research Report
  • [Remarks] 東工大化生研 田中・吉田研究室

    • URL

      http://www.res.titech.ac.jp/~biores/

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
  • [Remarks] Hisabori-Wakabayashi Laboratory

    • URL

      http://www.res.titech.ac.jp/~junkan/Hisabori_HomePage/index.html

    • Related Report
      2020 Research-status Report

URL: 

Published: 2020-08-03   Modified: 2024-01-30  

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